Evaluation of CD4+CD25+ T lymphocyte response time kinetics in patients with chronic Chagas disease after in vitro stimulation with recombinant Trypanosoma cruzi antigens

Braz, Suellen Carvalho de Moura; Lorena, Virgínia Maria Barros de; Melo, Adriene Siqueira de; Cavalcanti, Maria da Gloria Aureliano Melo; Gomes, Yara de Miranda

doi:10.1590/0037-8682-1323-2013

Cited by 2 publications

(1 citation statement)

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“…In the same disease, specific CD4 + T-cell patients by OX40/CD40L [26], OX40/CD69 [25] or CD69/ CD137 [24] AIM combinations after 24 h of stimulation. The individual expression of some activation markers, that is, CD25, HLA-DR and CD69, were mainly explored to interrogate the ability of certain T. cruzi proteins to elicit cellular response in patients with CCD [7,44,45]. However, to our knowledge, we are the first using any combination of surface markers in AIM assay to measure in vitro agnostic CD4 + T-cell activation in the context of this infection [13].…”

Section: Discussionmentioning

confidence: 99%

Activation‐induced marker assays for identification of Trypanosoma cruzi‐specific CD4 or CD8 T cells in chronic Chagas disease patients

et al. 2023

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Antigen-specific T cells are central to the adaptive immune response against T. cruzi infection and underpin the efficacy of on-going vaccine strategies. In this context, the present study focuses on T-cell assays that define the parasitespecificity on the basis of upregulation of TCR stimulation-induced surface markers. For this purpose, we tested different dual marker combinations (OX40, CD25, CD40L, CD137, CD69, PD-L1, CD11a, CD49d, HLA-DR, CD38) to reliably identify activated CD4 + and CD8 + T-cell populations from PBMCs of chronic Chagas disease (CCD) patients after 12 or 24 h of stimulation with T. cruzi lysate.Results demonstrated that activation-induced markers (AIM) assays combining the expression of OX40, CD25, CD40L, CD137, CD69 and/or PD-L1 surface markers are efficient at detecting T. cruzi-specific CD4 + T cells in CCD patients, in comparison to non-infected donors, after both stimulation times. For CD8 + T cells, only PD-L1/OX40 after 24 h of antigen exposure resulted to be useful to track a parasite-specific response. We also demonstrated that the agnostic activation is mediated by different T. cruzi strains, such as Dm28c, CL Brener or Sylvio.Additionally, we successfully used this approach to identify the phenotype of activated T lymphocytes based on the expression of CD45RA and CCR7. Overall, our results show that different combinations of AIM markers represent an effective and simple tool for the detection of T. cruzi-specific CD4 + and CD8 + T cells.

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