Taxonomic value of histochemical features of the style in early lineages of Asteraceae

Abstract

In this paper we examine lipophilic substances, tannins, proteins, reducing carbohydrates, starch, and oxalate salts in early lineages of Asteraceae to test if they are a useful complement to classification studies. Styles of mature flowers of 44 species were processed according to plant histochemical techniques and observed by means of a light microscope. Lipophilic compounds were always present in the cuticle and cell walls, and in some species were located inside the cells. Proteins were located in the style cell walls and cell interior of most of the analyzed species. No tannins were found. In general, there is a correlation in the presence of reducing sugars and proteins. Starch granules and oxalate salts were found in the style parenchyma of a few species. Cytoplasmic content of lipophilic compounds, proteins, and reducing sugars predominates in Barnadesioideae and Nassauvieae (Mutisioideae), and histochemical similarities were found among members of Gochnatioideae.

On the Presence of Uncommon Stylar Glandular Trichomes in Asteraceae: A Study in Kaunia R.M. King and H. Rob. (Oxylobinae, Eupatorieae)

“…Herbarium specimens of two selected species of Kaunia (K. longipetiolata and K. camataquiensis), in which the presence of stylar glands was confirmed by LM observations, were used for the histochemical test. Histochemical analyses on herbarium specimens have yielded successful results in other Asteraceae (Hernández et al 2015). Several flowers of each species were dissected, separating the styles.…”

Estudios en la subtribu <i>Oxylobinae</i> R. M. King & H. Rob (<i>Asteraceae</i>, <i>Eupatorieae</i>): revisión sistemática, análisis cladístico y biogeográfico del género <i>Kaunia</i> R. M. King & H. Rob

“…Especímenes de herbario de dos especies de Kaunia en las cuales la presencia de tricomas glandulares en el estilo fue confirmada por observaciones bajo el microscopio óptico fueron utilizadas para el test histoquímico. Los análisis histoquímicos realizados en materiales de herbario han probado ser exitosos en otras especies de Asteraceae (Hernández et al 2015). Varias flores de cada especie fueron diseccionadas para obtener el estilo aislado.…”