How to construct and use a simple device to prevent the formation of precipitates when using Sudan Black B for histology

Abstract

The present work aims to demonstrate the stages of fabrication and use of a simple device to avoid the formation or fixation of precipitates from Sudan Black B solution on tissues. The device consists of four coverslip fragments attached to a histology slide, which serve as points of support for the histological slide under analysis. To work properly, the histology slide with the sections should be placed with the sections facing downwards the device. A small space between the device and the histology slide is thereby created by the height of the coverslip fragments. When Sudan Black B is applied, the solution is maintained within the edges of the device and evaporation is minimized by the small space, thereby reducing the consequent formation of precipitates. Furthermore, by placing the sections facing downward the device, any sporadically formed precipitates are prevented from settling on and fixing to the sectioned tissues or organs. By avoiding the formation of precipitates, plant cells, tissues and organs can be better observed, diagnosed and photomicrographically recorded.

Morphology, anatomy, and histochemistry of three species of Jatropha: a contribution to plant recognition and selection

“…Essential oils may include >100 different combinations of terpenes and hydrocarbon terpenes, while terpenes only relate to individual monoterpenes, sesquiterpenes, or diterpenes (M ath e 2015). Sudan III, which has azo (-N=N-) and hydroxyl (OH) groups, is used to identify steroids, oils, greases, and fats (nonpolar substances) in samples due to its high affinity with nonpolar substances, producing an orange colour reaction (Sass 1951;Oliveira 2015). Inadequate differentiation among reagents used to reveal secretory structures as primary locations of production and accumulation of secondary metabolites (Bong et al 2021), or a low content of such compounds, might result in important chemicals going unrecognized.…”

Tightly Controlled Expression of bHLH142 Is Essential for Timely Tapetal Programmed Cell Death and Pollen Development in Rice

“…Sudan Black B (0.3%, w/v; Sigma, Lot#MKBQ9075V) prepared in 70% ethyl alcohol was used to stain lipids, as described previously (Oliveira, 2015). …”

“…Transverse paraffin sections of anther were sectioned, deparaffined, rehydrated, and stained for starch with 2% I 2 /KI solution. Sudan Black B (0.3%, w/v; Sigma, Lot#MKBQ9075V) prepared in 70% ethyl alcohol was used to stain lipids, as described previously ( Oliveira, 2015 ).…”