How to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters

Bezerra, Yane Caroline dos Anjos; Marques, João Paulo Rodrigues; Stipp, Liliane Cristina Liborio; Attílio, Lísia Borges; Freitas-Astúa, Juliana; Filho, Francisco de Assis Alves Mourão

doi:10.1590/0100-29452021005

Cited by 2 publications

(3 citation statements)

References 38 publications

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“…54 This expansion is driven by the demand for areas free from huanglongbing (HLB) occurrence. 55 It is important to note that the observed temperatures in these regions fall within the susceptible range for Alternaria brown spot of 17 -33 °C. 20,56 Therefore, both established and new tangerine plantations in these states are susceptible to the occurrence of the disease.…”

Section: >33 °C Unsuitablementioning

confidence: 83%

“…Among these expanding citrus cultivation municipalities are Aporé (GO) and Aparecida do Tabuado (MS) 54 . This expansion is driven by the demand for areas free from huanglongbing (HLB) occurrence 55 . It is important to note that the observed temperatures in these regions fall within the susceptible range for Alternaria brown spot of 17 –33 °C 20,56 .…”

Section: Resultsmentioning

confidence: 99%

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Climate zoning: identifying suitable regions for the occurrence of Alternaria Brown spot in tangerine trees in Brazil

Aparecido,

Torsoni,

Lima

et al. 2023

Pest Management Science

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BACKGROUNDBrazilian citrus farming has been migrating to nontraditional citrus‐growing regions, which can be considered a challenge for citrus growers, as not all these areas are suitable for tangerine cultivation. Thus, the mapping of regions exhibiting favorable climatic conditions for Alternaria brown spot has become crucial in the selection of appropriate locations for the establishment of new orchards. This mapping enables the implementation of an avoidance strategy, which entails steering clear of areas where the disease is prevalent, aligning with fundamental principles of disease control.RESULTSThus, this study seeks to zone areas with high and low climatic favorability for the occurrence of Alternaria brown spot in tangerine trees in Brazil. Historical climate data series from the NASA‐POWER database were used for all municipalities in Brazil. Agrometeorological variables used to determine the development of Alternaria brown spot were average monthly air temperature (Tmean) and duration of leaf wetness period (LWD). Areas were considered unsuitable climatically when Tmean was <17 °C or >33 °C, relatively suitable when Tmean was between 13 °C and 33 °C and LWD <10%, and climatically suitable when Tmean was between 13 °C and 33 °C and LWD >10%. The states of Paraná, Santa Catarina and Rio Grande do Sul showed greater thermal amplitude within months and throughout the year. The southern region of the country has harsher winters, with minimum temperatures below 15 °C, which is unfavorable for the disease incidence.CONCLUSIONThe states with the greatest favorability for Alternaria brown spot were Paraná, Santa Catarina, and Rio Grande do Sul, mainly from May to September. Rio Grande do Sul was the state in this region that showed the greatest favorability, as a consequence of leaf wetness exceeding 10 h. The main tangerine‐producing regions in Brazil, including the southern part of Minas Gerais, the state of São Paulo and the metropolitan region of Porto Alegre, were mostly classified as relatively favorable areas for the occurrence of Alternaria brown spot. It is recommended that when establishing new tangerine orchards, regions with lower favorability for the occurrence of Alternaria brown spot, such as the North and Central‐West regions of Brazil, particularly the states of Amazonas, Pará and Mato Grosso, should be selected. © 2023 Society of Chemical Industry.

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Section: >33 °C Unsuitablementioning

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Climate zoning: identifying suitable regions for the occurrence of Alternaria Brown spot in tangerine trees in Brazil

Aparecido,

Torsoni,

Lima

et al. 2023

Pest Management Science

View full text Add to dashboard Cite

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“…A third requirement is that the native target gene must be linked to a suitable regulatory sequence. For that purpose, several citrus-derived promoters are being characterized ( Erpen et al, 2018 ; Dasgupta et al, 2020 ; Bezerra et al, 2021 ).…”

Section: Emerging Technologies: Cis and Intragenesis Trans -Grafting And Gene Editing In Citrusmentioning

confidence: 99%

Citrus Genetic Transformation: An Overview of the Current Strategies and Insights on the New Emerging Technologies

et al. 2021

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Citrus are among the most prevailing fruit crops produced worldwide. The implementation of effective and reliable breeding programs is essential for coping with the increasing demands of satisfactory yield and quality of the fruit as well as to deal with the negative impact of fast-spreading diseases. Conventional methods are time-consuming and of difficult application because of inherent factors of citrus biology, such as their prolonged juvenile period and a complex reproductive stage, sometimes presenting infertility, self-incompatibility, parthenocarpy, or polyembryony. Moreover, certain desirable traits are absent from cultivated or wild citrus genotypes. All these features are challenging for the incorporation of the desirable traits. In this regard, genetic engineering technologies offer a series of alternative approaches that allow overcoming the difficulties of conventional breeding programs. This review gives a detailed overview of the currently used strategies for the development of genetically modified citrus. We describe different aspects regarding genotype varieties used, including elite cultivars or extensively used scions and rootstocks. Furthermore, we discuss technical aspects of citrus genetic transformation procedures via Agrobacterium, regular physical methods, and magnetofection. Finally, we describe the selection of explants considering young and mature tissues, protoplast isolation, etc. We also address current protocols and novel approaches for improving the in vitro regeneration process, which is an important bottleneck for citrus genetic transformation. This review also explores alternative emerging transformation strategies applied to citrus species such as transient and tissue localized transformation. New breeding technologies, including cisgenesis, intragenesis, and genome editing by clustered regularly interspaced short palindromic repeats (CRISPR), are also discussed. Other relevant aspects comprising new promoters and reporter genes, marker-free systems, and strategies for induction of early flowering, are also addressed. We provided a future perspective on the use of current and new technologies in citrus and its potential impact on regulatory processes.

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How to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters

Cited by 2 publications

References 38 publications

Climate zoning: identifying suitable regions for the occurrence of Alternaria Brown spot in tangerine trees in Brazil

Climate zoning: identifying suitable regions for the occurrence of Alternaria Brown spot in tangerine trees in Brazil

Citrus Genetic Transformation: An Overview of the Current Strategies and Insights on the New Emerging Technologies

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