Occurrence of Purpureocillium lilacinum in citrus black fly nymphs

Abstract

Black fly is a pest of Asian origin that causes direct and indirect damages to citrus, damaging the development and production of plants. For the development of efficient management strategies of the pest, the integration of control methods is necessary, and biological control is the most appropriate. Among the agents that can be used, entomopathogenic fungi are considered one of the most important and wide-ranging use. This work investigated the occurrence of Purpureocillium lilacinum (Thom.) Luangsa-ard et al. (= Paecilomyces lilacinus), attackingnymphs of citrus black fly, Aleurocanthus woglumi Ashby (Hemiptera: Aleyrodidae). The fungus was isolated from infected Black fly nymphs, present on Citrus spp leaves in the municipality of Morros, Maranhão. After isolation, purification and morphological and molecular characterization, pathogenicity test was performed with A. woglumi nymphs. Morphological and molecular correspondence was verified between inoculum and the reisolated, proving the pathogenicity of P. lilacinum.

Biodiversity of Entomopathogenic Fungi in the Soils of South China

“…Purpureocillium lilacinum is a combination with obvious intraspecific diversity. Many strains of the species have been used as biocontrol agents of phytopathogenic nematodes [58,59], while some strains have insecticidal potency [60] and some others have the risks to cause infection in immunocompromised humans [61,62]. In this experiment, P. lilacinum is also the most abundant species isolated from whole six regions with the most 75 isolates, in which the differentiation of the species is found from the phylogenetic tree constructed with these isolates.…”

Entomopathogenicity of fungi in combination with Ricinus communis extract for the control of Aleurocanthus woglumi

“…Luangsa‐ard et al. has been reported in natural epizootics against A. woglumi nymphs (Medeiros et al., 2018).…”

Endophytic fungi of olive tree and their exploitation in the biological control of olive anthracnose = Hongos endofíticos del olivo y su aprovechamiento para el control biológico de la antracnosis del olivo

“…Del total de UTOs, un 44% fueron exclusivas de raíces, con solo un 9% compartido entre los tres órganos. (Medeiros et al, 2018), y en menor medida patógenos de plantas (Crinipellis, Ilyonectria y Phaeosphaeria) (Da Silva, 2004;Lombard et al, 2013…”

“…Indeed, the endophytes most positively associated to the anthracnose-susceptible cultivar are from genera that include plant pathogens, such as Chromelosporium (Mukobata andSaioto 1996), Fusarium (Moretti, 2009) and Phomopsis (Udayanga et al, 2011), as well as ubiquitous fungi widely present in various processes from necrotrophic pathogenicity to endophytic mutualism (Penicillium) (Visagie et al, 2014), and in less extent biocontrol agents (Trichoderma) (Carrero-Carrón et al, 2016). In contrast, the fungal genera most positively associated to anthracnose-moderately susceptible or -resistant cultivars, include members with recognized biocontrol abilities against plant diseases (Epicoccum, Aureobasidium, Cladosporium and Hypocrea) (Musetti et al, 2011;Elgorban et al, 2014;Khan et al, 2016;Rathnayake et al, 2018) and insect pests (Purpureocillium) (Medeiros et al, 2018), and in less extent plant pathogens (Crinipellis, Ilyonectria and Phaeosphaeria) (Da Silva, 2003;Lombard et al, 2013). Curiously, these genera used as biocontrol agents were also found to be positively associated with aboveground olive organs.…”