Draft genome of the emerging pathogen, Kocuria marina, isolated from a wild urban rat

Loong, Shih Keng; Tan, Kian Lam; Zainal, Nurhafiza; Phoon, Wai Hong; Zain, Siti Nursheena Mohd; AbuBakar, Sazaly

doi:10.1590/0074-02760170132

Cited by 5 publications

(3 citation statements)

References 14 publications

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“…Visual inspection of the bacterial isolate after incubation in a 96-well plate at 37°C for 48 h [10] noted that the C. lipophiloflavum isolate did not produce biofilm. Morphological characteristics of the bacterium unveiled by transmission electron microscopy [11] displayed structures similar to C. phoceense [12] and C. lactis [13] [Figure . 2]. The bacterium showed characteristic clubshaped cell and the presence of an external lipid layer.…”

Section: Resultsmentioning

confidence: 99%

Microbiological analysis of raw milk unveiled the presence of a dairy contaminant, Corynebacterium lipophiloflavum

2019

Jabb

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Dairy farming occupied a distinct position in agriculture since milk can be harvested every day, providing a regular source of income to the farmers. Development of the Malaysian dairy farming industry was marred by poor farm hygiene practices, leading to the proliferation of dairy-spoilage bacteria, affecting milk quality. In this study, we report the isolation and characterization of a rare Corynebacterium species from raw milk after the implementation of improved farm hygiene practices. All milking equipment, farm worker's hands and the cow's udders and teats were washed with detergent and wiped dry with clean towels before milk sample collection. Collected foremilk samples from mastitis-free cows were inoculated onto Petrifilm™ and cultured colonies were plated onto nutrient agar. Biochemical and molecular tests were performed for the identification of peculiar bacterial isolates. A unique yellow-pigmented bacteria isolate was recovered from the milk of a healthy cow after the adoption of improved farm hygiene practices. Phenotypic and genotypic characterization confirmed the milk isolate as Corynebacterium lipophiloflavum. This is the first description of C. lipophiloflavum in cow's milk and could possibly imply the influence of bovine flora in dairy contamination. The findings highlight the increasing spectrum of Corynebacterium species with potential adverse impact to the dairy industry. It is recommended to screen for C. lipophiloflavum in all milk processing facility to ensure that milk is safe for consumption and its products prepared to the highest quality and safety standards.

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Section: Resultsmentioning

confidence: 99%

Microbiological analysis of raw milk unveiled the presence of a dairy contaminant, Corynebacterium lipophiloflavum

2019

Jabb

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“…Transmission electron microscopy performed following the protocols described by Loong et al (2017) [13] revealed morphological similarity of S. cuniculi UM-25 to S. azizii, a phylogenetically close relative found to be associated with meningoencephalitis in C57BL/6 laboratory mice [5]. The diameter of a S. cuniculi cell was approximately 700 nm and surrounded by protein fibrils (Fig.…”

Section: -284 2021mentioning

confidence: 99%

Isolation of <i>Streptococcus cuniculi</i> from corneal lesion in laboratory-raised mice

Tiong

Loong

Wali

et al. 2021

The Journal of Veterinary Medical Science

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Corneal lesions appearing as white mass beneath intact epithelium, with ocular discharge in one mouse, was observed in a batch of laboratory-raised BALB/c mice (n=9 of 56). The affected mice remained active, well-groomed and had normal appetite. Isolates recovered from swab cultures of the external and internal contents of the eye had partial 16S rRNA gene sequence of 99.1% similarity to Streptococcus cuniculi. No previous report of S. cuniculi infection in laboratory rodents has been presented. The isolate was susceptible to all antibiotics tested. We suggest S. cuniculi is an opportunistic bacteria in laboratory mice but are uncertain of its source. Our findings revealed that S. cuniculi is able to colonize laboratory mice and should be considered when mice present with eye lesion or ocular discharge.

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“…Genetic analysis is essential in successfully addressing emerging infectious diseases. 10 , 11 Although a few R. planticola genome sequences are available, the genomic background of its pathogenesis and resistance is largely unknown. Here, we sequenced and reconstructed the complete circular genome of the R. planticola strain GODA and performed genome-wide comparisons in order to decipher the putative virulence and resistance determinants.…”

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confidence: 99%

Comparative genomics reveals diverse capsular polysaccharide synthesis gene clusters in emerging Raoultella planticola

Huang

Chuang

et al. 2018

Mem. Inst. Oswaldo Cruz

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Raoultella planticola is an emerging zoonotic pathogen that is associated with rare but life-threatening cases of bacteremia, biliary tract infections, and urinary tract infections. Moreover, increasing antimicrobial resistance in the organism poses a potential threat to public health. In spite of its importance as a human pathogen, the genome of R. planticola remains largely unexplored and little is known about its virulence factors. Although lipopolysaccharides has been detected in R. planticola and implicated in the virulence in earlier studies, the genetic background is unknown. Here, we report the complete genome and comparative analysis of the multidrug-resistant clinical isolate R. planticola GODA. The complete genome sequence of R. planticola GODA was sequenced using single-molecule real-time DNA sequencing. Comparative genomic analysis reveals distinct capsular polysaccharide synthesis gene clusters in R. planticola GODA. In addition, we found bla TEM-57 and multiple transporters related to multidrug resistance. The availability of genomic data in open databases of this emerging zoonotic pathogen, in tandem with our comparative study, provides better understanding of R. planticola and the basis for future work.

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Draft genome of the emerging pathogen, Kocuria marina, isolated from a wild urban rat

Cited by 5 publications

References 14 publications

Microbiological analysis of raw milk unveiled the presence of a dairy contaminant, Corynebacterium lipophiloflavum

Microbiological analysis of raw milk unveiled the presence of a dairy contaminant, Corynebacterium lipophiloflavum

Isolation of <i>Streptococcus cuniculi</i> from corneal lesion in laboratory-raised mice

Comparative genomics reveals diverse capsular polysaccharide synthesis gene clusters in emerging Raoultella planticola

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