Genomic basis of antibiotic resistance in Vibrio parahaemolyticus strain JPA1

Coutinho, Felipe H.; Tschoeke, Diogo A.; Clementino, Maysa M.; Thompson, Cristiane C.; Thompson, Fabiano L.

doi:10.1590/0074-02760190053

Cited by 7 publications

(6 citation statements)

References 19 publications

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“…Currently, no metallo-bata-lactamase inhibitors are available, making resistance conferred via metallo-beta-lactamases a difficult challenge to overcome [56] . A study by Coutinho et al determined the presence of CARB-18 gene, which encodes for a beta-lactamase in the studied V. parahaemolyticus strain JPA1 [57] . The presence of the beta lactamase was associated with the strain's resistance towards the beta-lactams such as ampicillin, ampicillin/sulbactam, and ceftazidime [57] .…”

Section: Data Descriptionmentioning

confidence: 99%

“…A study by Coutinho et al determined the presence of CARB-18 gene, which encodes for a beta-lactamase in the studied V. parahaemolyticus strain JPA1 [57] . The presence of the beta lactamase was associated with the strain's resistance towards the beta-lactams such as ampicillin, ampicillin/sulbactam, and ceftazidime [57] . These findings are similar to those from the phenotype and genome of RP0132.…”

Section: Data Descriptionmentioning

confidence: 99%

“…The CAT enzymes inactivate the drug via acetylation to ensure bacteria survival [58,59] . ARGs encoding for CAT have been found in V. parahaemolyticus and members of the Vibrio family, such as V. cholerae and V. vulnificus are known to cause human diseases [57,60] . Moreover, multiple genes encoding for efflux pumps were also found within the genome, indicating other possible mechanisms of drug efflux contributing to its antibiotic resistance (Table 2).…”

Section: Data Descriptionmentioning

confidence: 99%

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Complete Whole Genome Sequence of Vibrio parahaemolyticus RP0132 Strain Isolated from Shrimp in Malaysia

Loo,

Tan,

Law

et al. 2023

Prog Micobes Mol Biol

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Vibrio parahaemolyticus is a Gram-negative, halophilic bacterium that is ubiquitous in marine environments. Its perilous co-existence with aquatic animals increases the risk of infections and diseases, especially those in aquaculture systems, thus resulting in reduced production and economic losses in the aquaculture industry. Moreover, V. parahaemolyticus can be easily transmitted to humans via consumption of contaminated seafood, resulting in gastroenteritis outbreaks. However, the rise in multidrug resistance within the species has challenged the efficacy of antimicrobial treatments against V. parahaemolyticus infections. Therefore, we report the genome sequence of V. parahaemolyticus RP0132 isolated from shrimp to gain insight into its antimicrobial resistance traits and potential resistance mechanisms. The findings will facilitate the development of effective anti-Vibrio agents to manage V. parahaemolyticus infections.

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Section: Data Descriptionmentioning

confidence: 99%

Section: Data Descriptionmentioning

confidence: 99%

Section: Data Descriptionmentioning

confidence: 99%

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Complete Whole Genome Sequence of Vibrio parahaemolyticus RP0132 Strain Isolated from Shrimp in Malaysia

Loo,

Tan,

Law

et al. 2023

Prog Micobes Mol Biol

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“…From the waters of the Jacarepaguá lagoon system, in Rio de Janeiro, Brazil, a MDR V. parahaemolyticus strain JPA1 was isolated. The strain was tested against 16 antibiotics and displayed resistance to eight of them, seven β-lactams and one a polymyxin, but was susceptible to all aminoglycosides, tigecycline, and ciprofloxacin [173]. Despite the V. parahaemolyticus strain JPA1 being non-virulent to humans, it encodes several genes that have been involved in several mechanisms of resistance against different classes of antibiotics, such as antibiotic efflux, regulation of efflux, antibiotic inactivation, and target replacement [173].…”

Section: Antibiotic Susceptibility In Vibrio Sppmentioning

confidence: 99%

Vibrio spp.: Life Strategies, Ecology, and Risks in a Changing Environment

et al. 2022

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Vibrios are ubiquitous bacteria in aquatic systems, especially marine ones, and belong to the Gammaproteobacteria class, the most diverse class of Gram-negative bacteria. The main objective of this review is to update the information regarding the ecology of Vibrio species, and contribute to the discussion of their potential risk in a changing environment. As heterotrophic organisms, Vibrio spp. live freely in aquatic environments, from marine depths to the surface of the water column, and frequently may be associated with micro- and macroalgae, invertebrates, and vertebrates such as fish, or live in symbiosis. Some Vibrio spp. are pathogenic to humans and animals, and there is evidence that infections caused by vibrios are increasing in the world. This rise may be related to global changes in human behavior (increases in tourism, maritime traffic, consumption of seafood, aquaculture production, water demand, pollution), and temperature. Most likely in the future, Vibrio spp. in water and in seafood will be monitored in order to safeguard human and animal health. Regulators of the microbiological quality of water (marine and freshwater) and food for human and animal consumption, professionals involved in marine and freshwater production chains, consumers and users of aquatic resources, and health professionals will be challenged to anticipate and mitigate new risks.

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“…cholerae . − Patients with vibriosis are clinically treated using antibiotics, including quinolones, cephalosporins, tetracyclines, and penicillins, with penicillins having the weakest effect . Penicillin-resistant V. parahemolyticus reportedly resists β-lactam antibiotics through the action of certain genes, including acrE, crp , hns, bla CARB‑17 , and bla CYM‑2 , that encode efflux pumps or β-lactamase. The metallo-β-lactamase Vmh, identified in Vibrio vulnificus , exhibited broad degradation activity against β-lactam antibiotics, including penicillins, cephalosporins, and imipenems .…”

Section: Introductionmentioning

confidence: 99%

Characterization and Potentiating Effects of the Ethanolic Extracts of the Red Seaweed Gracillaria sp. on the Activity of Carbenicillin against Vibrios

Tsui

Chang

et al. 2022

ACS Omega

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β-lactam-resistant Vibrio strains are a significant clinical problem, and β-lactamase inhibitors are generally coadministered with β-lactam drugs to control drug-resistant bacteria. Seaweed is a rich source of natural bioactive compounds; however, their potential as β-lactamase inhibitors against bacterial pathogens remains unknown. Herein, we evaluated the potential β-lactamase inhibitory effect of the ethanolic extracts of the red seaweed Gracilaria sp. (GE) against four Vibrio strains. The minimum inhibitory concentration, half-maximal inhibitory concentration, checkerboard assay results, and time-kill study results indicate that GE has limited antibacterial activity but can potentiate the activity of the β-lactam antibiotic carbenicillin against Vibrio parahemolyticus and V. cholerae. We overexpressed and purified recombinant metallo-β-lactamase, VarG, from V. cholerae for in vitro studies and observed that adding GE reduced the carbenicillin and nitrocefin degradation by VarG by 20% and 60%, respectively. Angiotensin I-converting enzyme inhibition studies demonstrated that GE did not inhibit VarG via metal chelation. Toxicity assays indicated that GE exhibited mild toxicity against human cells. Through gas chromatography and mass spectrometry, we showed that GE comprises alkaloids, phenolic compounds, terpenoids, terpenes, and halogenated aromatic compounds. This study revealed that extracts of the red seaweed Gracillaria sp. can potentially inhibit β-lactamase activity.

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Genomic basis of antibiotic resistance in Vibrio parahaemolyticus strain JPA1

Cited by 7 publications

References 19 publications

Complete Whole Genome Sequence of Vibrio parahaemolyticus RP0132 Strain Isolated from Shrimp in Malaysia

Complete Whole Genome Sequence of Vibrio parahaemolyticus RP0132 Strain Isolated from Shrimp in Malaysia

Vibrio spp.: Life Strategies, Ecology, and Risks in a Changing Environment

Characterization and Potentiating Effects of the Ethanolic Extracts of the Red Seaweed Gracillaria sp. on the Activity of Carbenicillin against Vibrios

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