First case report of cryptococcosis due to Cryptococcus decagattii in a pediatric patient in Argentina

Berejnoi, Ana; Taverna, Constanza Giselle; Mazza, Mariana; Vivot, Matías; Isla, Guillermina; Córdoba, Susana; Davel, Graciela

doi:10.1590/0037-8682-0419-2018

Cited by 11 publications

(8 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It was first named genotype VGIIIc 3,49 but recently renamed VGVI 50,51 . Recently, a case report of cryptococcosis caused by C. decagattii in Argentina was published in a patient belonging to a community living in huts made of branches in close physical contact with nature and domestic animals 48 . Our results are surprising because cases of cryptococcosis due to C. tetragattii are rare and even more common in Europe, and we described a new ST 556.…”

Section: Discussionmentioning

confidence: 69%

See 1 more Smart Citation

Multilocus sequence typing of strains from the Cryptococcus gattii species complex from different continents

Bellet

Roger

Krasteva

et al. 2021

Mycoses

View full text Add to dashboard Cite

Background Cryptococcus neoformans and Cryptococcus gattii species complexes are pathogens causing cryptococcal meningitis, a fungal infection that leads to death unless treated. Worldwide, it is estimated to kill over 180,000 individuals annually. Objectives We aim to investigate the molecular diversity of C. gattii isolates from strains isolated from 1995 to the present day from different continents. Method In this study, we analysed the molecular diversity by MLST and antifungal susceptibility by using the broth microdilution method according to the CLSI M27‐A4 protocol of a total of 26 strains from Cryptococcus gattii species complex from both clinical and environmental sources. Results Genotyping showed that most of the strains (17/26; 65.4%) belonged to serotype B and were distributed between three genotypes: VGI (13/17; 76.5%), VGII (3/17; 17.6%) and VGVI (1/17; 5.9%). The serotype C strains (9/26; 34.6%) were distributed between the VGIII (1/9; 11.1%) and VGIV (8/9; 88.9%) genotypes. The 26 strains belonged to 17 different MLST subtypes, and we highlight four new MLST genotypes (ST553, 554, 555 and 556). The two environmental strains were identified as serotype B and genotype VGI, but were of ST 51 and 154. All isolates have wild‐type MIC of fluconazole and flucytosine. Regarding amphotericin B, five VGI strains showed MICs to AMB equal to 1 µg/ml, and according to the ECV for these genotypes, they were considered non‐wild‐type strains. Conclusions The current study reveals the genetic diversity and new sequence types among strains from the C. gattii complex species.

show abstract

Section: Discussionmentioning

confidence: 69%

“…Among the VGVI strains, we identified one C. decagattii strain, serotype B, which came from Spain. C. decagatti has rarely been isolated, and there are few reports worldwide 3,48 . It was first named genotype VGIIIc 3,49 but recently renamed VGVI 50,51 .…”

Section: Discussionmentioning

confidence: 99%

Multilocus sequence typing of strains from the Cryptococcus gattii species complex from different continents

Bellet

Roger

Krasteva

et al. 2021

Mycoses

View full text Add to dashboard Cite

show abstract

“…In the literature, less than a thousand cases of cryptococcal infection have been reported in children worldwide; in Latin America, no more than 50 cases have been described [32][33][34]. Caused by both C. neoformans and C. gattii species complexes, cryptococcosis cases in otherwise healthy and HIV-infected children has been documented in Argentina [35], Bolivia [35], Brazil [36,37] and Colombia [33,38]. In the case reported here, a VNII isolate (ST43) was recovered from the 9-year-old Peruvian girl.…”

Section: Discussionmentioning

confidence: 99%

Cryptococcus neoformans Causing Meningoencephalitis in Adults and a Child from Lima, Peru: Genotypic Diversity and Antifungal Susceptibility

Firacative

Zuluaga-Puerto

Guevara

2022

JoF

View full text Add to dashboard Cite

Cryptococcosis, caused predominantly by Cryptococcus neoformans, is a potentially fatal, opportunistic infection that commonly affects the central nervous system of immunocompromised patients. Globally, this mycosis is responsible for almost 20% of AIDS-related deaths, and in countries like Peru, its incidence remains high, mostly due to the annual increase in new cases of HIV infection. This study aimed to establish the genotypic diversity and antifungal susceptibility of C. neoformans isolates causing meningoencephalitis in 25 adults and a 9-year-old girl with HIV and other risk factors from Lima, Peru. To identify the genotype of the isolates, multilocus sequence typing was applied, and to establish the susceptibility of the isolates to six antifungals, a YeastOne® broth microdilution was used. From the isolates, 19 were identified as molecular type VNI, and seven as VNII, grouped in eight and three sequence types, respectively, which shows that the studied population was highly diverse. Most isolates were susceptible to all antifungals tested. However, VNI isolates were less susceptible to fluconazole, itraconazole and voriconazole than VNII isolates (p < 0.05). This study contributes data on the molecular epidemiology and the antifungal susceptibility profile of the most common etiological agent of cryptococcosis, highlighting a pediatric case, something which is rare among cryptococcal infection.

show abstract

“…The remaining two isolates were selected for sequencing from the Westmead Medical Mycology Collection, Sydney/Perth, Australia due to their MLST profiles. These were collected from a clinical case in Argentina [7] and a veterinary case in Arizona (Table 1). *The isolate was obtained from a Mexican non-HIV patient living in Spain Genome libraries were prepared with a DNA prep kit (Illumina) at 1/4 th of the reaction volumes and sequenced on a NextSeq 550 (Illumina).…”

Section: Methodsmentioning

confidence: 99%

Phylogenomic Placement of American Southwest-Associated Clinical and Veterinary Isolates Expands Evidence for Distinct <i>Cryptococcus Gattii</i> VGVI.

Monroy-Nieto¹,

Bowers²,

Montfort³

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Whole-genome sequencing has advanced our understanding of the population structure of the pathogenic species complex Cryptococcus gattii, which has allowed for the phylogenomic specification of previously described major molecular type groupings and novel lineages. Recently, isolates collected in Mexico in the 1960s were determined to be genetically distant from other known molecular types and were classified as VGVI. We sequenced four clinical isolates and one veterinary isolate collected in the southwestern U.S. and Argentina during 2012-2021. Phylogenomic analysis groups these genomes with those of the Mexican VGVI isolates, expanding VGVI into a clade and establishing this molecular type as a clinically important population. These findings also potentially expand the known Cryptococcus ecological range with a previously unrecognized endemic area.

show abstract

First case report of cryptococcosis due to Cryptococcus decagattii in a pediatric patient in Argentina

Cited by 11 publications

References 12 publications

Multilocus sequence typing of strains from the Cryptococcus gattii species complex from different continents

Multilocus sequence typing of strains from the Cryptococcus gattii species complex from different continents

Cryptococcus neoformans Causing Meningoencephalitis in Adults and a Child from Lima, Peru: Genotypic Diversity and Antifungal Susceptibility

Phylogenomic Placement of American Southwest-Associated Clinical and Veterinary Isolates Expands Evidence for Distinct <i>Cryptococcus Gattii</i> VGVI.

Contact Info

Product

Resources

About