Characterisation of complete high- and low-risk human papillomavirus genomes isolated from cervical specimens in southern Brazil

Oliveira, Gisele Rodrigues de Carvalho; Siqueira, Juliana D.; Finger‐Jardim, Fabiana; Vieira, Valdimara Corrêa; Silva, Ronald L; Gonçalves, Carla Vitola; Soares, Esmeralda A.; Martínez, Ana Maria Barral de; Soares, Marcelo A.

doi:10.1590/0074-02760170121

Cited by 9 publications

(5 citation statements)

References 20 publications

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“…The genomes sequenced in the current study were compared to the 47 complete HPV18 genomes described previously [ 14 , 15 , 16 , 30 , 31 ] and currently available in NCBI Genbank. The present study adds 51 new unique sequences ( Figure 2 ).…”

Section: Resultsmentioning

confidence: 99%

High Whole-Genome Sequence Diversity of Human Papillomavirus Type 18 Isolates

Weele

Meijer

King

2018

Viruses

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Background: The most commonly found human papillomavirus (HPV) types in cervical cancer are HPV16 and HPV18. Genome variants of these types have been associated with differential carcinogenic potential. To date, only a handful of studies have described HPV18 whole genome sequencing results. Here we describe HPV18 variant diversity and conservation of persistent infections in a longitudinal retrospective cohort study. Methods: Cervical self-samples were obtained annually over four years and genotyped on the SPF10-DEIA-LiPA25 platform. Clearing and persistent HPV18 positive infections were selected, amplified in two overlapping fragments, and sequenced using 32 sequence primers. Results: Complete viral genomes were obtained from 25 participants with persistent and 26 participants with clearing HPV18 infections, resulting in 52 unique HPV18 genomes. Sublineage A3 was predominant in this population. The consensus viral genome was completely conserved over time in persistent infections, with one exception, where different HPV18 variants were identified in follow-up samples. Conclusions: This study identified a diverse set of HPV18 variants. In persistent infections, the consensus viral genome is conserved. The identification of only one HPV18 infection with different major variants in follow-up implies that this is a potentially rare event. This dataset adds 52 HPV18 genome variants to Genbank, more than doubling the currently available HPV18 information resource, and all but one variant are unique additions.

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Section: Resultsmentioning

confidence: 99%

High Whole-Genome Sequence Diversity of Human Papillomavirus Type 18 Isolates

Weele

Meijer

King

2018

Viruses

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“…Regarding the number, it ranged from 3 to 34 with 14.20 ± 8.18 as a mean ±SD and 12.50 as a median value. Severity of STs varies from mild (1-10), moderate (11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30) and severe (>30), with (3, 7.5%), (23, 57.5%) (14, 35%) of the patients had severe, moderate and mild STs respectively.…”

Section: Resultsmentioning

confidence: 99%

Low risk and High risk HPV Genotypes in Skin Tags

Elaskary

Haggag

Farag

2019

Egyptian Journal of Medical Microbiology

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Background: Skin tags are flesh-coloured, pedunculated growths with a smooth surface, reported to have an incidence of 46% of the general population. The etiology of skin tags is still unknown, human papillomavirus skin infection is one of the associated factor. Human papillomavirus (HPV) is a small DNA virus of papovavirus family that classified into low risk HPV (6& 11) and high risk HPV (16&18) that induce malignant transformation. presence of HPV in the tissues depends on the identification of viral nucleic acids by PCR than serological methods that can't distinguish between present and past infection of HPV. Objectives: To detect the relationship between human papillomavirus skin infection and skin tags and to asses the relation between low risk and high risk HPV genotypes and skin tags clinical criteria and associated clinical conditions. Methodology:This study was conducted on 40 patients (20 females and 20 males) having skin tags (group I) and 20 sex and age matched subjects free from skin tags (group II). Skin biopsy was taken from each subject and Multiplex Polymerase chain reaction (PCR) was used to detect HPV types 6, 11, 16 and 18 DNA. Results: Family history was statistically significant higher in group I than group II (P=0.002). STs distributions were higher in neck (21, 52.5%) then axilla (11, 27.5%), back (4, 10%), abdomen (2, 5%) and the thigh(2, 5%). A significant difference in HPV positivity between the studied groups, that showed stepwise down regulation from lesional (19, 47.5%) passing by perilesional (5, 12.5%) and ended by normal skin (2, 10%) specimens (P<0.001). Moreover HPV genotype 6 was the most observed type. Conclusion: Human papillomavirus has a role in the pathogenesis of skin tags. Therefore using of antiviral or immunotherapy may help in skin tags management program. There is no relation between HPV genotypes and skin tags clinical criteria (sites & severity) nor associated clinical conditions. METHODOLOGYThis case-control study was conducted on patients (n=40) having variable numbers of skin tags lesions. They were compared with age and sex-matched healthy volunteers (n=20) as a control group. Patients were recruited from the Outpatient Clinic of Dermatology,

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“…En nuestro estudio no se encontró significancia estadística entre la infección por virus de papiloma humano y el número de partos vaginales, similar a lo hallado por Rajkumar y col. (33) . Tampoco se halló significancia estadística entre el haber tenido alguna lesión de cuello uterino y la infección por VPH, lo que difiere de estudios (31) , Oliveira (34) , Motoyama (35) , Naqvi, Wajid y Mitra (36) y Harden y Munger (13) . Los antecedentes de ITS, uso de anticonceptivo hormonal y/o condón y fumar no mostraron significancia estadística con el resultado positivo por VPH; esto difiere con los estudios de Hellberg y de Vaccarella, que encuentran relación entre estos factores y la infección por VPH (23,37,38) .…”

Section: Discussionunclassified

Virus papiloma humano y factores asociados en pacientes con citología desconocida atendidas en el norte de Perú

Iglesias-Osores¹,

Serquén-López²

2020

Rev peru ginecol obstet.

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Introducción. El virus papiloma humano es el causante del cáncer de cuello uterino, uno de los cánceres más comunes en las mujeres. Objetivo. Determinar la prevalencia del virus papiloma humano y los factores asociados en mujeres con citología desconocida. Métodos. En pacientes de ginecoobstetricia con citología desconocida atendidas en el Hospital Regional Lambayeque, en la costa norte del Perú, entre abril y junio 2019, se realizó la extracción de ADN para identificar el virus del papiloma humano basada en el método de salting out. Se procesaron las muestras mediante reacción en cadena de la polimerasa y se amplificaron para los primers MY09 y MY11, y PC04/GH20. Se empleó el análisis bivariado mediante la prueba de chi cuadrado y t-student. Resultados. Se encontró que 29,9% de las pacientes atendidas en el área de gineco-obstetricia con citología desconocida tuvieron el virus del papiloma humano. No se encontró diferencia estadística significativa entre la infección por virus del papiloma humano con la edad, edad de primera relación sexual, promiscuidad, número de partos vaginales, lesión de cuello uterino, antecedente de ITS, uso de anticonceptivo hormonal, uso del condón y tabaquismo.

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Characterisation of complete high- and low-risk human papillomavirus genomes isolated from cervical specimens in southern Brazil

Cited by 9 publications

References 20 publications

High Whole-Genome Sequence Diversity of Human Papillomavirus Type 18 Isolates

High Whole-Genome Sequence Diversity of Human Papillomavirus Type 18 Isolates

Low risk and High risk HPV Genotypes in Skin Tags

Virus papiloma humano y factores asociados en pacientes con citología desconocida atendidas en el norte de Perú

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