Coagulase-negative staphylococci strains resistant to oxacillin isolated from neonatal blood cultures

Pereira, Valéria Cataneli; Cunha, Maria de Lourdes Ribeiro de Souza da

doi:10.1590/0074-0276130644

Cited by 11 publications

(11 citation statements)

References 22 publications

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“…A similar frequency was reported by Machado et al (2007) , suggesting that MR S. epidermidis (MRSE) carrying this SCC mec type is not prevalent in Brazil. SCC mec type I, a hospital-associated type, was frequent (24.1%); this finding stands in contrast to previous studies in which type I was found to be rare ( Ibrahem et al 2009 , Barbier et al 2010 , Zong et al 2011 ), but is similar to the findings reported in other Brazilian studies ( Machado et al 2007 , Pereira & Cunha 2013 , Ternes et al 2013) . These findings might be related to the local epidemiology of MRSE, indicating a higher frequency of SCC mec type I in Brazilian hospitals.…”

Section: Discussionsupporting

confidence: 82%

Reduced susceptibility to vancomycin and biofilm formation in methicillin-resistant Staphylococcus epidermidis isolated from blood cultures

Pinheiro¹,

Brito²,

Pereira³

et al. 2014

Mem. Inst. Oswaldo Cruz

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This study aimed to correlate the presence of ica genes, biofilm formation and antimicrobial resistance in 107 strains of Staphylococcus epidermidis isolated from blood cultures. The isolates were analysed to determine their methicillin resistance, staphylococcal cassette chromosome mec (SCCmec) type, ica genes and biofilm formation and the vancomycin minimum inhibitory concentration (MIC) was measured for isolates and subpopulations growing on vancomycin screen agar. The mecA gene was detected in 81.3% of the S. epidermidis isolated and 48.2% carried SCCmec type III. The complete icaADBC operon was observed in 38.3% of the isolates; of these, 58.5% produced a biofilm. Furthermore, 47.7% of the isolates grew on vancomycin screen agar, with an increase in the MIC in 75.9% of the isolates. Determination of the MIC of subpopulations revealed that 64.7% had an MIC ≥ 4 μg mL-1, including 15.7% with an MIC of 8 μg mL-1 and 2% with an MIC of 16 μg mL-1. The presence of the icaADBC operon, biofilm production and reduced susceptibility to vancomycin were associated with methicillin resistance. This study reveals a high level of methicillin resistance, biofilm formation and reduced susceptibility to vancomycin in subpopulations of S. epidermidis. These findings may explain the selection of multidrug-resistant isolates in hospital settings and the consequent failure of antimicrobial treatment.

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Section: Discussionsupporting

confidence: 82%

Reduced susceptibility to vancomycin and biofilm formation in methicillin-resistant Staphylococcus epidermidis isolated from blood cultures

Pinheiro¹,

Brito²,

Pereira³

et al. 2014

Mem. Inst. Oswaldo Cruz

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“…In our study, six isolates had two types: III+ I (n = 5, 11.4%) and IV+ III (n = 1, 2.3%). This was not surprising as the co-existence of two SCCmec elements appears to be common in MR-CoNS [25,39,40]. In our study, carriage of SCCmec elements was not associated with resistance to tested antibiotics.…”

Section: Discussionsupporting

confidence: 64%

Investigation of SCCmec types I–IV in clinical isolates of methicillin-resistant coagulase-negative staphylococci in Ahvaz, Southwest Iran

et al. 2020

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Today methicillin resistant coagulase-negative staphylococci (MR-CoNS) are important in terms of causing significant nosocomial infections. Besides, MR-CoNS are confirmed as the reservoir of SCCmec elements that carry mecA (methicillin-resistant) gene. Hence, the present study was designed to evaluate the susceptibility pattern, prevalence and diversity of SCCmec types I, II, III, and IV in MR-CoNS strains. In this cross-sectional study, 44 clinical isolates of MR-CoNS were identified using the cefoxitin disc method and further confirmation by polymerase chain reaction (PCR) amplification of the mecA gene. Antimicrobial susceptibility of isolates was investigated by disc diffusion. The identification of CoNS was done by amplification and sequencing of the tuf gene. Multiplex PCR method was done for the determination of SCCmec types. In the present study, the Staphylococcus epidermidis and Staphylococcus haemolyticus were the most predominant isolates with a prevalence of 45.4%. The highest resistance rates were observed against erythromycin (84.1%) and clindamycin (75%). Multiplex PCR revealed the SCCmec type I as the predominant type in the present study. Our study showed that there was no significant relationship between the presence of different types of SCCmec elements and resistance to antibiotics. The present study highlighted a frequent prevalence of MR-CoNS harboring SCCmec type genes in Ahvaz, southwest of Iran. Thus, the molecular typing and periodical monitoring of their drug resistance pattern should be considered in national stewardship programs to designing useful antibiotic prescription strategies.

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“…Other studies suggest that disc diffusion and agar dilution methods are reliable methods for the detection of methicillin resistance in CoNS. 43 , 44…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance and mecA Gene Characterization of Coagulase-negative Staphylococci Isolated from Clinical Samples in Nepal

Shrestha¹,

Bhattarai²,

Rai³

et al. 2020

IDR

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Background Coagulase-negative Staphylococci (CoNS) are a significant cause of hospital-acquired and foreign-body-related infections. We conducted this research to assess methicillin susceptibility of CoNS by disc diffusion, agar dilution, and polymerase chain reaction (PCR) methods and to assess the antimicrobial susceptibility pattern. Methods We received 123 CoNS isolates from different specimens including blood, endotracheal tube, and central venous catheter. We performed sample processing, identification, and characterization following standard guidelines. Antimicrobial susceptibility was tested based on clinical and laboratory standards institute guidelines. We detected methicillin-resistant coagulase-negative staphylococci (MRCoNS) through mecA gene, disc diffusion method, and agar dilution method and compared the accuracy with PCR as reference. Results We detected eight species of CoNS with Staphylococcus epidermidis as the most common. Most of the samples were received from the intensive care unit and blood was the dominant specimen followed by endotracheal-tube aspirate. Seventy-one percentage of isolates were methicillin-resistant by PCR method; disc diffusion and agar dilution method detected methicillin resistance with an accuracy of 96.7% and 98.3%, respectively. Antimicrobial susceptibility revealed an association between the different origins of samples, and also among the types of sample. Similarly, a comparison of the degree of resistance of antimicrobial agents between mecA gene positive and negative isolates showed significant differences. Vancomycin, linezolid, and teicoplanin are still effective for treating MRCoNS. Conclusion CoNS are a crucial cause of human infections especially in an intensive care unit setup where the use of devices is common. Disc diffusion and agar dilution are reliable for the detection of MRCoNS. The degree of antimicrobial resistance is much higher in organisms obtained from intensive care unit and foreign-body-related infections.

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Coagulase-negative staphylococci strains resistant to oxacillin isolated from neonatal blood cultures

Cited by 11 publications

References 22 publications

Reduced susceptibility to vancomycin and biofilm formation in methicillin-resistant Staphylococcus epidermidis isolated from blood cultures

Reduced susceptibility to vancomycin and biofilm formation in methicillin-resistant Staphylococcus epidermidis isolated from blood cultures

Investigation of SCCmec types I–IV in clinical isolates of methicillin-resistant coagulase-negative staphylococci in Ahvaz, Southwest Iran

<p>Antibiotic Resistance and <em>mecA</em> Gene Characterization of Coagulase-negative <em>Staphylococci</em> Isolated from Clinical Samples in Nepal</p>

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