Full-genome sequences of hepatitis B virus subgenotype D3 isolates from the Brazilian Amazon Region

Spitz, Natália; Mello, Francisco Campello do Amaral; Araújo, Natalia M.

doi:10.1590/0074-02760140426

Cited by 7 publications

(7 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, all Brazilian D3 clustered together, suggesting closer ancestral relationships (intragroup divergence of 0.005 ± 0.001, versus 0.007 ± 0.001 when compared to international sequences). However, a study enrolling full-genome analysis of HBV/D3 from Brazilian Amazon Region published by Spitz and colleagues [43] did not find a close relatedness between the Brazilian and European D3 sequences. However, this might be due to the limited number of HBV/D3 full-length genomes assigned to Italy in Genbank that hindered more robust phylogenetic analyses.…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of the Hepatitis B Virus Subgenotypes in Brazil

Lago

Espírito-Santo

Costa

et al. 2019

Viruses

View full text Add to dashboard Cite

Hepatitis B virus (HBV) subgenotypes may be related to clinical outcomes and response to antiviral therapy. Most Brazilian studies on HBV subgenotypes are restricted to some regions and to specific population groups. Here, we provide an insight about genetic diversity of HBV subgenotypes in 321 serum samples from all five geographical regions, providing a representative overview of their circulation among chronic carriers. Overall, HBV/A1 was the most prevalent subgenotype, being found as the major one in all regions except in South Brazil. Among HBV/D samples, subgenotype D3 was the most prevalent, found in 51.5%, followed by D2 (27.3%) and D4 (21.2%). D2 and D3 were the most prevalent subgenotypes in South region, with high similarity with European strains. D4 was found in North and Northeast region and clustered with strains from Cape Verde and India. For HBV/F, the most frequent subgenotype was F2 (84.1%), followed by F4 (10.1%) and F1 (5.8%), closely related with strains from Venezuela, Argentina and Chile, respectively. Phylogeographic analyses were performed using an HBV full-length genome obtained from samples infected with genotypes rarely found in Brazil (B, C, and E). According to Bayesian inference, HBV/B2 and HBV/C2 were probably introduced in Brazil through China, and HBV/E from Guinea, all of them mostly linked to recent events of human migration. In conclusion, this study provided a comprehensive overview of the current circulation of HBV subgenotypes in Brazil. Our findings might contribute to a better understand of the dynamics of viral variants, to establish a permanent molecular surveillance on the introduction and dispersion patterns of new strains and, thus, to support public policies to control HBV dissemination in Brazil.

show abstract

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of the Hepatitis B Virus Subgenotypes in Brazil

Lago

Espírito-Santo

Costa

et al. 2019

Viruses

View full text Add to dashboard Cite

show abstract

“…Accordingly, previous reports on the times of origin and divergence of HBV/D and its subgenotypes have suggested different estimates [23, 24, 26–29]. In the Americas, HBV/D is found across the continent [16, 30–35], although the detailed evolutionary history and phylogeography of this genotype have not yet been examined. In addition, few HBV/D complete genome sequences from Brazil are available in the databanks, limiting the contribution of Brazilian isolates to phylogenetic and phylogeographic studies.…”

Section: Introductionmentioning

confidence: 99%

Reconstruction of the spatial and temporal dynamics of hepatitis B virus genotype D in the Americas

et al. 2019

Self Cite

View full text Add to dashboard Cite

Hepatitis B virus (HBV) genotype D (HBV/D) is globally widespread, and ten subgenotypes (D1 to D10) showing distinct geographic distributions have been described to date. The evolutionary history of HBV/D and its subgenotypes, for which few complete genome sequences are available, in the Americas is not well understood. The main objective of the current study was to determine the full-length genomic sequences of HBV/D isolates from Brazil and frequency, origin and spread of HBV/D subgenotypes in the Americas. Complete HBV/D genomes isolated from 39 Brazilian patients infected with subgenotypes D1 (n = 1), D2 (n = 10), D3 (n = 27), and D4 (n = 1) were sequenced and analyzed together with reference sequences using the Bayesian coalescent and phylogeographic framework. A search for HBV/D sequences available in GenBank revealed 209 complete and 926 partial genomes from American countries (Argentina, Brazil, Canada, Chile, Colombia, Cuba, Haiti, Martinique, Mexico, USA and Venezuela), with the major circulating subgenotypes identified as D1 (26%), D2 (17%), D3 (36%), D4 (21%), and D7 (1%) within the continent. The detailed evolutionary history of HBV/D in the Americas was investigated by using different evolutionary time scales. Spatiotemporal reconstruction analyses using short-term substitution rates suggested times of the most recent common ancestor for the American HBV/D subgenotypes coincident with mass migratory movements to Americas during the 19 th and 20 th centuries. In particular, significant linkages between Argentina and Syria (D1), Brazil and Central/Eastern Europe (D2), USA and India (D2), and Brazil and Southern Europe (D3) were estimated, consistent with historical and epidemiological data.

show abstract

“…6,7 Therefore, it is not surprising that several research efforts in the last decade have been directed to discover the different HBV sub-genotyping in several countries. [8][9][10][11] During HBV replication, the lack of proofreading ability of the reverse transcriptase leads to an error rate that is 10-fold higher than Mohammed El-Mowafy and Abdelaziz Elgaml contributed equally in this work.…”

mentioning

confidence: 99%

Molecular analysis of Hepatitis B virus sub‐genotypes and incidence of preS1/preS2 region mutations in HBV‐infected Egyptian patients from Mansoura

El‐Mowafy

Elgaml

El-Mesery

et al. 2017

Journal of Medical Virology

View full text Add to dashboard Cite

Hepatitis B virus (HBV) is one of the major causes of viral hepatitis worldwide. Despite the prevalence of HBV infection in Egypt, few studies have focused on sub-genotyping of the virus. Moreover, no studies are available regarding the mutational analysis of the preS1/preS2 region of the viral genome, or its impact on hepatocellular carcinoma (HCC) development in Egypt. In this study, we have analyzed the sub-genotypes and incidence of mutations in the preS1/preS2 region of HBV present in HBV-infected patients, from Mansoura city (located in the center of Nile Delta region of Egypt), via partial sequencing of this specific region. Moreover, we have investigated the impact of these mutations on HCC development by measuring serum alpha fetoprotein (AFP) level and abdominal ultrasound examination of the HBV-infected patients. According to our results, all samples were genotype D in which sub-genotype D1 was predominant. In addition, the results revealed mutations in the preS1/preS2 region, which could result in either immature preS1 protein or completely inhibit the translation of the preS2 protein. However, there was no incidence of HCC development in patients infected with mutated HBV in the preS1/preS2 region. In summary, for the first time our work has proved the predominance of sub-genotype D1 among HBV-infected Egyptian patients in Mansoura city, Nile Delta region, Egypt, and incidence of mutations in the preS1/preS2 region of HBV genome. This current study opens up research opportunities to discuss the impact of HBV mutations on the development of HCC in Egypt.

show abstract

Full-genome sequences of hepatitis B virus subgenotype D3 isolates from the Brazilian Amazon Region

Cited by 7 publications

References 13 publications

Genetic Diversity of the Hepatitis B Virus Subgenotypes in Brazil

Genetic Diversity of the Hepatitis B Virus Subgenotypes in Brazil

Reconstruction of the spatial and temporal dynamics of hepatitis B virus genotype D in the Americas

Molecular analysis of Hepatitis B virus sub‐genotypes and incidence of preS1/preS2 region mutations in HBV‐infected Egyptian patients from Mansoura

Contact Info

Product

Resources

About