Detection of multidrug-resistant Pseudomonas aeruginosa harboring bla GES-1 and bla GES-11 in Recife, Brazil

Abstract

Introduction:Pseudomonas aeruginosa, an important pathogen globally, presents several resistance mechanisms. This study aimed to investigate the presence of bla GES in clinical isolates of Pseudomonas aeruginosa obtained from various clinical specimens from patients admitted to three different hospitals in Recife, Brazil. The Guiana extended spectrum beta-lactamase (GES) enzymes are responsible for conferring broad spectrum resistance to beta-lactam drugs, including the carbapenems. Methods: A total of 100 carbapenem-resistant P. aeruginosa isolates underwent polymerase chain reaction (PCR) testing to identify bla GES, bla KPC, bla SPM-1, bla IMP , and bla VIM . Additionally, PCR products positive for bla GES were sequenced. The clonal profi les of these same isolates were then determined by means of enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis. Results: PCR analysis revealed that four isolates harbored bla GES ; DNA sequencing showed that two harbored bla GES-1 and two bla . Beta-lactamase genes bla SPM-1 , bla IMP , bla VIM , and bla KPC were investigated; none of these genes was detected. Automated susceptibility testing methods (Vitek®2, bioMérieux) showed that the bla GES-1 -positive isolates were only susceptible to polymyxin B. The patterns obtained with ERIC-PCR methods showed clonal relationship between the two isolates that harbored bla , whereas different clonal profi les were found in the isolates harboring bla GES-1. Conclusions: We detected the presence of bacterial isolates positive for two different variants of the enzyme GES in three different hospitals from Recife, Brazil. These enzymes have a great capacity for dissemination among Gram-negative bacteria and confer broad-spectrum resistance to betalactam antibiotics and to the carbapenems.

Synergistic Effect between Usnic Acid and Polymyxin B against Resistant Clinical Isolates of Pseudomonas aeruginosa

“…Currently, only a few antimicrobial agents are under development; what causes concern the scientific community is the emergence of resistance mechanisms that are being constantly reported around the world. Thus, research on new therapies is of extreme importance especially regarding resistant strains of P. aeruginosa , for this microorganism is the most commonly involved pathogen in infected burns and wounds, as well as in systemic infections of hospitalized patients [ 3 , 13 ].…”

“…Studies developed in Brazil between 2009 and 2017 reported that drugs such as the polymyxins should be the last therapeutic resource against multidrug-resistant strains of P. aeruginosa and Acinetobacter spp. Besides, pan-resistant strains of these same species have been found in clinical isolates from patients admitted in intensive care units (ICUs) in Brazilian hospitals [3,28,29].…”

“…us, research on new therapies is of extreme importance especially regarding resistant strains of P. aeruginosa, for this microorganism is the most commonly involved pathogen in infected burns and wounds, as well as in systemic infections of hospitalized patients [3,13]. e perspectives of this study include performing in vivo experiments to evaluate the combination between usnic acid and polymyxin B for the treatment of wound infections, and other superficial or systemic infections caused by polymyxin B-resistant P. aeruginosa, as well as the encapsulation of usnic acid into liposomes and the evaluation of the in vivo antibacterial activity of this Lipo-usnic acid with polymyxin B.…”

“…e wide variety of virulence and resistance mechanisms presented by P. aeruginosa and Acinetobacter spp. isolates, as well as their capacity of interacting and exchanging genetic material with other bacteria, stimulates the search for resistance genes in isolates found in hospital environments [2][3][4]. Besides, the increasing number of infections caused by multidrug-resistant (MDR) Gram-negative strains led to the return of the polymyxins (polymyxin B and colistin) in medical clinics as one of the treatment options [5,6].…”

Análise Espacial Da Paisagem E a Incidência Da Cochonilha-Do-Carmim (Dactylopius Opuntiae) Em Palma Forrageira No Estado De Alagoas

“…(RIBEIRO et al, 2013;TUON et al, 2015). Um fator determinante nas infecções nosocomiais por Enterobacter, está na capacidade de resistência a mais de um grupo de antibióticos como a produção de metalo-β-lactamases (MβL), β-lactamases de espectro estendido (ESBL) e carbapenemases do tipo KPC, sendo crescente o número de relatos de surgimento de novas bactérias com características de resistência a esta classe de antibióticos (Figura 2) (WHO, 2014;JÁCOME et al, 2016;SILVA JÚNIOR et al, 2017)Acinetobacter spp. and Klebsiella spp.…”