Caracterização morfofisiológica e patogênica de isolados de Armillaria na região Sul do Brasil

Abstract

Colonies presented variation in mycelial morphology, irregular growth without radial symmetry, coloration varying from white, to gray and to brown, and most often with rhizomorphs. Analysis of hyphae revealed typical structures of the Armillaria genus, such as clamp connections, septa, pilosities and resinous masses on the hyphal surface. Rhizomorph morphology appeared variable depending on the environment, presenting pilosities when developed in aerial growth and smooth surface when developed in the culture medium. The mycelial fan was crusted and had verrucous hyphae. Optimum temperatures for Armillaria isolates varied from 16 to 24 ºC. Only one inoculated plant died, indicating difficulty in reproducing the disease in vivo. One basidiome was produced, demonstrating the possibility of in vitro fruiting of Armillaria sp.

Biosynthesis of D-Mannitol by Diplodia pinea

“…For the production of pure cultures, in a laminar flow hood, the fungus was removed from the mineral oil, with the help of a bacteriological loop and inoculated into Petri dishes containing PDA medium. The plates were incubated in a BOD chamber at 24 ℃, in the dark, for 10 days (Silva et al, 2018).…”

Biotechnology of biomass production in vitro of fungi isolated from Pinus

“…Research, Society and Development, v. 9, n. 10, e7809109080, 2020 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v9i10.9080 Each isolate was grown in Petri dishes with potato-dextrose-agar medium -PDA (commercial extract of PDA, 39 g; ultra-purified water, 1000 mL) to produce inoculum in BOD chambers, at 24 ºC, in the dark, for 14 days. The fungi cultivation tests were performed in a liquid medium following the methodology of Silva et al (2018), varying the type of broth, temperature and time of cultivation.…”