Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections

Vasconcelos, Gentil Arthur Lins Bentes Mendonça de; Lanzarini, Natália Maria; Lima, Lyana Rodrigues Pinto; Manso, Pedro Paulo de Abreu; Silva, Alexandre dos Santos da; Mouta, Sergio da Silva e; Guimarães, Juliana Rodrigues; Moraes, Marcia Terezinha Baroni de; Pelajo-Machado, Marcelo; Pinto, Marcelo Alves

doi:10.1590/0074-02760140457

Cited by 12 publications

(4 citation statements)

References 20 publications

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“…More recently, IgY against HAV was used to detect the virus in hepatic sections of infected rhesus monkeys by means of indirect immunofluorescence (IIF). Anti-HVA IgY was more efficient than the commercially available IgG for the detection of the same antigen [105].…”

Section: Viral Infections Diagnosismentioning

confidence: 92%

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

Pereira

Tilburg

Florean

et al. 2019

International Immunopharmacology

105

118

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A B S T R A C TEgg yolk constitutes a relevant alternative source of antibodies. It presents some advantages over mammalian serum immunoglobulins regarding productivity, animal welfare and specificity. The main immunoglobulin present in avian blood (IgY) is transmitted to their offspring and accumulates in egg yolks, which enables the non-invasive harvesting of high amounts of antibodies. Moreover, due to structural differences and phylogenetic distance, IgY is more suitable for diagnostic purposes than mammalian antibodies, since it does not react with certain components of the human immune system and displays greater avidity for mammalian conserved proteins. IgY has been extensively used in health researches, as both therapeutic and diagnostic tool. This article aims to review its applications in both human and veterinary health.

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Section: Viral Infections Diagnosismentioning

confidence: 92%

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

Pereira

Tilburg

Florean

et al. 2019

International Immunopharmacology

105

118

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“…En la actualidad se exige una constante demanda de terapias que desarrollen inmunidad inmediata y a largo plazo, con una producción continua y escalable, que garantice pureza y suministro constante 90 . Los anticuerpos IgY se muestran como una inmunoterapia pasiva prometedora y eficaz en el tratamiento de muchas infecciones 91 , principalmente del sistema respiratorio, digestivo y oral 92 .…”

Section: Igy Como Terapia De Infecciones Humanasunclassified

Tecnología IgY: Estrategia en el tratamiento de enfermedades infecciosas humanas

Tipantiza

Nathaly

2021

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La aparición de microorganismos resistentes a antibióticos, el descubrimiento de nuevos agentes patógenos con potencial pandémico y el aumento de una población inmunocomprometida han dejado casi obsoleta la terapia antimicrobiana, terapia comúnmente usada para tratar enfermedades infecciosas. Por otro lado, las investigaciones acerca del uso del anticuerpo IgY para desarrollar inmunidad pasiva han demostrado el potencial que tiene la tecnología IgY para tratar enfermedades infecciosas víricas y bacterianas. Donde los anticuerpos IgY de aves se destacan por su alta especificidad, rendimiento y escalabilidad de producción a menor costo, con relación a los anticuerpos IgG de mamíferos. El objetivo de esta revisión es determinar la importancia del uso de los anticuerpos IgY como tratamiento terapéutico y profiláctico frente a los patógenos causantes de infecciones virales y bacterianas en humanos, mediante la recopilación de ensayos clínicos, productos comerciales y patentes registradas en el período de 2010-2021. Finalmente, con este estudio se estableció que la tecnología IgY es una herramienta biotecnológica versátil y eficaz para tratar y prevenir enfermedades infecciosas, al reducir los síntomas y la carga del patógeno.

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“…FITC-labeled anti-Mycobacterium avium subspecies paratuberculosis can target the pathogen inside the cytoplasm of infected macrophages [64]. Frozen liver fragments were diagnosed with cytomegalovirus antigens by using IgY-based confocal microscopy confirming the yolk antibody as suitable reagent for immunofluorescence assay [65].…”

Section: Immunofluorescence Assaymentioning

confidence: 99%

IgY-Technology Applied to Studies of Toxoplasma gondii Infection

Júnior¹,

Santos²,

Bassi³

et al. 2017

Toxoplasmosis

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In this chapter, we describe relevant aspects of immunoglobulin Y (IgY) technology for Toxoplasma gondii applications, including comparison of avian IgY antibody with mammalian IgG antibody, egg yolk IgY production and isolation procedures, important applications for IgY antibody, and state of the art and perspectives for IgY-technology in T. gondii studies. T. gondii is a worldwide public health problem. IgY-technology provides an alternative antibody (IgY) to mammalian Immunoglobulin G (IgG) antibody. IgY-technology involves the chicken immunization, yolk IgY isolation, antibody characterization, and purified IgY application to several kinds of methods. Immunized chicken transfers a specific IgY from blood to egg yolk. Phylogenetic distance between chickens and mammals influences the generation of antibody repertoires recognizing an antigen profile. IgY is not bound to rheumatoid factor or mammalian complement protein and thus avoids the false-positive results. Yolk IgY isolation is carried out by simple procedures that are accessible for any laboratory and, also, for IgY isolation at large-scale production. IgY-technology provides antibodies for proteomic studies, diagnostic assays, and immunotherapy. Although IgY-technology is promising, there is a reduced number of investigations with IgY and T. gondii. Future perspectives involve the use of IgY-technology for the screening of new T. gondii antigens for diagnostics, therapy, or vaccine, development of innovative techniques for toxoplasmosis diagnostics and may be an immunotherapy for toxoplasmosis.

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Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections

Cited by 12 publications

References 20 publications

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

Tecnología IgY: Estrategia en el tratamiento de enfermedades infecciosas humanas

IgY-Technology Applied to Studies of Toxoplasma gondii Infection

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