Influence of age on the haemoglobin concentration of malaria-infected patients in a reference centre in the Brazilian Amazon

Siqueira, André Machado; Cavalcante, Janieldo A; Vítor-Silva, Shelia; Reyes-Lecca, Roberto Carlos; Alencar, Aline; Monteiro, Wuelton Marcelo; Alexandre, Márcia Aa; G, Mourão Maria Paula; Guinovart, Caterina; Bassat, Quique; Alecrim, Maria das Graças Costa; Lacerda, Marcus

doi:10.1590/0074-0276140132

Cited by 9 publications

(8 citation statements)

References 36 publications

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“…The mean haemoglobin (Hb) of the studied subjects was significantly lower than that of the control group ( p = 0.037). This finding agrees with a study in the Brazilian Amazon in which out of a total of 7831 people studied, individuals with malaria were seen to have the lowest Hb compared to community controls and malaria-negative febrile patients [21]. The results of our study also collaborate with the results from a study carried out in Nigeria [22].…”

Section: Discussionsupporting

confidence: 92%

Proteomic analysis of microparticles isolated from malaria positive blood samples

et al. 2016

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BackgroundMalaria continues to be a great public health concern due to the significant mortality and morbidity associated with the disease especially in developing countries. Microparticles (MPs), also called plasma membrane derived extracellular vesicles (PMEVs) are subcellular structures that are generated when they bud off the plasma membrane. They can be found in healthy individuals but the numbers tend to increase in pathological conditions including malaria. Although, various studies have been carried out on the protein content of specific cellular derived MPs, there seems to be paucity of information on the protein content of circulating MPs in malaria and their association with the various signs and symptoms of the disease. The aim of this study was therefore to carry out proteomic analyses of MPs isolated from malaria positive samples and compare them with proteins of MPs from malaria parasite culture supernatant and healthy controls in order to ascertain the role of MPs in malaria infection.MethodsPlasma samples were obtained from forty-three (43) malaria diagnosed patients (cases) and ten (10) healthy individuals (controls). Malaria parasite culture supernatant was obtained from our laboratory and MPs were isolated from them and confirmed using flow cytometry. 2D LC-MS was done to obtain their protein content. Resultant data were analyzed using SPSS Ver. 21.0 statistical software, Kruskal Wallis test and Spearman’s correlation coefficient r.ResultsIn all, 1806 proteins were isolated from the samples. The MPs from malaria positive samples recorded 1729 proteins, those from culture supernatant were 333 while the control samples recorded 234 proteins. The mean number of proteins in MPs of malaria positive samples was significantly higher than that in the control samples. Significantly, higher quantities of haemoglobin subunits were seen in MPs from malaria samples and culture supernatant compared to control samples.ConclusionA great number of proteins were observed to be carried in the microparticles (MPs) from malaria samples and culture supernatant compared to controls. The greater loss of haemoglobin from erythrocytes via MPs from malaria patients could serve as the initiation and progression of anaemia in P.falciparum infection. Also while some proteins were upregulated in circulating MPs in malaria samples, others were down regulated.

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Section: Discussionsupporting

confidence: 92%

Proteomic analysis of microparticles isolated from malaria positive blood samples

et al. 2016

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“…Studies in areas of low malaria transmission in South Africa and India showed an association between severe malaria and HIV 78,[81][82][83] . For severe vivax malaria, the current study showed a higher prevalence compared to another study with severe vivax malaria in children and adult patients (23.8% versus 12.6%) 84 . Despite the low number of cases, HIV co-infection seems to exacerbate clinical worsening of vivax malaria, as it has a higher prevalence than that found in vivax malaria mono-infection patients treated at FMT-HVD (approximately 14%) 56 .…”

Section: Discussioncontrasting

confidence: 59%

Should we care about Plasmodium vivax and HIV coinfection? A systematic review and a cases series from the Brazilian Amazon

Del-Tejo¹,

Cubas-Vega²,

Caraballo-Guerra³

et al. 2020

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Background. Malaria and HIV are two important public health issues. However, evidence on HIV-Plasmodium vivax co-infection (HIV/PvCo) is scarce, with most of the available information related to P. falciparum on the African continent. It is unclear whether HIV can change the clinical course of Plasmodium vivax (Pv) malaria, and increase the risk of complications. In this study, we present a systematic review of the HIV/PvCo, and include recent cases from the Brazilian Amazon. Methods. Medical records from a tertiary care center in the Western Brazilian Amazon (2009 - 2018) were reviewed to identify HIV/PvCo hospitalized patients. Demographic, clinical and laboratory characteristics and outcomes are reported. We also performed a systematic review of published studies on HIV/PvCo. Metadata, number of HIV/PvCo cases, demographic, clinical, and outcome data were extracted. Results. A total of 1048 vivax malaria patients were hospitalized in the 10-year period; 21 (2.0%) were HIV/PvCo cases, of which nine (42.9%) had AIDS-defining illnesses. This was the first malaria episode in eleven (52.4%) patients. Seven (33.3%) patients were unaware of their HIV status and were diagnosed on hospitalization. Severe malaria was diagnosed in 5 (23.8%) patients. One patient died. The systematic review search provided 17 articles (12 cross-sectional or longitudinal studies and 5 case report studies). A higher prevalence of studies involved cases in African and Asian countries (35.3% and 29.4%, respectively), and the prevalence of reported co-infections ranged from 0.1 to 60%. Conclusion. Reports of HIV/PvCo are scarce in the literature, with only a few studies describing clinical and laboratory outcomes. Systematic screening for both co-infections is not routinely performed, and therefore, the real prevalence of HIV/PvCo is unknown. This study showed a low prevalence of HIV/PvCo, despite the high prevalence of malaria and HIV locally. Even though relatively small, this is the largest case series to describe HIV/PvCo.

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“…Studies in areas of low malaria transmission in South Africa and India showed an association between severe malaria and HIV 71,74−76 . For severe vivax malaria, the current study showed a higher prevalence compared to another study with vivax severe malaria in children and adult patients (23.8% versus 12.6%) 77 . Despite the low number of cases, HIV co-infection seems to act as a booster for the clinical worsening of vivax malaria, as it has a higher prevalence than that found in vivax malaria mono-infection patients treated at FMT-HVD (approximately 14%) 78 .…”

Section: Discussioncontrasting

confidence: 58%

Should We Care About Plasmodium Vivax and HIV Coinfection? A Systematic Review and a Cases Series From the Brazilian Amazon

Del-Tejo

Cubas-Vega

Caraballo-Guerra

et al. 2020

Preprint

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Background. Malaria and HIV are two important public health issues. However, data on HIV-Plasmodium vivax co-infection (HIV/PvCo) is scarce, with most of the available data related to P. falciparum in the African region. It is unclear whether HIV can change the clinical course of Plasmodium vivax (Pv) malaria, and thereby increase the risk of complications. In this study, a systematic review of the HIV/PvCo is presented, including new cases from the Brazilian Amazon. Methods. Medical records from a tertiary care center in the Western Brazilian Amazon (2009 to 2018) were reviewed to identify HIV/PvCo hospitalized patients. Demographic, clinical and laboratory characteristics, and outcomes are reported. We also performed a systematic review of published studies on HIV/PvCo. Metadata, number of HIV/PvCo cases, demographic, clinical, and outcome data were extracted if available. Results. A total of 1048 vivax malaria patients were hospitalized in the 10-year period; 21 (2.0%) were HIV/PvCo cases, of which nine (42.9%) had AIDS-defining illnesses. For eleven (52.4%) patients, this was their first malaria infection. Seven (33.3%) patients were unaware of their HIV status and were diagnosed at hospitalization. Severe malaria criteria were found in 5 (23.8%) patients. One patient died. The systematic review provided 17 articles (12 cross-sectional or longitudinal studies and 5 case report studies). A higher prevalence of studies involved cases in African and Asian countries (35.3% and 29.4%, respectively), and the prevalence of reported co-infections ranged from 0.1 to 60%. Cases of severe malaria-HIV coinfection were not reported. Conclusion. Reports of HIV/PvCo are scarce in the literature, with only a few studies describing clinical and laboratory outcomes. Systematic screening for both co-infections are not performed, and therefore, a realistic prevalence of HIV/PvCo is absent. This study showed a low prevalence of HIV/PvCo, despite local malaria and HIV high prevalence. Even though relatively small, this is the largest case series to describe HIV/PvCo.

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Influence of age on the haemoglobin concentration of malaria-infected patients in a reference centre in the Brazilian Amazon

Cited by 9 publications

References 36 publications

Proteomic analysis of microparticles isolated from malaria positive blood samples

Proteomic analysis of microparticles isolated from malaria positive blood samples

Should we care about Plasmodium vivax and HIV coinfection? A systematic review and a cases series from the Brazilian Amazon

Should We Care About Plasmodium Vivax and HIV Coinfection? A Systematic Review and a Cases Series From the Brazilian Amazon

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