Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery

Costa, Jimena Ferreira da; Ferrarini, Mariana Galvão; Nardelli, Sheila Cristina; Goldenberg, Samuel; Ávila, Andréa Rodrigues; Holetz, Fabíola

doi:10.1590/0074-02760170531

Cited by 6 publications

(2 citation statements)

References 30 publications

(57 reference statements)

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“…TcSgn1 localization was, in fact, altered in the presence of sinefungin, resulting in its distribution at the periphery of the nucleus and weak staining in the cytoplasm. A similar alteration was also shown for the exonuclease XRNA and RNA helicase DHH1 [ 36 ]. With sinefungin, both proteins colocalized at the nuclear periphery.…”

Section: Discussionsupporting

confidence: 74%

Characterization of the RNA-Binding Protein TcSgn1 in Trypanosoma cruzi

et al. 2021

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RNA-binding proteins (RBPs) participate in several steps of post-transcriptional regulation of gene expression, such as splicing, messenger RNA transport, mRNA localization, and translation. Gene-expression regulation in trypanosomatids occurs primarily at the post-transcriptional level, and RBPs play important roles in the process. Here, we characterized the RBP TcSgn1, which contains one RNA recognition motif (RRM). TcSgn1 is a close ortholog of yeast Saccharomyces cerevisiae protein ScSgn1, which plays a role in translational regulation in the cytoplasm. We found that TcSgn1 in Trypanosoma cruzi is localized in the nucleus in exponentially growing epimastigotes. By performing immunoprecipitation assays of TcSgn1, we identified hundreds of mRNAs associated with the protein, a significant fraction of them coding for nucleic acids binding, transcription, and endocytosis proteins. In addition, we show that TcSgn1 is capable of interacting directly with the poly(A) tail of the mRNAs. The study of parasites under nutritional stress showed that TcSgn1 was localized in cytoplasmic granules in addition to localizing in the nucleus. Similar to ScSgn1, we observed that TcSgn1 also interacts with the PABP1 protein, suggesting that this protein may play a role in regulating gene expression in T. cruzi. Taken together, our results show that RNA-binding protein TcSgn1 is part of ribonucleoprotein complexes associated with nuclear functions, stress response, and RNA metabolism.

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Section: Discussionsupporting

confidence: 74%

Characterization of the RNA-Binding Protein TcSgn1 in Trypanosoma cruzi

et al. 2021

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“…The RNP granules described in T. cruzi [ 104 , 105 ] contain similar components to yeast or mammalian processing bodies (P-bodies), e.g., TcDHH1, and TcXRNA proteins. These proteins are expressed through the parasite life cycle and are localized in cytoplasmic foci that vary in number according to nutritional stress conditions and cycloheximide/puromycin treatment [ 106 , 107 ]. TcDHH1 and TcXRNA co-localize only at the nuclear periphery under cellular stress conditions and when the mRNA processing is inhibited [ 107 ].…”

Section: The Importance Of Rnp Granules In Modulating Gene Expression...mentioning

confidence: 99%

Modulation of Virulence Factors during Trypanosoma cruzi Differentiation

et al. 2022

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Chagas disease is a neglected tropical disease caused by Trypanosoma cruzi. This protozoan developed several mechanisms to infect, propagate, and survive in different hosts. The specific expression of proteins is responsible for morphological and metabolic changes in different parasite stages along the parasite life cycle. The virulence strategies at the cellular and molecular levels consist of molecules responsible for mediating resistance mechanisms to oxidative damage, cellular invasion, and immune evasion, performed mainly by surface proteins. Since parasite surface coat remodeling is crucial to invasion and infectivity, surface proteins are essential virulence elements. Understanding the factors involved in these processes improves the knowledge of parasite pathogenesis. Genome sequencing has opened the door to high-throughput technologies, allowing us to obtain a deeper understanding of gene reprogramming along the parasite life cycle and identify critical molecules for survival. This review therefore focuses on proteins regulated during differentiation into infective forms considered virulence factors and addresses the current known mechanisms acting in the modulation of gene expression, emphasizing mRNA signals, regulatory factors, and protein complexes.

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