[Retracted Article]: Evaluation of Enemas Containing Sucralfate in Tissue Content of Muc-2 Protein in Experimental Model of Diversion Colitis

Abstract

Background: The effects of topical application of sucralfate (SCF) on the tissue content of MUC-2 protein have not yet been evaluated in experimental models of diversion colitis. Aim: To measure the tissue content of MUC-2 protein in the colonic mucosa diverted from fecal stream submitted to the SCF intervention. Methods: Thirty-six rats underwent derivation of intestinal transit through proximal colostomy and distal mucous fistula. The animals were divided into three groups which were submitted application of enemas with saline, SCF 1 g/kg/day and SCF 2 g/kg/day. Each group was divided into two subgroups, according to euthanasia was done after two or four weeks. The colitis diagnosis was established by histopathological study and the inflammatory intensity was evaluated by previously validated scale. The MUC-2 protein was identified by immunohistochemistry and the tissue content was measured computerized morphometry). Results: The application of enemas with SCF in the concentration of 2 g/kg/day reduced inflammatory score of the segments that were diverted from fecal stream. The content of MUC-2 in diverted colon of the animals submitted to the intervention with SCF, independently of intervention period and the used concentration, was significantly greater than animals submitted to the application of enemas containing saline (p< 0.01). The content of MUC-2 after the intervention with SCF in the concentration of 2 g/kg/day was significantly higher when compared to the animals submitted to the application containing SCF at concentration of 1.0 g/kg/day (p<0.01). The tissue content of MUC-2 reached the highest values after intervention with SCF in the concentration of 2 g/kg/day for four weeks (p<0.01). Conclusion: The preventive application of enemas containing SCF reduces the inflammatory score and avoids the reduction of tissue content of MUC-2, suggesting that the substance is a valid therapeutic strategy to preserve the mucus layer that covers the intestinal epithelium.

Efficacy of Curcuma Longa in the Treatment of Diversion Colitis in Rats

“…In the present study, it was observed that the exclusion of faecal transit in the colonic segment in rats for 21 days actually promoted an inflammatory process, considering the average of 2.7 degrees observed, on a scale ranging from 0 to 3, characterized by mild to severe inflammation. The same has been observed by other authors, and this is the starting point for the evaluation of the therapeutic efficacy of Curcuma longa 1 , 2 , 3 , 4 , 5 . The method of histological evaluation was also the same, which facilitates comparison, as it is well characterized by infiltration with mononuclear cells, which include macrophages, lymphocytes and plasma cells; tissue destruction by inflammatory cells and attempts at healing by the replacement of tissue damaged by connective tissue by small vessel proliferation (angiogenesis) and fibrosis 7 , 8 , 13 , 14 , 15 , 16 .…”

“…Fernandez et at 4 . evaluated the sucralfate in the treatment of exclusion colitis in rats and also started the therapy immediately after the stoma creation, which supports the method of Kadri et al 8 , but which in our view would not be ideal, because the actual efficacy of the drug is not known in the face of a more intense and already installed inflammatory process.…”

“…These diversions are mainly made on a temporary basis; however, may become definitive because of the clinical conditions of the patients. When temporary, an average of three months is estimated for intestinal reconstruction; this period may be extended for years due to multiple factors 4 , 17 .…”

Factors Related to the Reduction of the Risk of Complications in Colorectal Surgery Within Perioperative Care Recommended by the Acerto Protocol

“…Both in the ACERTO project and ERAS protocol there is a large range of routines in different moments for prescriptions and therefore it is particular interest in investigating which component of one multimodal protocol such as the ACERTO is most associated with the observed beneficial effects. This answer could help for the definitions of better clinical strategies of implementation of programs of enhancing recovery and also, be the basics for decision policies to be adopt aiming at optimization of postoperative recovery 12 , 23 .…”

Effect of Chronic Renal Dysfunction on the Permeability of the Colon to Water and Electrolytes: Experimental Study in Rats

“…Any kind of electrolyte imbalance is dangerous since the optimal functioning of the body depends on it. Low sodium (hyponatremia), for example, can cause confusion and muscle weakness 4 , 9 , 15 .…”