A rapid diagnostic test for schistosomiasis mansoni

Mello-Silva, Clélia Christina; Joao, Roberto Carlos; Augusto, Ronaldo de Carvalho; Santos, Cláudia Portes

doi:10.1590/0074-0276130335

Cited by 4 publications

(4 citation statements)

References 8 publications

(9 reference statements)

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“…The sensitivity of the test can be improved by using multiple stool samples over several consecutive days, but this can compromise its simplicity and cost-effectiveness, and this modified approach can suffer from low compliance due to the reluctance of individuals to provide more than one stool sample for examination (57). While modifications to the KK test to improve the test efficacy have been suggested (65,66), the inherent drawbacks have yet to be resolved.…”

Section: Direct Parasitological Diagnosismentioning

confidence: 99%

Advances in the Diagnosis of Human Schistosomiasis

et al. 2015

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SUMMARY Schistosomiasis is a major neglected tropical disease that afflicts more than 240 million people, including many children and young adults, in the tropics and subtropics. The disease is characterized by chronic infections with significant residual morbidity and is of considerable public health importance, with substantial socioeconomic impacts on impoverished communities. Morbidity reduction and eventual elimination through integrated intervention measures are the focuses of current schistosomiasis control programs. Precise diagnosis of schistosome infections, in both mammalian and snail intermediate hosts, will play a pivotal role in achieving these goals. Nevertheless, despite extensive efforts over several decades, the search for sensitive and specific diagnostics for schistosomiasis is ongoing. Here we review the area, paying attention to earlier approaches but emphasizing recent developments in the search for new diagnostics for schistosomiasis with practical applications in the research laboratory, the clinic, and the field. Careful and rigorous validation of these assays and their cost-effectiveness will be needed, however, prior to their adoption in support of policy decisions for national public health programs aimed at the control and elimination of schistosomiasis.

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Section: Direct Parasitological Diagnosismentioning

confidence: 99%

Advances in the Diagnosis of Human Schistosomiasis

et al. 2015

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“…Urine and faecal samples were collected from 288 individuals and analyzed. Fecal samples were investigated for Schistosoma mansoni ova using the modified Kato thick smear technique 7 while urine samples were examined for Schistosoma hematobium using centrifugation followed by examination of the urinary sediments.…”

Section: Materials and Methodmentioning

confidence: 99%

Untitled

2014

IJPSR

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Schistosomiasis is a major communicable disease with a great impact on both public health and socioeconomic status of the developing world. It is highly prevalent in tropics and causes morbidity and mortality in developing countries including Sudan. Because of its chronicity and robust immunomodulatory activity, the effects of Schistosomiasis on other diseases such as allergies, autoimmunity and infectious diseases have been studied extensively in both epidemiological and experimental settings. Salmonella spp. is a common water-and food-borne cause of gastrointestinal and systemic diseases worldwide. Approximately, 2 million individuals die each year of diarrhea and Salmonella is a leading cause of this illness. A relationship between persistent as well as recurrent Salmonella bacteremia and schistosomiasis has been described both in adults and children. The present cross-sectional study was conducted to determine the relationship between schistosomiasis and salmonellosis in Helat Mahajoub in AL-Gezira Agricultural Scheme. Urine and faecal samples were collected from 288 individuals and investigated for Schistosoma ova using the modified Kato thick smear technique and the concentration technique. 207 subjects were positive for schistosomiasis and were thence subjected to bacteriological cultures of S. typhi, S. paratyphi A and S. paratyphi B. Salmonella species were detected in 64 schistosomiasis patients (30.9%). Salmonella infection was found to increase when the duration of schistosomiasis infestation was more than one year.

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“…Over 20 million people suffer from symptoms of severe schistosomiasis, including serious hormonal, physiological, and immunological complications [ 1 , 2 , 3 , 4 ]. Infection can reduce resistance to HIV and malaria and increase rates of anaemia, malnutrition, and delayed development in children [ 5 , 6 , 7 ]. The main method of managing schistosomiasis is praziquantel, a highly efficacious chemotherapeutic drug that produces few serious side effects [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of Putative Neuropeptides That Alter the Behaviour of Schistosoma mansoni Cercariae

Fogarty

Suwansa‐ard

Phan

et al. 2022

Biology

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Elucidating the infectivity of Schistosoma mansoni, one of the main etiological agents of human schistosomiasis, requires an improved understanding of the behavioural mechanisms of cercariae, the non-feeding mammalian infective stage. This study investigated the presence and effect of cercariae-derived putative neuropeptides on cercarial behaviour when applied externally. Cercariae were peptidomically analysed and 11 neuropeptide precursor proteins, all of which were specific to the Schistosoma genus and most of which highly expressed in the cercarial stage, were identified in cercariae for the first time. Protein–protein interaction analysis predicted the interaction of various neuropeptide precursors (e.g., Sm-npp-30, Sm-npp-33, Sm-npp-35) with cercarial structural proteins (e.g., myosin heavy chain and titin). In total, nine putative neuropeptides, selected based on their high hydrophobicity and small size (~1 kilodalton), were tested on cercariae (3 mg/mL) in acute exposure (1 min) and prolonged exposure (360 min) behavioural bioassays. The peptides AAYMDLPW-NH2, NRKIDQSFYSYY-NH2, FLLALPSP-OH, and NYLWDTRL-NH2 stimulated acute increases in cercarial spinning, stopping, and directional change during active states. However, only NRKIDQSFYSYY-NH2 caused the same behavioural changes at a lower concentration (0.1 mg/mL). After prolonged exposure, AAYMDLPW-NH2 and NYLWDTRL-NH2 caused increasing passive behaviour and NRKIDQSFYSYY-NH2 caused increasing body-first and head-pulling movements. These findings characterise behaviour-altering novel putative neuropeptides, which may inform future biocontrol innovations to prevent human schistosomiasis.

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A rapid diagnostic test for schistosomiasis mansoni

Cited by 4 publications

References 8 publications

Advances in the Diagnosis of Human Schistosomiasis

Advances in the Diagnosis of Human Schistosomiasis

Untitled

Identification of Putative Neuropeptides That Alter the Behaviour of Schistosoma mansoni Cercariae

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