Analysing ambiguities in trypanosomatids taxonomy by barcoding

Boucinha, Carolina; Caetano, Amanda Rodrigues; Santos, Helena Lc; Helaers, Raphaël; Vikkula, Miikka; Branquinha, Marta H.; Santos, André Luis Souza dos; Grellier, Philippe; Morelli, Karina Alessandra; d’Avila-Levy, Claudia M.

doi:10.1590/0074-02760200504

Cited by 4 publications

(3 citation statements)

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“…This is further supported by the first detection of Crithidia acanthocephali Hanson and McGhee, 1961 in bumblebees, a protozoan that was recently found to be rather frequent in Apis mellifera Linnaeus, 1758 colonies following the same Ion PGM protocol (Bartolomé et al ., 2020). A revision of taxonomic ambiguities by DNA barcoding (Boucinha et al ., 2020) proved that the sequences of this trypanosomatid were identical to those of Crithidia flexonema Wallace, Clark, Dyer and Collins 1960, so from now on both names are used for this taxon.…”

Section: Resultsmentioning

confidence: 91%

Wide diversity of parasites in Bombus terrestris (Linnaeus, 1758) revealed by a high‐throughput sequencing approach

Bartolomé

Jabal-Uriel

Buendía-Abad

et al. 2020

Environmental Microbiology

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Summary Assessing the extent of parasite diversity requires the application of appropriate molecular tools, especially given the growing evidence of multiple parasite co‐occurrence. Here, we compared the performance of a next‐generation sequencing technology (Ion PGM ™ System) in 12 Bombus terrestris specimens that were PCR‐identified as positive for trypanosomatids (Leishmaniinae) in a previous study. These bumblebees were also screened for the occurrence of Nosematidae and Neogregarinorida parasites using both classical protocols (either specific PCR amplification or amplification with broad‐range primers plus Sanger sequencing) and Ion PGM sequencing. The latter revealed higher parasite diversity within individuals, especially among Leishmaniinae (which were present as a combination of Lotmaria passim, Crithidia mellificae and Crithidia bombi), and the occurrence of taxa never reported in these hosts: Crithidia acanthocephali and a novel neogregarinorida species. Furthermore, the complementary results produced by the different sets of primers highlighted the convenience of using multiple markers to minimize the chance of some target organisms going unnoticed. Altogether, the deep sequencing methodology offered a more comprehensive way to investigate parasite diversity than the usual identification methods and provided new insights whose importance for bumblebee health should be further analysed.

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Section: Resultsmentioning

confidence: 91%

Wide diversity of parasites in Bombus terrestris (Linnaeus, 1758) revealed by a high‐throughput sequencing approach

Bartolomé

Jabal-Uriel

Buendía-Abad

et al. 2020

Environmental Microbiology

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“…Following the "one host-one parasite" paradigm that governed the classification of trypanosomatids for many years, these were both considered as different species. However, a recent taxonomic revision based on DNA barcoding [9] showed the sequences of both these species to be identical, leading to the proposal to unify the nomenclature under the name of the first chronological described species, C. flexonema. However, to the best of our knowledge there has been no consensus on this proposal and most of the bibliography consulted refers to C. acanthocephali.…”

Section: Introductionmentioning

confidence: 99%

“…Crithidia acanthocephali is quite widespread geographically [9], and as previous works have proved, it can proliferate inside insects from different orders, such as Diptera, Coleoptera, or Orthoptera, increasing their mortality [10,11]. Although to date there is little information available regarding this trypanosomatid in honey bees, a recent study detected C. acanthocephali for the first time in honey bee colonies in the center of Spain via Ion PGM sequencing, along with other species commonly found in honey bee colonies (e.g., Lotmaria passim and Crithidia mellificae) [12].…”

Section: Introductionmentioning

confidence: 99%

The Haptomonad Stage of Crithidia acanthocephali in Apis mellifera Hindgut

Buendía-Abad

García-Palencia

Pablos

et al. 2022

Veterinary Sciences

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Crithidia acanthocephali is a trypanosomatid species that was initially described in the digestive tract of Hemiptera. However, this parasite was recently detected in honey bee colonies in Spain, raising the question as to whether bees can act as true hosts for this species. To address this issue, worker bees were experimentally infected with choanomastigotes from the early stationary growth phase and after 12 days, their hindgut was extracted for analysis by light microscopy and TEM. Although no cellular lesions were observed in the honey bee’s tissue, trypanosomatids had differentiated and adopted a haptomonad morphology, transforming their flagella into an attachment pad. This structure allows the protozoa to remain attached to the gut walls via hemidesmosomes-such as junctions. The impact of this species on honey bee health, as well as the pathogenic mechanisms involved, remains unknown. Nevertheless, these results suggest that insect trypanosomatids may have a broader range of hosts than initially thought.

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