Alterations induced in the ileum of mice upon inoculation with different species of Leishmania: a preliminary study

Abstract

Leishmania modified the quantity of cells in and morphology of mice ilea.

Infection by Leishmania (Leishmania) infantum chagasi causes intestinal changes B‐1 cells dependent

“…This cytokine contributes to the Paneth cells development, as observed by the increased number of these cells after the adoption of B‐1 cells that resembled those found in the infected wild group. On the other hand, considering the Balb/c I and XID I groups, a reduction in the number of Paneth cells occurred, perhaps due to increased degranulation after infectious conditions …”

“…The thickness of the total ileum wall, muscular and submucosa, the width and depth of the crypts, and height and width of the villi of each animal were measured; 16 images were captured with a 20X objective lens (1 image/quadrant/cut). The Image Pro Plus software (Media Cybernetics) was used to obtain 64 measurements of each parameter …”

“…The Paneth cells were counted in four random crypts per quadrant of four cuts per animal, totalizing 64 crypts. The area of these cells was calculated from 160 Paneth cells randomly chosen in 16 images captured with a 100X objective lens per animal …”

“…Therefore, the goblet cells from each animal were counted along the 2560 epithelial cells. Then, the proportion of these cells in relation to 100 epithelial cells was calculated …”

“…After laparotomy, the ileum was fixed in paraformaldehyde and em- used to obtain 64 measurements of each parameter. [31][32][33] The height and width of 80 enterocytes and their nuclei/animal were measured by capturing images of the intestinal epithelium with a 100X objective lens (1 image/quadrant/cut). The Paneth cells were counted in four random crypts per quadrant of four cuts per animal, totalizing 64 crypts.…”