Comparison of four molecular approaches to identify Candida parapsilosis complex species

Barbedo, Leonardo Silva; Figueiredo-Carvalho, Maria Helena Galdino; Muniz, Mauro de Medeiros; Zancopé‐Oliveira, Rosely Maria

doi:10.1590/0074-02760160412

Cited by 4 publications

(5 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Hass et al applied the matrix assisted laser desorption ionization-time of flight mass spectrometry method for the discrimination of these species (20). Moreover, Barbedo et al reported agreement in the results of four PCR-based methods (i.e., species-specific uniplex PCR, sequencing of the D1/D2 region of the LSU 28S rDNA gene, microsatellite typing, and PCR-RFLP of ITS) for species identification among 98 clinical isolates (5).…”

Section: Discussionmentioning

confidence: 99%

“…It is considered as a nosocomial yeast pathogen that causes a high variety of clinical manifestations such as endocarditis, fungemia, septic arthritis, pancreatitis, endophthalmitis, and meningitis (2). Since the early 1990s, based on a variety of molecular studies such as randomly amplified polymorphic DNA analysis (RAPD) (3), mitochondrial DNA sequences analysis (4), and internal transcribed spacer (ITS) sequencing (5), C. parapsilosis has been categorized as a complex consisting of three genetically distinct groups (named I, II and III). Tavanti et al replaced the groups II and III with C. orthopsilosis and C.metapsilosis based on multilocus sequence typing (MLST) data and identified three separate species as the C. parapsilosis complex (6).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Discrimination of the Candida parapsilosis Species Complex via SADH Gene Analysis and Evaluation of Proteinase Activity Among the Isolates

Pakshir

Karimi

Zomorodian

et al. 2018

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background: Candida parapsilosis consists of three species of C. parapsilosis sensu stricto, C. metapsilosis, and C. orthopsilosis. Proteinase activity is considered as one of the major virulence factors in the pathogenicity of Candida species. Objectives: We aimed to discriminate clinical isolates of the C. parapsilosis species complex through analysis of the secondary alcohol dehydrogenase (SADH) gene using BanI restriction enzyme and evaluation of proteinase activity among the isolates. Methods: In this study, 71 clinical isolates identified as C. parapsilosis were included. The species were discriminated via analysis of SADH gene using S1F and S1R primers. The polymerase chain reaction (PCR) products were digested with restriction enzyme BanI. Proteinase activity of the species was evaluated using bovine serum albumin agar medium. To analyze the data, Fisher's exact test was used. Results: The results presented that out of the 71 species tested, 65 (91.5%) were C. parapsilosis sensu stricto, 6 (8%) were C. orthopsilosis, and no species were C. metapsilosis. Proteinase activity was observed in 70.1% and 80.3% of C. parapsilosis and C. orthopsilosis species, respectively. Conclusions: In this study, the most dominant species identified by the molecular method was C. parapsilosis sensu stricto. The high rate of proteinase activity among the isolates indicates the importance of this enzyme as a virulence factor in the pathogenicity of this complex.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Discrimination of the Candida parapsilosis Species Complex via SADH Gene Analysis and Evaluation of Proteinase Activity Among the Isolates

Pakshir

Karimi

Zomorodian

et al. 2018

Jundishapur J Microbiol

View full text Add to dashboard Cite

show abstract

“…The sensitivity of the detection molecular methods depends on various factors, one of the most important of which is the selection of the target DNA for amplification. Over the last two decades, the use of molecular diagnostic study for the detection of a specific sequence of Candida to investigate intra-species and inter-species differences has been reviewed [18,19]. In such studies, when the main purpose was to design pan-Candida primers, the designed primer pair usually amplified common environmental contaminants, such as Aspergillus.…”

Section: Discussionmentioning

confidence: 99%

Translation elongation factor 1-alpha gene as a marker for diagnosing of Candida onychomycosis

Pakshir¹,

Farazmand²,

Ghasemi³

et al. 2020

CMM

View full text Add to dashboard Cite

Background and Purpose: Culture-based identification methods have been the gold standard for the diagnosis of candidal onychomycosis. Molecular technologies, such as polymerase chain reaction (PCR) assays, can provide an alternative for the rapid detection of Candida species. The present study was conducted to investigate a pan-Candida PCR assay based on the translation elongation factor 1-alpha (TEF-1α) gene for the detection of the most prevalent pathogenic Candida species. Materials and Methods: For the purpose of the study, an optimized pan-Candida PCR primer pair was designed, and the target was amplified and sequenced. The analytical and clinical diagnostic performance of the designed primers was tested using 17 reference strains, 137 nail scrapings suspected of onychomycosis, and 10 healthy nail specimens. Results: The use of the universal pan-Candida primers designed on TEF-1α gene resulted in the successful amplification of a 270-base pair fragment in all Candida species tested, except for C. glabrata, and reacted neither with other fungi nor with E. coli. The sequence difference count matrix showed poor insertion/deletion differences (0-2 nt) among Candida species. Among 137 nail specimens, 35% (n=48), 30.7% (n=42), and 40.1% (n=55) of the samples were found to be positive by direct microscopy, culture, and pan-Candida PCR, respectively. Conclusion: Based on the findings, the PCR-based detection targeting the DNA TEF-1α gene is a rapid and simple procedure for the diagnosis of candidal onychomycosis directly from nail sample.

show abstract

“…The internal transcription spacer (ITS) of the rDNA locus has been acknowledged as the worldwide gold standard for fungal species identification [24,25], and various global primers amplify this region. Other parts of the rDNA locus, such as Trichosporum's Intergenic Spacer region 1 (IGS1) may be more useful for identifying certain clusters or species [26].…”

Section: End-point Pcr-based Amplificationmentioning

confidence: 99%

Molecular Detection and Identification of Candida

Al-Taee¹

2023

Candida and Candidiasis

View full text Add to dashboard Cite

Human opportunistic yeast infections have become more common in recent years. Many infections are difficult to treat and diagnose due to the large number and diversity of organisms that can cause sickness. In addition, infectious strains eventually develop resistance to one or more antifungal medicines, severely limiting treatment choices and emphasizing the need of early detection of the infective agent and its drug sensitivity profile. Current techniques for detecting species and resistances are insensitive and specific, and they frequently need pre-cultivation of the causal agent, which delays diagnosis. New high-throughput technologies, such as next-generation sequencing or proteomics, make it possible to identify yeast infections more sensitively, accurately, and quickly. Opportunistic yeast pathogens, cause a wide spectrum of superficial and systemic infections, many of which are lethal. In this work, we give an overview of current and newly created approaches. It may be used to determine the presence of yeast infections as well as their medication resistance. Throughout the book, we highlight the following points: Explaining the benefits and drawbacks of each strategy, as well as the most promising advancements on their route to success.

show abstract

Comparison of four molecular approaches to identify Candida parapsilosis complex species

Cited by 4 publications

References 30 publications

Molecular Discrimination of the Candida parapsilosis Species Complex via SADH Gene Analysis and Evaluation of Proteinase Activity Among the Isolates

Molecular Discrimination of the Candida parapsilosis Species Complex via SADH Gene Analysis and Evaluation of Proteinase Activity Among the Isolates

Translation elongation factor 1-alpha gene as a marker for diagnosing of Candida onychomycosis

Molecular Detection and Identification of Candida

Contact Info

Product

Resources

About