Colonização do xilema de eucalipto por Ceratocystis spp. isolado de diferentes hospedeiros

Firmino, Ana Carolina; Tanaka, Francisco André Ossamu; Silva, Stela Dalva Vieira Midlej; Ito, Margarida Fumiko; Furtado, Edson Luiz

doi:10.1590/0100-5405/2061

Cited by 2 publications

(3 citation statements)

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“…Many conidia of C. fimbriata were observed in the stem tissues of the susceptible clone at the beginning of the fungal infection process. The production of conidia during the early stages of colonization might be the key to the rapid dispersion of the fungus in plant tissues (Duncan and Himelick 1988;Firmino et al 2015). In the resistant clone, many aleuroconidia were observed, which indicated their role as resistance structures that allow the fungus to survive under adverse conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Up to now, it is known that C. fimbriata induces longitudinal and radial darkening of wood and infects the xylem and phloem vessels and phelloderm causing tree wilt (Ferreira et al 2006). Conidia, aleuroconidia, and ascospores of Ceratocystis isolates from different hosts (e.g., eucalypts, cocoa, mango, teak, and atemoya) are capable of germinating, penetrating, and developing in the vessel elements of eucalypt plants within 6 h (Firmino et al 2015). The resistance of eucalypt plants to C. fimbriata is related to enzymatic activity (e.g., polyphenol oxidase and peroxidase activities) and accumulation of sugar, antimicrobial compounds (e.g., flavone, hesperetin, loganin, and nicotinamide), and phenolic compounds (Silva et al 2018;Pimenta et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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Responses of resistant and susceptible hybrid clones of Eucalyptus urophylla × Eucalyptus grandis to infection by Ceratocystis fimbriata

Silva

Betancourth

Ferreira

et al. 2020

Annals of Forest Science

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& Key message A histopathological study using one resistant and one susceptible clone of Eucalyptus urophylla × Eucalyptus grandis hybrid showed that the colonization of Ceratocystis fimbriata was limited by rapid and intense host defense responses such as closure of the vessel pits; formation of tyloses and gels; accumulation of amorphous material, starch, phenolic compounds, and calcium oxalate; and tissue lignification. The defense mechanisms of the resistant clone were not lethal to the pathogen because the fungus was reisolated from the diseased tissue. & Context The use of resistant genotypes of eucalypt is widely used to control Ceratocystis wilt caused by Ceratocystis fimbriata. However, little is known regarding the fungal infection process and the host defense responses. & Aims Thus, the objectives of this study were to compare the histopathological responses of one resistant and one susceptible clone of Eucalyptus urophylla × Eucalyptus grandis to artificial inoculation with C. fimbriata and to identify possible host defense responses against fungal infection. & Methods Fungal colonization was analyzed by light and scanning electron microscopy. The host defense responses to artificial fungal inoculation were evaluated through histochemical analysis and determining of the lignin concentration and lesion lengths, whereas the pathogen viability was confirmed by reisolations. & Results Both tested clones showed similar responses against fungal infection but presented defense responses with different speeds and intensities. Fungal colonization was not restricted in the tissue of plants from the susceptible clone, whereas in the resistant clone, fungal colonization was limited to the xylem vessels and parenchyma around the vessels due to closure of the vessel pits; intense formation of tyloses and gels; accumulation of amorphous material, starch, phenolic compounds, and calcium oxalate; and tissue lignification. However, the fungus was reisolated from the inoculated tissues of both clones.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Responses of resistant and susceptible hybrid clones of Eucalyptus urophylla × Eucalyptus grandis to infection by Ceratocystis fimbriata

Silva

Betancourth

Ferreira

et al. 2020

Annals of Forest Science

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“…Based on these studies, it was suggested that the evolution and divergence of species of Ceratocystis may have been conducted by host specialization, since there is little morphological differences between species of these fungus (Ferreira, 2009). Analysis on electronic scanning microscope, that were aimed to monitor the colonization of five Ceratocystis isolates from different hosts (eucalyptus, cocoa, mango, teak and atemoya) on the surface of eucalyptus plants to show that all isolates were capable of germinating, penetrating and developing in the vessel elements of eucalyptus plants within 6 hours, demonstrated that these fungal isolates, even from other hosts, are capable of developing in the xylem of eucalyptus plants (Firmino et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Ceratocystis fimbriata from passion fruits

et al. 2016

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RESUMO:Frutos de maracujá (Passiflora edulis) foram encontrados com sintomas de podridão no campo, na cidade de Tanhaçu, Bahia. Após isolamento do patógeno associado a essas podridões, o presente trabalho teve como objetivo realizar a caracterização de um isolado de Ceratocystis de maracujá para melhor compreender esse patossistema. A caracterização molecular foi realizada com base no sequenciamento da região ITS-5.8S rDNA. Realizou-se a caracterização patogênica em mudas e frutos de maracujá. A colonização dos frutos de maracujá foi acompanhada com técnicas de microscopia de varredura (MEV). A análise do DNA do isolado de Ceratocystis mostrou que este pertence à espécie Ceratocystis fimbriata. As mudas de maracujá inoculadas apresentaram lesão 30 dias após a inoculação, e não foram observadas murcha nem morte das plantas inoculadas. Nos frutos não foram constatadas diferenças nas lesões causadas por esse fungo entre os cultivares, com médias de lesões de 1,0 a 2,2 cm de diâmetro, aos 7 e 11 dias, respectivamente. Nas análises realizadas em MEV, foram observadas a germinação dos esporos e a penetração do fungo nos frutos no período entre 2 e 6 horas após a inoculação. Doze e 24 horas após a inoculação foi visualizada a colonização do fruto, tanto externa como internamente, e 48 horas após a inoculação se notou o início da degradação da parede externa da casca do fruto. Noventa horas após a inoculação, observou-se a formação de novos peritécios, tanto na parte interna como na parte externa do fruto. Este estudo vem complementar as informações relacionadas à interação desse fungo com frutos de maracujazeiro. PALAVRAS-CHAVE: podridão; espécies; microscopia.ABSTRACT: Passion fruits (Passiflora edulis) were found with symptoms of rot in the field, in the city of Tanhaçu, Bahia. After isolating the pathogen associated with this rot, in the present study we aimed to characterize the Ceratocystis isolate from passion fruit for better understanding this pathosystem. Molecular characterization was done based on the region ITS-5.8S rDNA. Pathogenic characterization was carried out for seedlings and fruits of passionflower. Passion fruit colonization was monitored by means of scanning electron microscopy techniques (SEM). DNA analysis of the Ceratocystis isolate from passionflower pointed out that this species belongs to Ceratocystis fimbriata. The inoculated passionflower seedlings showed injury at 30 days post-inoculation, but no inoculated plant showed wilt or died. Considering fruits, no differences were found for lesions caused by this fungus among cultivars, and lesions had average diameters of 1.0 and 2.2 cm at 7 and 11 days, respectively. The analysis using SEM indicated fungus spore germination and penetration in the fruit between 2 and 6 hours post-inoculation. At 12 and 24 hours post-inoculation, fruit colonization was noted both externally and internally, while fruit wall degradation started at 48 hours post-inoculation. At 90 hours post-inoculation, formation of new perithecia was observed inside and outside th...

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Colonização do xilema de eucalipto por Ceratocystis spp. isolado de diferentes hospedeiros

Cited by 2 publications

References 14 publications

Responses of resistant and susceptible hybrid clones of Eucalyptus urophylla × Eucalyptus grandis to infection by Ceratocystis fimbriata

Responses of resistant and susceptible hybrid clones of Eucalyptus urophylla × Eucalyptus grandis to infection by Ceratocystis fimbriata

Characterization of Ceratocystis fimbriata from passion fruits

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