Effects of penicillin and erythromycin on adherence of invasive and noninvasive isolates of Streptococcus pyogenes to laminin

Abstract

This study investigated the possible relationship between the invasiveness of group A Streptococcus (GAS) strains and their abilities to adhere to laminin and assessed the effects of subinhibitory concentrations of penicillin and erythromycin on the ability of GAS to adhere to laminin. The adherence of noninvasive and highly invasive isolates of GAS to laminin was significantly higher than the adherence displayed by isolates of low invasiveness. Antibiotic treatment caused significant reductions in adherence to laminin in all three groups of strains. Penicillin was more successful in reducing the adherence abilities of the tested GAS strains than erythromycin.

Cranberry and Sumac Extracts Exhibit Antibacterial and Anti-Adhesive Effects Against Streptococcus pyogenes

“…It has previously been shown that inhibitory concentrations of penicillin could stimulate the loss of lipoteichoic acid, consequently reducing the adherence of Streptococcus sanguinis to host tissue surfaces. 28 Significant reduction in the adhesion of GAS strains regardless of their degree of virulence (noninvasive, highly invasive, and low invasive) to laminin were observed after overnight exposure of the bacteria to 1/2 • MIC of penicillin G. 22 Comparable to our study, noninvasive, highly invasive, and low invasive isolates of S. pyogenes adhered more to the laminin-coated plates than to the uncoated plates. 22 GAS adhesion to uncoated and coated substratum was decreased to about £60%, with treatments of 1/2 • MBC to MBC concentrations of the sumac and cranberry extracts.…”

“…28 Significant reduction in the adhesion of GAS strains regardless of their degree of virulence (noninvasive, highly invasive, and low invasive) to laminin were observed after overnight exposure of the bacteria to 1/2 • MIC of penicillin G. 22 Comparable to our study, noninvasive, highly invasive, and low invasive isolates of S. pyogenes adhered more to the laminin-coated plates than to the uncoated plates. 22 GAS adhesion to uncoated and coated substratum was decreased to about £60%, with treatments of 1/2 • MBC to MBC concentrations of the sumac and cranberry extracts. To better evaluate the anti-adherence effects of fruit ANTI-STREPTOCOCCUS BERRY EXTRACTS extracts, we, in a cell study, examined the cranberry aqueous extract's attribute on the attachment of pretreated bacteria to the tonsil cells.…”

“…3,21 It is noteworthy that bacterial attachment to different substratum differs greatly among strains and is generally tissuedependent. 22 Some GAS strains are capable of binding to abiotic polystyrene surfaces, whereas others need a matrix or a protein-coated surface for successful attachment and adhesion. 22,23 For instance, isolates from asymptomatic GAS carriers are most likely to carry the fibronectin-binding protein F2 gene, and these isolates tend to have higher adherence capabilities than isolates from pharyngitis subjects.…”

“…22 Some GAS strains are capable of binding to abiotic polystyrene surfaces, whereas others need a matrix or a protein-coated surface for successful attachment and adhesion. 22,23 For instance, isolates from asymptomatic GAS carriers are most likely to carry the fibronectin-binding protein F2 gene, and these isolates tend to have higher adherence capabilities than isolates from pharyngitis subjects. 22 Our test strains showed similar adhesion capacity to both uncoated and BSA-coated substrates (Table 2 and Fig.…”

“…22,23 For instance, isolates from asymptomatic GAS carriers are most likely to carry the fibronectin-binding protein F2 gene, and these isolates tend to have higher adherence capabilities than isolates from pharyngitis subjects. 22 Our test strains showed similar adhesion capacity to both uncoated and BSA-coated substrates (Table 2 and Fig. 2).…”