Thermographic and histological analysis of rabbit different tenorrhaphies techniques (4 and 6 strands) after early active mobilization

Abstract

Introduction This research is based on the results of the surgeries of tenorraphy, which have been improved due to the association between strong and not voluminous sutures and physiotherapic protocols, which preconize the early active motion to the postoperative period. Objective To evaluate the healing process in vivo in different types of tenorraphies. Methods Thirty-six rabbits that underwent early active motion after tenorraphy. The sample was constituted of 3 groups of 12, in accordance with the 3 different types of suture (Brasil, Indiana and Tsai). Results On the 15th and 30th days after the surgery, thermographic and histological analyses revealed similar results that all groups showed similar behaviors in the same time of surgical repair, just differentiating between the periods. On the 30th day analysis were observed that collagen fibers being more exuberant thickening, thus being able to offer higher tensile strength to the tendon. Conclusion That suggests early active motion may be increased gradually to around the 30th day taking this as clinical relevance.

Achilles Tendon Repair by Decellularized and Engineered Xenografts in a Rabbit Model

“…Indeed, the final concentration of the extracted proteins was certainly sufficient to detect the activity of MMP-2, but not enough to appreciate the levels of MMP-9, as also suggested by Monteiro and colleagues, who loaded only 8 μ g of protein to detect MMP-2 and 50 μ g to detect MMP-9 with scarce results [48]. Regarding biomechanics, all treated groups had inferior biomechanical properties compared to NT due to the presence of sutures used for the widely employed Kessler's tenorrhaphy [33] that could affect the elasticity of the entire implant, as expected. Moreover, the great presence of collagen type III in AG and TEG, commonly deposited during the tendon healing process, had surely affected the tendon biomechanics [49].…”

“…No significant differences were found between AG and TEG groups that showed a clearly superior tissue healing compared to the DG group. Specifically, the histological results indicate an increased concentration of collagen fibres in both AG and TEG groups, considered as an index of the healing process [33]. Similarly, the presence of a mild inflammatory pattern in these groups represents the basis for the healing process [34].…”

Termografia infravermelho da superfície abdominal ventral de coelhos (oryctolagus cuniculus) pré e pós-laparorrafia mediana com fios de quitosana ou poliglecaprone / Infrared thermography of ventral abdominal surface of rabbits (oryctolagus cuniculus) pre and post midline laparorrhaphy using chitosan or polyglecaprone yarns

“…It has been used in several anatomical regions and in different species presenting good correlation with the traditional histological evaluation method. In this scenario, infrared equipment has been Brazilian Journal of Development, Curitiba, v.7, n.4, p. 35275-35292 apr 2021 used in equines 19,20 , dogs 25 , rats 22,26 , and rabbits 27 encompassing anatomical regions of knee joint 25 , heel region 27 , carpus 21 , metacarpus 19,20 , lower lip 22 , and lateral thoracic wall 19,22 . However, most studies that evaluated inflammatory processes using infrared thermography technique did not observe a preoperative evaluation to standardize cutaneous temperature 20,[27][28][29][30] .…”

“…In this scenario, infrared equipment has been Brazilian Journal of Development, Curitiba, v.7, n.4, p. 35275-35292 apr 2021 used in equines 19,20 , dogs 25 , rats 22,26 , and rabbits 27 encompassing anatomical regions of knee joint 25 , heel region 27 , carpus 21 , metacarpus 19,20 , lower lip 22 , and lateral thoracic wall 19,22 . However, most studies that evaluated inflammatory processes using infrared thermography technique did not observe a preoperative evaluation to standardize cutaneous temperature 20,[27][28][29][30] . Moreover, in referred literature 11,12,[19][20][21][22][23][24][25][26][27] , there are no reports of evaluating ventral abdominal surface temperature, pre or post-implantation of biomaterials, furthermore, few studies used healthy animals in regulated environment with controlled temperature, luminosity, humidity, and air currents 31 .…”

“…However, most studies that evaluated inflammatory processes using infrared thermography technique did not observe a preoperative evaluation to standardize cutaneous temperature 20,[27][28][29][30] . Moreover, in referred literature 11,12,[19][20][21][22][23][24][25][26][27] , there are no reports of evaluating ventral abdominal surface temperature, pre or post-implantation of biomaterials, furthermore, few studies used healthy animals in regulated environment with controlled temperature, luminosity, humidity, and air currents 31 .…”