Crescimento micelial radial e esporulação de isolados de Corynespora cassiicola

Mello, Flávia Elis de; Silva, Helen Prudente da; Celestino, Gabriela Gomes; Lopes, I. de O. N.; Balbi-Peña, María Isabel; Godoy, C. V.

doi:10.1590/0100-5405/177612

Cited by 4 publications

(3 citation statements)

References 5 publications

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“…Citronella essential oil treatment allowed higher sporulation of F. oxysporum when compared to the Control (-) (Figure 3). This overproduction of spores may be associated with the stress suffered by the pathogen, as described by Mello et al (2018) when submitting Corynespora cassiicola to different light and stress regimes.…”

Section: Resultsmentioning

confidence: 97%

Volatile organic compounds (VOCs) of essential oils for the control of Fusarium oxysporum in cherry tomato seeds

Schuster-Russiano,

Russiano,

Moraes

et al. 2023

Braz. J. Biol.

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Fusarium oxysporum is the causal agent of Fusarium wilt in tomato plants. The most common form of control of this disease is through seed chemical treatment. However, the present work presents an alternative method, through the fumigation technique with essential oils. The pathogen F. oxysporum was inoculated on organic cherry tomato seeds through contact with sporulated Petri® plates. Thereafter, seeds were placed in stainless steel crucibles containing a 1.0 x 1.0 cm filter paper adhered to the lid and kept for 24 hours. This paper received 20 µL of each essential oil: tea tree, chia, citronella, lavender, anise basil, clove basil, and deionized water as control. This process was called “seed fumigation by essential oil”. After this process, a germination test was carried out in germ boxes with Germitest® paper to verify the variables Germination Speed Index (GSI), Germination (G%), and Mean time to germination (MGT). Mycelial growth was verified in Petri® plates containing PDA medium. The plates containing mycelial growth were observed through scanning electron microscopy to verify possible morphological damage in the hyphae of the pathogen. Tea tree essential oil was the one that allowed the greatest suppression of the phytopathogen. Therefore, new tests were carried out with this specific oil. In germ boxes, tests of germination (G%), Abnormal seedlings count (ASC), and percentage of seedlings with mycelial growth were carried out. In addition, plant elicitation tests were performed in tomato seedlings through the analysis of chitinase, glucanase, and total proteins. All tests were carried out in completely randomized designs with four replications. All data were submitted to the Lilliefors normality test, followed by the analysis of variance, and Tukey’s HSD (5% significance) for mean comparison. It was found that tea tree essential oil inhibited the mycelial growth of F. oxysporum without affecting the germination of cherry tomato seeds. Subsequent tests with this oil also demonstrated that there is a reduction in mycelia present in the seeds and a reduction in abnormal seedlings compared to the control. There was no significant difference between the variables tested for plant elicitation.

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Section: Resultsmentioning

confidence: 97%

Volatile organic compounds (VOCs) of essential oils for the control of Fusarium oxysporum in cherry tomato seeds

Schuster-Russiano,

Russiano,

Moraes

et al. 2023

Braz. J. Biol.

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“…The modified agar dilution method used in our study has also been used in other research, mainly to evaluate the antifungal activity of plant extracts, [29][30][31][32][33] and to compare the fungi growth in different cultivation media and conditions. 34,35 Therefore, it is an efficient, reliable, quick and highly reproducible technique.…”

Section: In Vitro Antifungal Activitymentioning

confidence: 99%

Differentiation and prediction of the in vitro antifungal activity of Brazilian propolis against Fonsecaea pedrosoi using chemometrics and diffuse reflectance infrared Fourier Transform spectroscopy

De Moraes,

Koehler,

Corbellini

et al. 2023

Braz. J. Hea. Rev.

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The in vitro antifungal activity of ethanolic extracts of propolis was evaluated at five different concentrations in twelve Fonsecaea pedrosoi strains using agar dilution. Eight propolis extracts were collected from towns located in the central region of the state of Rio Grande do Sul, Brazil. Diffuse reflectance infrared Fourier Transform spectroscopy (DRIFTS) was employed to analyze the spectra of lyophilized propolis extracts. The use of chemometric tools allowed for the development of models with more relevant factors and with fewer variables. Partial least squares (PLS) regression was applied, allowing for the development of PLS-DRIFTS models to predict antifungal activity of all fungal strains with root mean square error of cross validation (RMSECV) lower to 5.00 mm2 and coefficient correlation of cross-validation (R2) upper to 0.99 with five to eight latent variables at 4000-2400 and 2200-600 cm-1. Concluding, DRIFTS models associated with chemometrics are powerful screening tools of propolis samples with fungistatic properties against Fonsecaea pedrosoi.

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“…Desta forma, o crescimento micelial é específico para cada gênero e/ou espécie de fungo, variando conforme a temperatura (COSTA et al, 2015). O crescimento e a esporulação são afetados pelo fotoperíodo, isto é, a luz é capaz de ativar enzimas chave que induzem a produção de esporos, sendo que a qualidade e quantidade de luz necessárias para induzir a produção de estruturas reprodutivas variam conforme a espécie de fungo (MELLO et al, 2018).…”

Section: Introductionunclassified

Caracterização fisiológica de Colletotrichum gloeosporioides isolados de Mangifera indica

Santos¹,

Barboza²,

Muniz³

et al. 2023

BioCiência

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A antracnose tem sido relatada como a principal doença pré e pós-colheita da manga, atacando folhas e frutos. Este trabalho objetivou realizar a caracterização fisiológica e patogênica de cinco isolados de Colletotrichum gloeosporioides da mangueira cv. Amrapali. Para a caracterização fisiológica, os isolados foram cultivados sob diferentes regimes de luz (0h, 12h e 24h) a 25°C, em que medições diárias até o 6 DAI (Dias após inoculação) foram feitas para obtenção de medias de crescimento micelial, e aos 6 DAI quantificação de esporos. Em seguida foi avaliado o potencial patogênico dos isolados através de medições de lesões na face adaxial das folhas aos 2, 4, 6, 8 e 10 DAI. Ambas as avaliações foram conduzidas em delineamento inteiramente casualizado. Isolados de C. gloeosporioides cultivados sob o regime de 12 horas luz e sob a temperatura de 25°C obtiveram melhor desenvolvimento fisiológico e o maior desenvolvimento patogênico no regime de 0 horas de luz.

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Crescimento micelial radial e esporulação de isolados de Corynespora cassiicola

Cited by 4 publications

References 5 publications

Volatile organic compounds (VOCs) of essential oils for the control of Fusarium oxysporum in cherry tomato seeds

Volatile organic compounds (VOCs) of essential oils for the control of Fusarium oxysporum in cherry tomato seeds

Differentiation and prediction of the in vitro antifungal activity of Brazilian propolis against Fonsecaea pedrosoi using chemometrics and diffuse reflectance infrared Fourier Transform spectroscopy

Caracterização fisiológica de Colletotrichum gloeosporioides isolados de Mangifera indica

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