Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss mice

Carvalho, Letícia Helena de; Teixeira, Leda Fabiélen; Zaqueo, Kayena Delaix; Bastos, Jéssica Felix; Néry, Neriane Monteiro; Setúbal, Sulamita S.; Pontes, Adriana Silva; Butzke, Diana S.; Cavalcante, Walter Luís Garrido; Gallacci, Márcia; Fernandes, Carla Freire Celedônio; Stábeli, Rodrigo G.; Soares, Andreimar M.; Zuliani, Juliana Pavan

doi:10.1590/0037-8682-0526-2018

Cited by 11 publications

(9 citation statements)

References 54 publications

(66 reference statements)

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“…The clear supernatant was then applied to a Sephadex G-75 column equilibrated with the same buffer; fractions of 0.1 mL/tube were collected and monitored at Abs 280 nm using an automatic fraction collector. Purification of crotoxin and subunits [crotoxin A (crotapotin) and crotoxin B (CB or Asp 49-PLA2)] was performed as described elsewhere with minimal changes [ 30 , 31 , 32 , 33 , 34 , 35 ]. The elution was monitored at an absorbance of 280 nm, manually collected, lyophilized, and stored at -20 °C.…”

Section: Methodsmentioning

confidence: 99%

Cytotoxic effects of crotoxin from Crotalus durissus terrificus snake in canine mammary tumor cell lines

Pedro,

Brasileiro,

Macedo

et al. 2024

J. Venom. Anim. Toxins incl. Trop. Dis

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Background: Mammary gland tumors are the most prevalent neoplasm in intact female dogs, and they are good natural models to study comparative oncology. Most canine mammary malignancies, as in women, are commonly refractory to conventional therapies and demand continuous new therapeutic approaches. Crotalus durissus terrificus , also called rattlesnake, has more than 60 different proteins in its venom with multiple pharmaceutical uses, such as antitumor, antiviral, and antimicrobial action. Crotoxin, a potent β-neurotoxin formed by the junction of two subunits, a basic subunit (CB-PLA 2 ) and an acidic subunit (crotapotin), has already been reported to have anticancer properties in different types of cancers. Methods: In this work, we describe the cytotoxic potential of crotoxin and its subunits compared to doxorubicin (drug of choice) in two canine mammary carcinoma cell lines. Results: Crotoxin, CB-PLA 2 , crotalic venom, and doxorubicin decreased cell viability and the ability to migrate in a dose-dependent manner, and crotapotin did not present an antitumoral effect. For all compounds, the predominant cell death mechanism was apoptosis. In addition, crotoxin did not show toxicity in normal canine mammary gland cells. Conclusion: Therefore, this work showed that crotoxin and CB-PLA 2 had cytotoxic activity, migration inhibition, and pro-apoptotic potential in canine mammary gland carcinoma cell lines, making their possible use in cancer research.

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Section: Methodsmentioning

confidence: 99%

Cytotoxic effects of crotoxin from Crotalus durissus terrificus snake in canine mammary tumor cell lines

Pedro,

Brasileiro,

Macedo

et al. 2024

J. Venom. Anim. Toxins incl. Trop. Dis

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“…Local manifestations are commonly associated with the inflammatory properties that venom can trigger once inoculated. Although toxins from C. durissus subspecies are known to present low immunogenicity, studies have shown the capacity of venoms and toxins to promote local and systemic inflammatory responses in animal models ( 78 , 121 , 122 ). As previously described, C.d.…”

Section: Human Envenomations Caused By C D Ruruimamentioning

confidence: 99%

Crotalus Durissus Ruruima: Current Knowledge on Natural History, Medical Importance, and Clinical Toxinology

Pucca

Bernarde

Rocha³

et al. 2021

Front. Immunol.

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Crotalus durissus ruruima is a rattlesnake subspecies mainly found in Roraima, the northernmost state of Brazil. Envenomings caused by this subspecies lead to severe clinical manifestations (e.g. respiratory muscle paralysis, rhabdomyolysis, and acute renal failure) that can lead to the victim’s death. In this review, we comprehensively describe C. d. ruruima biology and the challenges this subspecies poses for human health, including morphology, distribution, epidemiology, venom cocktail, clinical envenoming, and the current and future specific treatment of envenomings by this snake. Moreover, this review presents maps of the distribution of the snake subspecies and evidence that this species is responsible for some of the most severe envenomings in the country and causes the highest lethality rates. Finally, we also discuss the efficacy of the Brazilian horse-derived antivenoms to treat C. d. ruruima envenomings in Roraima state.

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“…Aquí se observó un incremento en la liberación de CKT, CK-MB y LDH, lo cual indicó miotoxicidad y ALT, AST, GGT y ALP. Esto produjo daño hepatotóxico y provocó un bloqueo neuromuscular (46). Esta revista incorpora la opción Online First, mediante la cual las versiones definitivas de los trabajos aceptados son publicadas en línea antes de iniciar el proceso de diseño de la revista impresa.…”

Section: Fuente: Elaboración Propiaunclassified

Efectos bioquímicos e histopatológicos en ratas inducidos por el veneno de Crotalus durissus cumanensis del municipio Falcón, estado Falcón, Venezuela

Quintero

Graterol

2020

Rev. Med. Vet.

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El accidente ofídico representa un importante problema de salud pública en las zonas tropicales y subtropicales del mundo. La cascabel común (Crotalus durissus cumanensis) es la serpiente del género Crotalus más abundante en Venezuela. El objetivo fue determinar los cambios séricos en la alanino aminotransferasa (ALT), aspartato aminotransferasa (AST), lactato deshidrogenasa (LDH) y creatina fosfocinasa (CK) en ratas, inducidos por el veneno de Crotalus durissus cumanensis, así como el establecimiento de los efectos histopatológicos. Se prepararon cinco grupos experimentales con tres ratas cada uno, reservando un grupo control placebo (G1), el cual fue tratado por ruta IP con 100 μL de solución salina fisiológica estéril (SSFe). Al resto de los animales se les administraron 50 μg de veneno nativo por la misma vía. Estos fueron sacrificados a las 1 (G2), 3 (G3), 6 (G4) y 9 (G5) horas posinoculación, con tomas de muestras séricas para determinaciones de las referidas enzimas y de diferentes órganos para estudios histopatológicos (corazón, músculo esquelético, hígado y riñón). Se observó un incremento en la ALT (p 0,005) a partir de la primera hora posinyección, con un pico a la novena hora de 147 ± 8 UI/L; para la AST (p 0,005) a partir de la primera hora posinyección con concentración pico a la sexta hora (293 ± 8 UI/L). De la LDH (p 0,005) se registró un pico máximo de 2700 ± 8 UI/L a la novena hora posinyección. La CK (p 0,005) mostró un pico máximo de concentración a la sexta hora (1489,66 ± 8,5 UI/L). Los resultados histopatológicos en tejido cardíaco mostraron hiperemia, congestión y necrosis de Zenker; en músculo esquelético, hiperemia, infiltrado inflamatorio, necrosis de Zenker; en hígado, extravasación de glóbulos rojos, congestión de sinusoides, telangiectasia e infiltrado inflamatorio en vena centrolobulillar. El riñón mostró hemorragia en túbulos contorneados, infiltrado inflamatorio, colapso de los túbulos hasta la pérdida de la arquitectura del órgano. Los efectos observados fueron tiempo-dependientes. Los resultados pueden indicar un posible efecto miotóxico y hepatotóxico

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Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss mice

Cited by 11 publications

References 54 publications

Cytotoxic effects of crotoxin from Crotalus durissus terrificus snake in canine mammary tumor cell lines

Cytotoxic effects of crotoxin from Crotalus durissus terrificus snake in canine mammary tumor cell lines

Crotalus Durissus Ruruima: Current Knowledge on Natural History, Medical Importance, and Clinical Toxinology

Efectos bioquímicos e histopatológicos en ratas inducidos por el veneno de Crotalus durissus cumanensis del municipio Falcón, estado Falcón, Venezuela

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