Mycobacteria mobility shift assay: a method for the rapid identification of Mycobacterium tuberculosis and nontuberculous mycobacteria

Objective:The incidence of lung disease caused by nontuberculous mycobacteria (NTM) has been increasing worldwide. In Brazil, there are few studies about nontuberculous mycobacterial lung disease (NTMLD), and its prevalence is yet to be known. Our objective was to determine the specific etiology of the disease in the state of Rio Grande do Sul, Brazil, as well as the frequency and diversity of NTM species in our sample of patients. Methods:This is a retrospective analysis of the medical records of patients diagnosed with NTMLD treated in a referral center located in the city of Porto Alegre, Brazil, between 2003 and 2013. Results:Our sample comprised 100 patients. The most prevalent NTM species were Mycobacterium avium complex (MAC), in 35% of the cases; M. kansasii, in 17%; and M. abscessus, in 12%. A total of 85 patients had received previous treatment for tuberculosis. Associated conditions included structural abnormalities in the lungs, such as bronchiectasis, in 23% of the cases; COPD, in 17%; and immunosuppressive conditions, such as AIDS, in 24%. Conclusions:MAC and M. kansasii were the most prevalent species involved in NTMLD in the state, similarly to what occurs in other regions of Brazil. Data on regional epidemiology of NTMLD, its specific etiology, and associated conditions are essential to establish appropriate treatment, since each species requires specific regimens. Most patients with NTMLD had received previous tuberculosis treatment, which might lead to development of resistance and late diagnosis.

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“…NTMLD requires specific diagnosis to define the therapeutic regimen, given that different species of NTM require distinct therapies. 10 - 12 …”

Section: Introductionmentioning

confidence: 99%

Nontuberculous mycobacterial lung disease in a high tuberculosis incidence setting in Brazil

Carneiro

Nunes

David³

et al. 2018

J. bras. pneumol.

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“…In addition to that, several gene targets, including rpoB gene [124][125][126], secA1 gene [127] and hsp65 Mycobacterium -Research and Development gene [128], have used for NTM species identification by DNA sequencing. Also, gyrB-based microarray [129], mycobacteria mobility shift assay (MMSA) [130], biochip assay system [131] and multiplex SNaPshot assay [132] have been proven as rapid detection methods to identify closely related mycobacterial species with satisfied level of sensitivity and specificity, which may be useful in the diagnosis and effective management of NTM lung disease [129][130][131][132].…”

Section: Molecular-based Identification Methodsmentioning

confidence: 99%

Overview of Non Tuberculosis Mycobacterial Lung Diseases

Diseases¹

2018

Mycobacterium - Research and Development

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Nontuberculosis mycobacteria (NTM) are ubiquitous in nature and opportunistically infect different animals, including humans. Currently, NTM is emerging as an important cause of pulmonary infection among both immunocompromised and immunocompetent persons worldwide. The clinical relevance of pulmonary NTM varies among species while showing geographical heterogeneity in distribution as well as pathogenicity. The outcome of the respiratory NTM disease is a consequence of a complex interplay between microbial factors and host susceptibility. Furthermore, HIV infection, cystic fibrosis, cancer, underlying chronic lung disease and history of tuberculosis (TB) may be associated as risk factors for active nontuberculosis pulmonary diseases (NTMPD). The diagnosis of NTMPD requires the presence of symptoms, radiographic evidences, microscopic observations and definitive laboratory diagnostics. Lung infections resulted from a clinically significant NTM species should be treated with appropriate antimicrobial regimen. M. leprae and M. lepraemurium are uncultivable and require the intracellular milieu for survival and propagation [4]. The obligatory causative agents of the genus Mycobacterium, responsible for TB are classified into Mycobacterium tuberculosis complex (MTC). It comprises M. tuberculosis, M.bovis, M. africanum, M. microti [5], M. canettii [6], M. caprae [7], M. pinnipedii [8], M. mungi [9], M. orygis [10] and M. suricattae [11] species. M. tuberculosis, M. africanum, M. canettii and M. orygis cause TB primarily in human [4, 10], whereas M. bovis [12], M. microti [13], M. caprae [7] M. pinnipedii [8], M. mungi [9] and M. suricattae [11] infect cattle, domestic animals, goats, seals, mongooses and meerkats, respectively, and animal tuberculosis can also be zoonotic [14, 15]. However, geographical variation of the MTC species distribution has been identified. As an example, M. africanum is a common cause of human pulmonary TB (39%) as much as M. tuberculosis (55%) in West Africa [16]. In Ghana, 3% of pulmonary TB cases are represented by M. bovis, while 20% are M. africanum and 73% are M. tuberculosis [17].Other medically important mycobacteria such as M. avium, M. intracellulare complex, M. kansasii, M. marinum, M. fortuitum, M. chelonae complex, M. abscessus and M. scrofulaceum are known as nontuberculosis mycobacteria (NTM) species or atypical mycobacteria or mycobacteria other than tuberculosis (MOTT). They are responsible for diseases including lymphadenitis in children, chronic pulmonary diseases, skin and soft-tissue diseases and infections of the skeletal system [18]. NTM are ubiquitous in nature and are widely distributed in water, soil and animals. Among prevailing NTM species, only a few species have a clinical impact on humans as opportunistic pathogens [19]. M. avium complex (MAC), M. abscessus, M. kansasii, M. fortuitum, M. chelonae, M. szulgai, M. triviale and M. scrofulaceum are common NTM species that cause pulmonary diseases in human [20]. Additionally, M. riyadhense was recently proposed as a cau...

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“…Como resultado del aumento mundial del uso de técnicas como la secuenciación de RNA ribosomal 16S, más de 50 nuevas especies fueron descriptas a partir de 1990 (Schinsky et al, 2004). En la actualidad se han identificado más de 150 especies y subespecies, muchas de las cuales se relacionan con enfermedades en humanos (MuraroWildner et al, 2014). El espectro de patogenicidad varía ampliamente entre las especies, siendo algunas patógenas estrictas (M. tuberculosis, M. leprae) y otras saprófitas, así como también su reservorio, que pueden ser el hombre, los animales o el ambiente (Han et al, 2007).…”

Section: _____________________ Introducción _____________________unclassified

Lipid composition of cell wall of three species of environmental mycobacteria and its possible role in the biofilm formations and motility by sliding

Oriani¹,

Gentili²,

Zuniga³

et al. 2015

cienvet

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The wall structures of Mycobacteria are usually associated with the ability to form biofilm and motility by sliding. This study was performed Composición lipídica de la pared celular de tres especies de micobacterias ambientales y su posible correlación con la formación de biofilms y movilidad por sliding Composición lipídica de la pared celular de tres especies de micobacterias ambientales y su posible correlación con la formación de biofilms y movilidad por sliding

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Mycobacteria mobility shift assay: a method for the rapid identification of Mycobacterium tuberculosis and nontuberculous mycobacteria

Cited by 5 publications

References 25 publications

Nontuberculous mycobacterial lung disease in a high tuberculosis incidence setting in Brazil

Nontuberculous mycobacterial lung disease in a high tuberculosis incidence setting in Brazil

Overview of Non Tuberculosis Mycobacterial Lung Diseases

Lipid composition of cell wall of three species of environmental mycobacteria and its possible role in the biofilm formations and motility by sliding

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