Increasing putative vector importance of Trichophoromyia phlebotomines (Diptera: Psychodidae)

Santos, Thiago Vasconcelos dos; Sílveira, Fernando Tobias

doi:10.1590/0074-02760190284

Cited by 14 publications

(6 citation statements)

References 30 publications

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“…auraensis and Th. octavioi are especially important because the first is considered a putative vector of Leishmania (Viannia) in the Amazon region, including the state of Acre [33][34][35], in addition of their females being isomorphic [4], so the male-female association using barcodes may be implemented to correctly identify these insects in upcoming studies (e.g., [15,36,37]). We are providing the first COI barcodes for two vector species-Ny.…”

Section: Plos Onementioning

confidence: 99%

DNA barcoding of sand flies (Diptera, Psychodidae, Phlebotominae) from the western Brazilian Amazon

et al. 2023

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The subfamily Phlebotominae comprises important insects for public health. The use of complementary tools such as molecular taxonomy is necessary for interspecific delimitation and/or discovery of cryptic species. Here, we evaluated the DNA barcoding tool to identify different species in the southwestern Brazilian Amazon. For this, we collected sand flies in forest fragments along the highway BR-317, in the municipality of Brasiléia, state of Acre, Brazil. The specimens were DNA-barcoded using a fragment of the cytochrome c oxidase subunit I (COI) gene. The sequences were analyzed to generate K2P pairwise genetic distances and a Neighbour-joining tree. The sand fly barcodes were also clustered into Molecular Operation Taxonomic Units (MOTU) using Automatic Barcode Gap Discovery (ABGD) approach. A total of 59 COI sequences comprising 22 nominal species and ten genera were generated. Of these, 11 species had not been sequenced before, thus being new COI sequences to science. Intraspecific genetic distances ranged between 0 and 4.9%, with Pintomyia serrana presenting the highest values of genetic distance, in addition to having been partitioned into three MOTUs. Regarding the distances to the nearest neighbour, all species present higher values in relation to the maximum intraspecific distance, in addition to forming well supported clusters in the neighbour-joining analysis. The DNA barcoding approach is useful for the molecular identification of sand flies from Brasiléia, state of Acre, and was efficient in detecting cryptic diversity of five species which can be confirmed in future studies using an integrative approach. We also generated new COI barcodes for Trichophoromyia auraensis, Nyssomyia shawi, and Psychodopygus paraensis, which may play a role in the transmission of Leishmania spp. in the Brazilian Amazon.

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Section: Plos Onementioning

confidence: 99%

DNA barcoding of sand flies (Diptera, Psychodidae, Phlebotominae) from the western Brazilian Amazon

et al. 2023

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“…In addition, other closely related species, such as Trichophoromyia auraensis (Mangabeira), Trichophoromyia brachipyga (Mangabeira), Trichophoromyia ininii (Floch & Abonnenc), Trichophoromyia ruifreitasi Oliveira, Teles, Medeiros, Camargo & Pessoa and Trichophoromyia velascoi (Le Pont & Desjeux) have been suggested as vectors of Leishmania spp. as well (Valdivia et al ., 2012; Brazil et al ., 2015; Teles et al ., 2016; De Araujo‐Pereira et al ., 2017; Dos Santos & Silveira, 2020).…”

Section: Species Complexes and Cryptic Species In New World Phlebotomine Sand Fliesmentioning

confidence: 99%

“…Females belonging to the genus Trichophoromyia Barretto are generally indistinguishable (20 species) (Table 1) or very difficult to identify based on morphology (Galati, 2018; Posada‐López et al ., 2018; Dos Santos & Silveira, 2020); several females of this genus are unknown (Galati, 2018). This group includes Trichophoromyia ubiquitalis (Mangabeira), the main vector of Leishmania lainsoni Silveira, Shaw, Braga & Ishikawa in Pará state, Brazil (Maroli et al ., 2013; Brazil et al ., 2015).…”

Section: Species Complexes and Cryptic Species In New World Phlebotom...mentioning

confidence: 99%

Beyond taxonomy: species complexes in New World phlebotomine sand flies

Sousa‐Paula

Pessoa

Otranto

et al. 2021

Medical Vet Entomology

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A species complex (= species group, species series) is an assemblage of species, which are related morphologically and phylogenetically. Recent research has revealed several arthropod vector species that were believed to be a single nominal species actually representing a group of closely related species, which are sometimes morphologically indistinguishable at one or more developmental stages. In some instances, differences in terms of vector competence, capacity, or both have been recorded. It highlights the importance of detecting and studying species complexes to improve our understanding of pathogen transmission patterns, which may be vectored more or less efficiently by different species within the complex. Considering more than 540 species, about one‐third of the phlebotomine sand flies in the New World present males and/or females morphologically indistinguishable to one or more species. Remarkably, several of these species may act in transmission of pathogenic agents. In this article, we review recent research on species complexes in phlebotomine sand flies from the Americas. Possible practical implications of recently acquired knowledge and future research needs are also discussed.

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“…Ubiquitalis and Th. brachipyga, have been shown to be involved in transmission [18]; ultimately, for L. (V.) lindenbergi, Nyssomyia antunesi has received attention due to its abundance, dominance, spatiotemporal convergence with human disease, blood feeding on human and potential reservoirs of Leishmania [19,20]. Despite the lack of evidence of true and species-specific identifiable Leishmania infection, the vector role of Ny.…”

Section: Introductionmentioning

confidence: 99%

Experimental Susceptibility of Nyssomyia antunesi and Lutzomyia longipalpis (Psychodidae: Phlebotominae) to Leishmania (Viannia) lainsoni and L. (V.) lindenbergi (Trypanosomatidae: Leishmaniinae)

Sánchez Uzcátegui,

Silveira,

de Morais

et al. 2024

Microorganisms

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The present work assessed the experimental susceptibility of Nyssomyia antunesi and Lutzomyia longipalpis to Leishmania (Viannia) lainsoni and L. (V.) lindenbergi. A L. (Leishmania) chagasi–Lu. longipalpis combination was used as a susceptible control. Wild-caught Ny. antunesi and laboratory-bred Lu. longipalpis were membrane-fed on blood with a 5 × 106/mL log-phase promastigote culture suspension and dissected on days 2 and 8 post-blood meal (pbm) for analysis focused on the assessment of parasitoses, as well as placement and promastigote morphotyping. Survival curves were constructed. In all combinations, promastigotes were observed on day 8 pbm. For both Leishmania species, in Lu. longipalpis, the presence of parasites was observed up to the stomodeal valve, while in Ny. antunesi, the presence of parasites was observed up to the cardia. There were no significant differences in parasitosis between L. (V.) lainsoni and L. (V.) lindenbergi in either Ny. antunesi or Lu. longipalpis. Six morphological promastigote forms were distinguished in Giemsa-stained gut smears. The survival curves of all combinations decreased and were affected differently by several Lu. longipalpis–parasite combinations, as well with Lu. longipalpis–uninfected blood. These findings stress Lu. longipalpis as experimentally susceptible to Leishmania spp. and suggest the putative susceptibility of Ny. antunesi to L. (V.) lainsoni and L. (V.) lindenbergi.

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Increasing putative vector importance of Trichophoromyia phlebotomines (Diptera: Psychodidae)

Cited by 14 publications

References 30 publications

DNA barcoding of sand flies (Diptera, Psychodidae, Phlebotominae) from the western Brazilian Amazon

DNA barcoding of sand flies (Diptera, Psychodidae, Phlebotominae) from the western Brazilian Amazon

Beyond taxonomy: species complexes in New World phlebotomine sand flies

Experimental Susceptibility of Nyssomyia antunesi and Lutzomyia longipalpis (Psychodidae: Phlebotominae) to Leishmania (Viannia) lainsoni and L. (V.) lindenbergi (Trypanosomatidae: Leishmaniinae)

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