Rickettsia amblyommii infecting Amblyomma sculptum in endemic spotted fever area from southeastern Brazil

Nunes, Emília de Carvalho; Vizzoni, Vinicius Figueiredo; Navarro, Daniel Leal; Iani, Felipe Campos de Melo; Durães, Liliane Silva; Daemon, Erik; Soares, Carlos A. G.

doi:10.1590/0074-02760150266

Cited by 18 publications

(14 citation statements)

References 33 publications

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“…3). In Brazil, R. amblyommatis has been detected in A. sculptum from the Pantanal (MT) (Alves et al, 2014), and in the Atlantic rainforest (Minas Gerais-MG) (Nunes et al, 2015). Ca.…”

Section: Discussionmentioning

confidence: 99%

“…Rickettsia amblyommatis has been suspected of being associated with an SF case in the United States (Apperson et al, 2008), and it was found to be slightly pathogenic for guinea pigs, especially when compared with R. rickettsii (Rivas et al, 2015). In Brazil, this rickettsia has already been serologically detected in dogs (Labruna et al, 2007;Melo et al, 2011), and horses (Alves et al, 2014), and in an area endemic for SF, it was molecularly identified in A. sculptum from MG (Nunes et al, 2015). Although all these data indicate that R. amblyommatis may be pathogenic for animals, including humans, its role in SF cases remains unclear.…”

Section: Discussionmentioning

confidence: 99%

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Amblyomma sculptum: genetic diversity and rickettsias in the Brazilian Cerrado biome

Bitencourth

Amorim

Oliveira

et al. 2017

Medical Vet Entomology

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Amblyomma sculptum (Ixodida: Ixodidae) Berlese, 1888 is the most important tick vector in Brazil, transmitting the bioagent of the most severe form of spotted fever (SF) in part of the Cerrado (in the states of Minas Gerais and São Paulo). In another part of the Cerrado (Central-West region of Brazil), a milder form of SF has been recorded. However, neither the rickettsia nor the vector involved have been characterized. The aim of the current study was to analyse genetic variation and the presence of rickettsia in A. sculptum in Cerrado, from silent areas and with the milder form of SF. Samples were subjected to DNA extraction, amplification and sequencing of 12S rDNA, cytochrome oxidase subunit II and D-loop mitochondrial genes (for tick population analyses), and gltA, htrA, ompA and gene D (sca4) genes for rickettsia researches. Exclusive haplotypes with low frequencies, high haplotype diversity and low nucleotide diversity, star-shaped networks and significant results in neutrality tests indicate A. sculptum population expansions in some areas. Rickettsia amblyommatis, Candidatus Rickettsia andeanae and Rickettsia felis were detected. The A. sculptum diversity is not geographically, or biome delimited, pointing to a different potential in vector capacity, possibly associated with differing tick genetic profiles.

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“…3). In Brazil, R. amblyommatis has been detected in A. sculptum from the Pantanal (MT) (Alves et al, 2014), and in the Atlantic rainforest (Minas Gerais-MG) (Nunes et al, 2015). Ca.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Amblyomma sculptum: genetic diversity and rickettsias in the Brazilian Cerrado biome

Bitencourth

Amorim

Oliveira

et al. 2017

Medical Vet Entomology

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“…Both A. sculptum and A. tonelliae are aggressive to humans and domestic mammals and they have been incriminated as potential vectors of pathogenic microorganisms for domestic animals and humans (Guedes et al, 2005;Widmer et al, 2011;Soares et al, 2012;Alves et al, 2014;Labruna et al, 2014;Nunes et al 2015;Tarragona et al, 2015a;Melo et al, 2016;Tarragona et al 2016). Fatal cases of spotted fever in humans due to the infection with Rickettsia rickettsii were reported in localities of Jujuy and Salta Provinces, Argentina, and ticks of the A. cajennense species complex were recognized as the possible vectors (Ripoll et al, 1999;Paddock et al, 2008;Seijo et al, 2016).…”

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confidence: 99%

Seasonal dynamics, geographical range size, hosts, genetic diversity and phylogeography of Amblyomma sculptum in Argentina

Tarragona

Sébastian

Bottero

et al. 2018

Ticks and Tick-borne Diseases

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The aim of this work was to generate knowledge on ecological aspects of Amblyomma sculptum in Argentina, such as seasonal dynamics, geographical range size, hosts, genetic diversity and phylogeography. Adult and immature A. sculptum ticks were collected in different localities of Argentina to know the geographical range size and hosts. The genetic diversity of this tick was studied through analyses of 16S rDNA sequences. To describe the seasonal dynamics, free-living ticks were monthly collected from October 2013 to October 2015. A. sculptum shows a marked ecological preference for Chaco Húmedo eco-region and "Albardones" forest of the great rivers in the wetlands in the Chaco Biogeographical Province, and for Selvas Pedemontanas and Selva Montana in the Yungas Biogeographical Province. This species has low host specificity, and it has large wild and domestic mammals as principal hosts to both immature and adult stages. Amblyomma sculptum is characterized by a one-year life cycle. Larvae peak in early winter, nymphs peaked during mid-spring, and adults during late summer and mid-summer. The genetic divergence was low and the total genetic variability was attributable to differences among populations. This fact could be associated to stochastics process linked to micro-habitat variations that could produce a partial restriction to gene flow among populations. The geographic regions do not contribute much to explain the A. sculptum population genetic structure, with an ancestral haplotype present in most populations, which gives rise to the rest of the haplotypes denoting a rapid population expansion.

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“…Candidatus R. andeanae foi primeiramente detectada em A. maculatum e I. boliviensis do Peru (BLAIR et al, 2004) (LABRUNA et al, 2004b, A. auricularium (SARAIVA et al, 2013) e A. sculptum (NUNES et al, 2015). Encontramos em nossos resultados apenas 1/200 (0,5%) A. sculptum infectado por esta riquétsia.…”

Section: Discussionunclassified

“…Em algumas espécies de carrapatos que ocorrem no Cerrado já foi demonstrada a infecção por riquétsias, como por exemplo Candidatus Rickettsia andeanae, um agente de patogenicidade desconhecida, infectando A. parvum (NIERI-BASTOS et al, 2014), Rickettsia amblyommii, outra bactéria de patogenicidade desconhecida, infectando A. sculptum (NUNES et al, 2015) e Rickettsia parkeri, um agente patogênico, infectando A. triste (SILVEIRA et al, 2015;MELO et al, 2015). Tais …”

Section: Introductionunclassified

<b>Ecologia de carrapatos e riquétsias transmitidas por carrapatos em uma reserva natural de cerrado brasileiro</i>

Barbieri¹

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A Deus pela vida e por todas as oportunidades que tem me dado.Aos meus pais, João Getulio (in memoriam) e Gislaine, pela criação, pelos valores morais que me ensinaram, pelo amor incondicional, por sempre acreditarem em mim e moldarem a pessoa que sou hoje.Ao meu filho, Lorenzo, que a cada dia faz de mim uma pessoa melhor.Aos meus irmãos, João Getulio (e família) e Vanessa, pelo amor e carinho.Ao meu orientador Marcelo, pelos direcionamentos, ensinamentos, pela sensibilidade, flexibilidade e liberdade que dá aos orientados. Agradeço à Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP)pelofinanciamento através da bolsa de doutorado para manutenção e reserva técnica para despesas com o trabalho (Processo nº 2012/10102-2). Agradeço à Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pelo auxílio financeiro e por tornar possível a realização deste trabalho.Ao meu "segundo orientador" Matias, pela paciência e carinho com que dedica seu tempo para ensinar com seriedade e descontração e por corrigir minha discussão.À professora Solange pela ajuda com a correção de parte deste trabalho escrito.Aos meus amigos, irmãos de alma, Herbert, Eveline e Monize, pelo companheirismo, amizade e força em todos os momentos. The Grande Sertão Veredas National Park (GSVNP) is one of the last natural reserves of Cerrado of Brazil, located in the northwest of Minas Gerais. This study involved 24 months of field work, which included eight campaigns at the park with intervals of three months, in order to determine the occurrence and ecological aspects of ticks and riquetsiaon the environment, small mammals and birds. In total 8488 ticks were collected during the campaigns. Through CO 2 traps 3050ticks were collected, being 2369 A. sculptum, 337 A. parvum, 233 A. triste, 13 A. dubitatum show the richness of ticks species, of small mammalsand birds species and also of Rickettsiaspecies found in the studied area inside the park.

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Rickettsia amblyommii infecting Amblyomma sculptum in endemic spotted fever area from southeastern Brazil

Cited by 18 publications

References 33 publications

Amblyomma sculptum: genetic diversity and rickettsias in the Brazilian Cerrado biome

Amblyomma sculptum: genetic diversity and rickettsias in the Brazilian Cerrado biome

Seasonal dynamics, geographical range size, hosts, genetic diversity and phylogeography of Amblyomma sculptum in Argentina

<b>Ecologia de carrapatos e riquétsias transmitidas por carrapatos em uma reserva natural de cerrado brasileiro</i>

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