Hemolytic and urease activities in vibrios isolated from fresh and frozen oysters

Costa, Renata Albuquerque; Araújo, Rayza Lima; Vieira, Regine Helena Silva dos Fernandes

doi:10.1590/0037-868210722013

Cited by 4 publications

(4 citation statements)

References 13 publications

(12 reference statements)

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“…H. hibernica has the ability to grow at 4 °C; processing food at lower temperatures is a beneficial trait as it hinders the propagation of many spoilage and pathogenic organisms, which are more often mesophilic [90]. H. hibernica was negative for haemolysis and pathogenicity islands, which are potential virulence factors that would not be desirable in a food processing industry [91][92][93][94].…”

Section: Discussionmentioning

confidence: 99%

Genome analysis and phenotypic characterization of Halomonas hibernica isolated from a traditional food process with novel quorum quenching and catalase activities

2022

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Traditional food processes can utilize bacteria to promote positive organoleptic qualities and increase shelf life. Wiltshire curing has a vital bacterial component that has not been fully investigated from a microbial perspective. During the investigation of a Wiltshire brine, a culturable novel bacterium of the genus Halomonas was identified by 16S rRNA gene (MN822133) sequencing and analysis. The isolate was confirmed as representing a novel species (Halomonas hibernica B1.N12) using a housekeeping (HK) gene phylogenetic tree reconstruction with the selected genes 16S rRNA, 23S rRNA, atpA, gyrB, rpoD and secA. The genome of the new isolate was sequenced and annotated and comparative genome analysis was conducted. Functional analysis revealed that the isolate has a unique phenotypic signature including high salt tolerance, a wide temperature growth range and substrate metabolism. Phenotypic and biochemical profiling demonstrated that H. hibernica B1.N12 possesses strong catalase activity which is an important feature for an industrial food processing bacterium, as it can promote an increased product shelf life and improve organoleptic qualities. Moreover, H. hibernica exhibits biocontrol properties based on its quorum quenching capabilities. Our work on this novel isolate advances knowledge on potential mechanistic interplays operating in complex microbial communities that mediate traditional food processes.

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Section: Discussionmentioning

confidence: 99%

Genome analysis and phenotypic characterization of Halomonas hibernica isolated from a traditional food process with novel quorum quenching and catalase activities

2022

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“…Biofilms have also been thought to play a crucial part (Silva & Benitez 2016). Reports on haemolysis in Vibrio sp., in Nigeria are few, but reports from around the globe show that haemolytic activity within our strains was relatively low with studies reporting rates ranging from 20.5% to 36% (Costa et al, 2013;Arunagiri & Sivakumar 2021). The much higher rates of biofilm-forming ability of isolates in this study were similar to reports by a previous study which noted biofilm-forming potential in 56.14% of their test isolates (Santajit et al, 2022).…”

Section: Discussionmentioning

confidence: 57%

Potentially pathogenic multidrug-resistant <i>Vibrio</i> sp. isolated from dumpsites in Rivers State, Nigeria

Otokunefor,

Nwokolo,

Abara

et al. 2024

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Vibrio sp. is associated with several disease conditions. An assessment of the pathogenic potential of such strains is key in assessing the risk these organisms could pose to human health. This study therefore was aimed at analyzing the pathogenic potential of MDR Vibrio sp. isolated from select dumpsites in Port Harcourt, Rivers State. Two dumpsites in Port Harcourt, Rivers State were assessed for the presence of Vibrio sp. using standard microbiological techniques involving a pre-enrichment using alkaline peptone water, culture on Thiosulfate-citrate-bile salts-sucrose and biochemical characterisation. Following antimicrobial susceptibility testing using the standard Kirby Bauer disc diffusion method, pathogenic potential was assessed based on haemolytic activity and biofilm forming potential. Results showed that the highest rates of resistance were against cefuroxime (68.8%) while the lowest resistance rates (3.2%) were against the quinolone antibiotics (ofloxacin and ciprofloxacin). In total, 38 antibiograms were observed among the 93 isolates with AUG-CAZ-CRX-CXM noted as the most commonly occurring antibiogram. Only 31.2% (29/93) of isolates were multidrug resistant. A total of 47.3% of the test isolates exhibited pathogenic potential (either haemolytic ability or biofilm forming potential), with more (37.6%) exhibiting biofilm forming potential. A co-occurrence of both pathogenic characteristics was observed in only 6 (13.6%) isolates. Of the 93 isolates, a co-occurrence of MDR and pathogenic potential was observed in 16.1% of isolates (15/93). This study shows a moderate association between potentially pathogenic multidrug resistance Vibrio sp. and the sampled dumpsites indicating a low potential public health risk because of the non-pathogenic nature of strains isolated.

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“…Costa et al . () found 11·4% V. vulnificus presumptive in samples from frozen oysters, which were confirmed through biochemical tests. However, in other studies, the prevalence is low as 4% (Pereira et al .…”

Section: Vibrio Vulnificus Occurrence In South Americamentioning

confidence: 69%

“…,b; Ramos , ; Costa et al . ); there are only three infection cases reported, and two of them occurred in 2004 in areas that are close to one another. The first case reported in Brazil was related to an injury caused by a wood fragment, in a 53‐year‐old man, in Rio de Janeiro state.…”

Section: Vibrio Vulnificus Occurrence In South Americamentioning

confidence: 99%

Vibrio parahaemolyticusandVibrio vulnificusin South America: water, seafood and human infections

et al. 2016

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The bacterial species, Vibrio parahaemolyticus and Vibrio vulnificus, are ubiquitous in estuaries and coastal waters throughout the world, but they also happen to be important human pathogens. They are concentrated by filter-feeding shellfish which are often consumed raw or undercooked, providing an important potential route of entry for an infective dose of these bacteria. Vibrio parahaemolyticus can cause abdominal cramping, nausea, diarrhoea, vomiting, chills and fever. Vibrio vulnificus can cause similar gastrointestinal-related symptoms, but can also spread to the bloodstream, resulting in primary septicaemia, and it can also cause disease via wound infections. The objective of this article is to summarize, for the first time, the incidence and importance of V. parahaemolyticus and V. vulnificus in South America, in environmental waters and seafood, especifically molluscan shellfish, as well as human infection cases and outbreaks. It appears that infections from V. parahaemolyticus have been more strongly related to shellfish ingestion and have been more frequently reported on the Pacific coast of South America. Conversely, V. vulnificus has been more frequently acquired by water contact with open wounds and its presence has been more heavily reported along the Atlantic coast of South America, and while documented to cause serious mortality, have been relatively few in number. The impacts of El Nino Southern Oscillation (ENSO) have been observed to cause an increase in V. parahaemolyticus outbreaks on the Pacific coast of South America. The implementation of a regulated monitoring approach, along with the use of faster, more accurate and virulence-specific detection approaches, such as PCR confirmation, should be considered to detect the presence of pathogenic Vibrio strains in environmental and seafood samples for protection of public health. Furthermore, improved clinical surveillance with suspected cases should be implemented. This review highlights the need for more research and monitoring of vibrios in South America, in water, shellfish and clinical samples.

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Hemolytic and urease activities in vibrios isolated from fresh and frozen oysters

Cited by 4 publications

References 13 publications

Genome analysis and phenotypic characterization of Halomonas hibernica isolated from a traditional food process with novel quorum quenching and catalase activities

Genome analysis and phenotypic characterization of Halomonas hibernica isolated from a traditional food process with novel quorum quenching and catalase activities

Potentially pathogenic multidrug-resistant <i>Vibrio</i> sp. isolated from dumpsites in Rivers State, Nigeria

Vibrio parahaemolyticusandVibrio vulnificusin South America: water, seafood and human infections

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