Identification of São Paulo metallo-beta-lactamase-1-producing Pseudomonas aeruginosa in the Central-West region of Brazil: a case study

Maciel, Wirlaine Glauce; Silva, Késia Esther da; Bampi, José Victor Bortolotto; Bet, Graciela Mendonça dos Santos; Ramos, Ana Luísa; Gales, Ana Cristina; Simionatto, Simone

doi:10.1590/0037-8682-0284-2016

Cited by 3 publications

(2 citation statements)

References 11 publications

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“…The data in Figure 3 show controversial results regarding the risk of death among patients infected with SPM-1producing strains, as seen in the three studies that investigated this aspect 2,10,21 . Our database search identified many studies that lacked information regarding the detection of MBL (blaSPM-1) and mortality, preventing a more concrete analysis; with the increasing prevalence of SPM-1-producing strains in different regions, particularly in Brazil, this demonstrates the need for investigations with representative sample sizes for analysis 5,8,10,14,15 .…”

Section: Discussionmentioning

confidence: 99%

“…A relevant aspect regarding this fast dissemination of resistance traits among P. aeruginosa strains is related to the fact that the resistance genotype may be transferred through mobile genetic elements. The emergence of multidrug-resistant (MDR) strains demonstrates the need for changes in routine laboratory diagnostic procedures, such as the incorporation of the molecular detection of bla-variant genes, particularly blaSPM-1, which is the most prevalent variant in Brazil [13][14][15] . Several studies have reported confl icting results regarding mortality associated with multidrug-resistant Pseudomonas aeruginosa (MDRPA) infections, and few reports have highlighted this association with MBL-producing strains 7,8,10,16,17 .…”

Section: Introductionmentioning

confidence: 99%

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Mortality in patients with multidrug-resistant Pseudomonas aeruginosa infections: a meta-analysis

Matos

Andriolo

Rodrigues

et al. 2018

Rev. Soc. Bras. Med. Trop.

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Pseudomonas aeruginosa is the leading cause of nosocomial infections with high mortality rates owing to the limited therapeutic options for multidrug-resistant Pseudomonas aeruginosa (MDRPA) and metallo-beta-lactamase (MBL)-producing strains. Herein, we present a meta-analysis exploring the association between MDRPA and São Paulo MBL-1 (SPM-1)-producing strains vs. mortality. Online databases were screened to identify studies published between 2006 and 2016. A total of 15 studies, comprising 3,201 cases of P. aeruginosa infection, were included. Our results demonstrated a higher mortality rate among patients infected with MDRPA (44.6%, 363/813) than those with non-MDRPA infection (24.8%, 593/2,388) [odds ratio (OR) 2.39, 95% confidence interval (CI) 1.70-3.36, p <0.00001]. The risk of mortality in patients with non-SPM-1 strains was four times higher than that observed in the patients of the SPM-1 group; however, no statistically significant difference was observed (p = 0.43). In conclusion, the results of our study demonstrated that patients infected with MDRPA had a significantly higher mortality rate than that of patients infected with non-MDRPA strains, especially patients with bloodstream infection (BSI), immunosuppression, and inadequate antimicrobial therapy. The absence of studies on the molecular aspects of blaSPM-1 and its association with mortality limited the analysis; therefore, our results should be interpreted with caution. Our findings also highlight the need for more studies on the molecular aspects of resistance and the peculiarities of different nosocomial settings.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mortality in patients with multidrug-resistant Pseudomonas aeruginosa infections: a meta-analysis

Matos

Andriolo

Rodrigues

et al. 2018

Rev. Soc. Bras. Med. Trop.

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Update on the epidemiology of carbapenemases in Latin America and the Caribbean

García-Betancur

Appel

Esparza³

et al. 2020

Expert Review of Anti-infective Therapy

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In vitro and in vivo antibacterial activity assays of carvacrol: A candidate for development of innovative treatments against KPC-producing Klebsiella pneumoniae

et al. 2021

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Dissemination of carbapenem-resistant Klebsiella pneumoniae poses a threat to the successful treatment of bacterial diseases and increases the need for new antibacterial agents development. The objective of this study was to determine the antimicrobial activity of carvacrol against multidrug-resistant K. pneumoniae. Carbapenemase production was detected by MALDI-TOF. The PCR and sequencing showed that the blaKPC-2, blaOXA-48, blaNDM-1, blaCTX-M-8 genes were present in carbapenem-resistant K. pneumoniae strains. The polymyxin-resistant K. pneumoniae strain exhibited alterations in mgrB gene. The antimicrobial activity of carvacrol was evaluated in vitro using broth microdilution and time-kill methods. For this, carbapenem-resistant K. pneumoniae and polymyxin-resistant strains, were evaluated. The in vitro results showed that carvacrol had antimicrobial activity against all isolates evaluated. The survival curves showed that carvacrol eradicated all of the bacterial cells within 4 h. The antimicrobial effect of carvacrol in vivo was determined using a mouse model of infection with Klebsiella pneumoniae carbapenemase (KPC). The treatment with carvacrol was associated with increased survival, and significantly reduced bacterial load in peritoneal lavage. In addition, groups treated with carvacrol, had a significant reduction in the total numbers of white cell and significantly increased of platelets when compared to the untreated group. In vivo and in vitro studies showed that carvacrol regimens exhibited significant antimicrobial activity against KPC-producing K. pneumoniae, making it an interesting candidate for development of alternative treatments.

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Identification of São Paulo metallo-beta-lactamase-1-producing Pseudomonas aeruginosa in the Central-West region of Brazil: a case study

Cited by 3 publications

References 11 publications

Mortality in patients with multidrug-resistant Pseudomonas aeruginosa infections: a meta-analysis

Mortality in patients with multidrug-resistant Pseudomonas aeruginosa infections: a meta-analysis

Update on the epidemiology of carbapenemases in Latin America and the Caribbean

In vitro and in vivo antibacterial activity assays of carvacrol: A candidate for development of innovative treatments against KPC-producing Klebsiella pneumoniae

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