Rapid Detection of Sepsis using CESDA: the Caenorhabditis elegans Sepsis Detection Assay

Tee, Ling Fei; Tan, Toh Leong; Neoh, Hui Min; Jamal, A. Rahman A.

doi:10.1590/0037-8682-0300-2018

Cited by 3 publications

(2 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The potential for animals to sense malignancy has received increasing attention since the phenomenon was first described over three decades ago (Williams and Pembroke, 1989). Since the first report of C. elegans accurately classifying cancer samples by Hirotsu et al (2015), similar methods have been applied using C. elegans to detect sepsis (Tee et al, 2019) and tuberculosisspecific odorants (Neto et al, 2016). Two of the VOCs proposed to be increased in PrCa urine samples by Khalid et al (2015) were found to elicit behavioral attraction of C. elegans under our assay conditions.…”

Section: Discussionmentioning

confidence: 88%

A Caenorhabditis elegans behavioral assay distinguishes early stage prostate cancer patient urine from controls

et al. 2021

View full text Add to dashboard Cite

Current methods for non-invasive prostate cancer (PrCa) detection have a high false-positive rate and often result in unnecessary biopsies. Previous work has suggested that urinary volatile organic compound (VOC) biomarkers may be able to distinguish PrCa cases from benign disease. The behavior of the nematode Caenorhabditis elegans has been proposed as a tool to take advantage of these potential VOC profiles. To test the ability of C. elegans Bristol N2 to distinguish PrCa cases from controls, we performed chemotaxis assays using human urine samples collected from men screened for PrCa. Behavioral response of nematodes towards diluted urine from PrCa cases were compared to controls with cancer-free controls. Overall, we observed a significant attraction of young adult-stage C. elegans nematodes to 1:100 diluted urine from confirmed PrCa cases and repulsion of C. elegans to urine from controls. When C. elegans chemotaxis index was considered alongside prostate-specific antigen levels for distinguishing cancer from cancer-free controls, the accuracy of patient classification was 81%. We also observed behavioral attraction of C. elegans to two previously reported VOCs to be increased in PrCa patient urine. We conclude nematode behavior distinguishes PrCa case urine from controls in a dilution-dependent manner.

show abstract

Section: Discussionmentioning

confidence: 88%

A Caenorhabditis elegans behavioral assay distinguishes early stage prostate cancer patient urine from controls

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Although compounds used here are single odorants and not a complex olfactory signature, the authors discussed the possibility of using automatic tracking to rapidly obtain a diagnosis of tuberculosis (Neto et al, 2016). Another study used the same circular arena apparatus to detect the presence of sepsis in urine of patients, and C. elegans nematodes were shown to be spontaneously attracted to the odour of sick patients (Tee et al, 2019). This attraction/repulsion behaviour in nematodes was also used for testing cancer detection.…”

Section: The Nematode Caenorhabditis Elegansmentioning

confidence: 99%

The neglected potential of invertebrates in detecting disease via olfaction

2023

View full text Add to dashboard Cite

Agents that cause disease alter the cell metabolism of their hosts. Cells with an altered metabolism produce particular profiles of biomolecules, which are different from those of healthy cells. Such differences may be detected by olfaction. Historically, physicians used olfactory cues to diagnose sickness by smelling the breath or the urine of patients. However, other species have been shown to possess excellent olfactory abilities. Dogs, for instance, have been frequently used as biodetectors of human diseases, including cancer, viral and bacterial infections. Other mammalian species, such as rats, have been trained to perform similar tasks, but their disease detection abilities remain poorly explored. Here, we focus on the overlooked potential of invertebrate species and we review the current literature on olfactory detection of diseases by these animals. We discuss the possible advantages of exploring further the abilities of invertebrates as detection tools for human disease.

show abstract

Organs-on-Chips Platforms Are Everywhere: A Zoom on Biomedical Investigation

et al. 2022

View full text Add to dashboard Cite

Over the decades, conventional in vitro culture systems and animal models have been used to study physiology, nutrient or drug metabolisms including mechanical and physiopathological aspects. However, there is an urgent need for Integrated Testing Strategies (ITS) and more sophisticated platforms and devices to approach the real complexity of human physiology and provide reliable extrapolations for clinical investigations and personalized medicine. Organ-on-a-chip (OOC), also known as a microphysiological system, is a state-of-the-art microfluidic cell culture technology that sums up cells or tissue-to-tissue interfaces, fluid flows, mechanical cues, and organ-level physiology, and it has been developed to fill the gap between in vitro experimental models and human pathophysiology. The wide range of OOC platforms involves the miniaturization of cell culture systems and enables a variety of novel experimental techniques. These range from modeling the independent effects of biophysical forces on cells to screening novel drugs in multi-organ microphysiological systems, all within microscale devices. As in living biosystems, the development of vascular structure is the salient feature common to almost all organ-on-a-chip platforms. Herein, we provide a snapshot of this fast-evolving sophisticated technology. We will review cutting-edge developments and advances in the OOC realm, discussing current applications in the biomedical field with a detailed description of how this technology has enabled the reconstruction of complex multi-scale and multifunctional matrices and platforms (at the cellular and tissular levels) leading to an acute understanding of the physiopathological features of human ailments and infections in vitro.

show abstract

Rapid Detection of Sepsis using CESDA: the Caenorhabditis elegans Sepsis Detection Assay

Cited by 3 publications

References 14 publications

A Caenorhabditis elegans behavioral assay distinguishes early stage prostate cancer patient urine from controls

A Caenorhabditis elegans behavioral assay distinguishes early stage prostate cancer patient urine from controls

The neglected potential of invertebrates in detecting disease via olfaction

Organs-on-Chips Platforms Are Everywhere: A Zoom on Biomedical Investigation

Contact Info

Product

Resources

About