Genome sequence of a multidrug-resistant Corynebacterium striatum isolated from bloodstream infection from a nosocomial outbreak in Rio de Janeiro, Brazil

Ramos, Juliana Nunes; Rodrigues, Izabel dos Santos; Baio, Paulo Victor Pereira; Veras, João Flávio Carneiro; Ramos, Rommel Thiago Jucá; Pacheco, Luis Gustavo Carvalho; Azevedo, Vasco; Júnior, Raphael Hirata; Abanto, Michel; Mattos‐Guaraldi, Ana Luíza; Vieira, Verônica Viana

doi:10.1590/0074-02760180051

Cited by 15 publications

(15 citation statements)

References 22 publications

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“…Genomes include complete, draft, and raw data genomes from the USA, China, Brazil, Japan, and, more recently, genomes from Germany and Denmark. The genome length is ~2.8 Mb, and the GC content is around 59% [ 34 ]. Genomic data of C. striatum from NCBI shows three plasmids in C. striatum , pCs-Na-1, pCs-Na-2, and pTP10.…”

Section: Genome Organization Of C Striatummentioning

confidence: 99%

“…Genomic data of C. striatum from NCBI shows three plasmids in C. striatum , pCs-Na-1, pCs-Na-2, and pTP10. The pTP10 plasmid has been reported in C. striatum M82B [ 35 ] and its presence in other genomes been studied [ 34 ]. The presence of viral genomes in the C. striatum has not been adequately studied, but recently a study identified a phage species from the Siphoviridae family in C. striatum [ 36 ].…”

Section: Genome Organization Of C Striatummentioning

confidence: 99%

“…Fluoroquinolone resistance mechanisms include the alteration of membrane permeability, efflux, inactivation by acetylation and, the most common mechanism, mutations at the site of action of the antibiotic in topoisomerases [ 37 , 38 ]. In C. striatum , several studies have described mutations in the quinolone resistance-determining regions (QRDR) in the gyrA gene [ 12 , 16 , 17 , 34 , 45 , 46 , 47 ]. Sierra and co-workers (2005) [ 46 ] reported mutations for positions 87 and 91 in the peptide sequence of GyrA.…”

Section: C Striatum Resistomementioning

confidence: 99%

“…Sierra and co-workers (2005) [ 46 ] reported mutations for positions 87 and 91 in the peptide sequence of GyrA. Later investigations performed by Ramos and co-workers (2018 and 2020) [ 34 , 45 ] showed a preference of C. striatum isolates to mutate from Ser87Val and less often from Asp91Asn or Asp91Ala. Alibi et al (2017) [ 47 ] reported mutations of Ser87Phe and Asp91Ala or Asp91Gly, while Drogomirescu et al (2020) [ 20 ] showed that C. striatum strains increased their resistance to fluoroquinolones when the mutation occurred from Ser87Phe and Asp91Ala.…”

Section: C Striatum Resistomementioning

confidence: 99%

“…Whole-genome sequencing (WGS) has been an essential tool for understanding molecular epidemiology, global transmission, and virulence mechanisms of pathogens. Although WGS has been used for C. striatum investigation of genomic features of MDR and non-MDR clinical isolates, further studies remain necessary to study strain-dependent genomic elements related to the acquisition of resistance to antimicrobial agents [ 16 , 32 , 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

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Treat Me Well or Will Resist: Uptake of Mobile Genetic Elements Determine the Resistome of Corynebacterium striatum

Leyton-Carcaman

Ramos

Baio

et al. 2021

IJMS

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Corynebacterium striatum, a bacterium that is part of the normal skin microbiota, is also an opportunistic pathogen. In recent years, reports of infections and in-hospital and nosocomial outbreaks caused by antimicrobial multidrug-resistant C. striatum strains have been increasing worldwide. However, there are no studies about the genomic determinants related to antimicrobial resistance in C. striatum. This review updates global information related to antimicrobial resistance found in C. striatum and highlights the essential genomic aspects in its persistence and dissemination. The resistome of C. striatum comprises chromosomal and acquired elements. Resistance to fluoroquinolones and daptomycin are due to mutations in chromosomal genes. Conversely, resistance to macrolides, tetracyclines, phenicols, beta-lactams, and aminoglycosides are associated with mobile genomic elements such as plasmids and transposons. The presence and diversity of insertion sequences suggest an essential role in the expression of antimicrobial resistance genes (ARGs) in genomic rearrangements and their potential to transfer these elements to other pathogens. The present study underlines that the resistome of C. striatum is dynamic; it is in evident expansion and could be acting as a reservoir for ARGs.

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Section: Genome Organization Of C Striatummentioning

confidence: 99%

Section: Genome Organization Of C Striatummentioning

confidence: 99%

Section: C Striatum Resistomementioning

confidence: 99%

Section: C Striatum Resistomementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Treat Me Well or Will Resist: Uptake of Mobile Genetic Elements Determine the Resistome of Corynebacterium striatum

Leyton-Carcaman

Ramos

Baio

et al. 2021

IJMS

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Antibiotic resistance genes in the Actinobacteria phylum

Bafghi

2019

Eur J Clin Microbiol Infect Dis

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The pan-genome of the emerging multidrug-resistant pathogen Corynebacterium striatum

Jesus

Ramos

Rocha

et al. 2022

Funct Integr Genomics

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Corynebacterium striatum, a common constituent of the human skin microbiome, is now considered an emerging multidrug-resistant pathogen of immunocompromised and chronically ill patients. However, little is known about the molecular mechanisms in the transition from colonization to the multidrugresistant (MDR) invasive phenotype in clinical isolates. This study performed a comprehensive pangenomic analysis of C. striatum, including isolates from 'normal skin microbiome' and from MDR infections, to gain insights into genetic factors contributing to pathogenicity and multidrug resistance in this species. For this, three novel genome sequences were obtained from clinical isolates of C. striatum of patients from Brazil, and other 24 complete or draft C. striatum genomes were retrieved from GenBank, including the ATCC6940 isolate from the Human Microbiome Project. Analysis of C. striatum strains demonstrated the presence of an open pan-genome (α = 0.852803) containing 3,816 gene families, including 15 antimicrobial resistance (AMR) genes and 32 putative virulence factors. The core and accessory genomes included 1,297 and 1,307 genes, respectively. The identi ed AMR genes are primarily associated with resistance to aminoglycosides and tetracyclines. Of these, 66.6% are present in genomic islands, and four AMR genes, including aac(6')-ib7, are located in a class 1-integron. In conclusion, our data indicated that C. striatum possesses genomic characteristics favorable to the invasive phenotype, with high genomic plasticity, a robust genetic arsenal for iron acquisition, and important virulence determinants and AMR genes present in mobile genetic elements.

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Genome sequence of a multidrug-resistant Corynebacterium striatum isolated from bloodstream infection from a nosocomial outbreak in Rio de Janeiro, Brazil

Cited by 15 publications

References 22 publications

Treat Me Well or Will Resist: Uptake of Mobile Genetic Elements Determine the Resistome of Corynebacterium striatum

Treat Me Well or Will Resist: Uptake of Mobile Genetic Elements Determine the Resistome of Corynebacterium striatum

Antibiotic resistance genes in the Actinobacteria phylum

The pan-genome of the emerging multidrug-resistant pathogen Corynebacterium striatum

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