Draft genome sequence of Bacillus thuringiensis 147, a Brazilian strain with high insecticidal activity

Abstract

Bacillus thuringiensis is a ubiquitous Gram-positive and sporulating bacterium. Its crystals and secreted toxins are useful tools against larvae of diverse insect orders and, as a consequence, an alternative to recalcitrant chemical insecticides. We report here the draft genome sequence ofB. thuringiensis 147, a strain isolated from Brazil and with high insecticidal activity. The assembled genome contained 6,167,994 bp and was distributed in seven replicons (a chromosome and 6 plasmids). We identified 12 coding regions, located in two plasmids, which encode insecticidal proteins.

Role of plasmid plasticity and mobile genetic elements in the entomopathogen Bacillus thuringiensis serovar israelensis

“…The 147 and Br58 strains are the natural isolates (Barbosa et al. 2015 ; Zorzetti et al. 2015 ) containing all other B. thuringiensis sv.…”

Comparative genomic analysis and mosquito larvicidal activity of four Bacillus thuringiensis serovar israelensis strains

“…During sporulation, the gram-positive bacterium Bacillus thuringiensis (Bt) produces crystalline inclusions consisting of δ-endotoxins (Cry or Cyt proteins) with insecticidal activity 1 . Genomic analysis has contributed to the identification of new genes coding for toxins that are active against different insect species including orders such as Lepidoptera, Diptera [2][3][4][5][6][7] , and Coleoptera 8 . Proteins with nematicidal [9][10][11] and molluscicidal 12 activities have also been described.…”