Neospora caninum and Toxoplasma gondii serodiagnosis in human immunodeficiency virus carriers

Oshiro, Leandra Marla; Motta-Castro, Ana Rita Coimbra; Freitas, Solange Zacalusni; Cunha, Rodrigo Casquero; Dittrich, Rosângela Locatelli; Meirelles, Andréa Christine Ferreira; Andreotti, Renato

doi:10.1590/0037-8682-0151-2015

Cited by 32 publications

(32 citation statements)

References 21 publications

(24 reference statements)

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“…Dogs and related canids (Coyotes or foxes) are definitive hosts of N. caninum (Dubey et al., ). Low antibody titres to N. caninum have also been reported in humans (Lobato et al, ; Oshiro et al, ; Tranas, Heinzen, Weiss, & McAllister., ), posing a potential public health problem.…”

Section: Introductionmentioning

confidence: 87%

First report of Neospora caninum infection in pigs in China

Gui

et al. 2019

Transbound Emerg Dis

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Neospora caninum is a protozoan parasite which can infect many mammals and birds with a worldwide distribution. However, no molecular data are available about the occurrence of N. caninum in pigs. In this study, the serological and molecular prevalence of N. caninum infection in farmed pigs were investigated in Hunan province, China, between January 2017 and December 2018. A total of 1,500 serum samples collected from 10 herds in Hunan province were evaluated using a competitive‐inhibition enzyme‐linked immunoassay assay (cELISA). The overall seroprevalence of N. caninum in the examined pigs was 1.9%. The seroprevalence of N. caninum ranged from 0.3% to 4.6% among different regions in Hunan province of China (p < .05). DNA was extracted from brain samples, and the Nc‐5 gene and ITS‐1 region were amplified and then sequenced. Three (0.5%) of the examined 600 brain tissues were found to contain N. caninum DNA. Our phylogenetic analyses indicated that N. caninum samples were classified into two distinct groups. Although the prevalence is low within the pig groups investigated, our results revealed the emergence of N. caninum infection in pigs in China. The finding of the present study provides molecular evidence that the pigs are the natural intermediate host of N. caninum and may have major epidemiological importance.

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Section: Introductionmentioning

confidence: 87%

First report of Neospora caninum infection in pigs in China

Gui

et al. 2019

Transbound Emerg Dis

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“…In the second study, antibodies to N. caninum (IFAT,1:50) were sought in Human Immunodeficiency Virus (HIV) patients; which 26.1% (81/310) patients from MS and 31.2% (10/31) patients from PR were seropositive and one patient had a titer of 1:400 (OSHIRO et al, 2015). In the third report, N. caninum antibodies (IFAT 1:100) were detected in 7 of 67 (10,5%) humans from 24 farms of MT (BENETTI et al, 2009).…”

Section: Clinical Infectionsmentioning

confidence: 99%

All about neosporosis in Brazil

Cerqueira‐Cézar

Calero‐Bernal

Dubey

et al. 2017

Rev. Bras. Parasitol. Vet.

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Neospora caninum is protozoan parasite with domestic and wild dogs, coyotes and grey wolves as the definitive hosts and many warm-blooded animals as intermediate hosts. It was cultivated and named in 1988. Neosporosis is a major disease of cattle and has no public health significance. Since 1990's N. caninum has emerged as a major cause of abortion in cattle worldwide, including in Brazil. N. caninum also causes clinical infections in several other animal species. Considerable progress has been made in understanding the biology of N. caninum and there are more than 200 papers on this subject from Brazil. However, most of the reports on neosporosis from Brazil are serological surveys. Overall, little is known of clinical neosporosis in Brazil, particularly cattle. The few reports pertain to sporadic cases of abortion with no information on epidemics or storms of abortion. The objective of the present review is to summarize all reports from Brazil and suggest topic for further research, including prevalence of N. caninum oocysts in soil or in canine feces, and determining if there are additional definitive hosts, other than the domestic dog. There is need for a national survey in cattle using defined parameters. Future researches should focus on molecular characterization of N. caninum strains, possibility of vaccine production and relationship between wildlife and livestock epidemiology.Keywords: Neospora caninum, neosporosis, domestic animals, wild animals, Brazil. ResumoNeospora caninum é um protozoário parasita que possui os canídeos domésticos e selvagens, coiotes e lobos cinzentos como hospedeiros definitivos e vários animais de sangue quente como hospedeiros intermediários. Foi cultivado e nomeado em 1988. A neosporose é uma das principais doenças em bovinos e não tem significância em saúde pública. Desde 1990, N. caninum tem emergido como uma das principais causas de aborto em bovinos em todo o mundo, inclusive no Brasil. N. caninum também causa infecções clínicas em várias outras espécies animais. Consideráveis avanços foram feitos na compreensão da biologia desse parasita e há mais de 200 trabalhos sobre o assunto no Brasil. No entanto, a maioria dos relatos de neosporose do Brasil são relacionados a sorologia. Em geral, pouco se sabe sobre a neosporose clínica no Brasil, particularmente em bovinos. Os poucos relatos referem-se a casos esporádicos de aborto sem informações sobre epidemias ou surtos de aborto. O objetivo da presente revisão é resumir todos os relatos sobre N. caninum no Brasil e sugerir tópicos para pesquisas futuras, incluindo a prevalência de oocistos de N. caninum no solo ou em fezes caninas e determinar se há hospedeiros definitivos adicionais, exceto o cão doméstico no país. Uma pesquisa nacional em bovinos usando parâmetros definidos seria de grande importância. Pesquisas futuras deveriam ser focadas na caracterização de cepas de N. caninum, possibilidade de produção de vacinas e a relação epidemiológica entre a vida selvagem e o gado.Palavras-chave: Neospora caninum, neospo...

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“…contrast, N. caninum is a major cause of neonatal mortality and/or abortion in ruminants and neuromuscular disorders in dogs (Boas et al, 2015;Donahoe et al, 2015). There are no reports of human infection with N. caninum, whereas inoculation of pregnant monkeys with N. caninum results in transplacental transmission of the parasite and the induction of fetal encephalitis (Dubey, 2003;McCann et al, 2008;Oshiro et al, 2015). In keeping with this, the potential of N. caninum to infect humans is unknown (Dubey, 2003;McCann et al, 2008;Oshiro et al, 2015).…”

mentioning

confidence: 99%

“…Contrariwise, the only established definitive hosts of N. caninum are dogs, gray wolfs, coyotes, and dingoes that causes a serious disease particularly in ruminants (Belluco et al, 2017;Boas et al, 2015;Donahoe et al, 2015;Dubey, 2003;Hussain et al, 2017;Jones & Dubey, 2012;Klauck et al, 2016;McCann et al, 2008;Oshiro et al, 2015). Recorded data showed that intermediate hosts of T. gondii and N. caninum become infected in two ways of vertical (transplacental) and horizontal (ingestion of oocysts shed in milk and meat) (Belluco et al, 2017;Boas et al, 2015;Donahoe et al, 2015;Dubey, 2003;Hussain et al, 2017;Jones & Dubey, 2012;Klauck et al, 2016;McCann et al, 2008;Oshiro et al, 2015). Thus, contaminated milk and dairy products (particularly traditional dairy products) play an imperative role in the distribution of T. gondii and N. caninum infections to human (Ayinmode, Akinseye, Schares, & Cadmus, 2017;Gos et al, 2017).…”

mentioning

confidence: 99%

Retracted: Prevalence of Toxoplasma gondii and Neospora caninum in different types of raw milk and traditional dairy product samples

Alipour

Rahimi

Shakerian

2018

Journal of Food Safety

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The current investigation was done to examine the prevalence of Toxoplasma gondii and Neospora caninum in raw milk and traditional dairy samples. Eight‐hundred and eighty samples were collected and subjected to DNA extraction and nested PCR for detection of T. gondii B1 and N. caninum Nc5 genes. Seventy out of 880 (7.95%) and 80 out of 880 (10%) samples were positive for T. gondii and N. caninum, respectively. Simultaneous prevalence of T. gondii and N. caninum in studied samples was 4.20%. Prevalence of T. gondii and N. caninum in raw milk and traditional dairy samples were 6% and 10.60% and 10.52% and 9.21%, respectively. Traditional cheese and butter (15.00%) had the highest prevalence of T. gondii. Raw bovine (24.00%) had the highest prevalence of N. caninum. Boost prevalence of parasites in samples revealed an important public health threat regarding the consumption of raw milk and traditional dairy. Practical applications Due to the high consumption rate of milk and dairy products, they should have a high microbial quality. Toxoplasma gondii and Neospora caninum are two closely related protozoa with considerable zoonotic importance regarding the consumption of raw milk and traditional dairy products. The current research represents that raw milk and traditional dairy samples may be the sources of T. gondii and N. caninum transmission into the human population.

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Neospora caninum and Toxoplasma gondii serodiagnosis in human immunodeficiency virus carriers

Cited by 32 publications

References 21 publications

First report of Neospora caninum infection in pigs in China

First report of Neospora caninum infection in pigs in China

All about neosporosis in Brazil

Retracted: Prevalence of Toxoplasma gondii and Neospora caninum in different types of raw milk and traditional dairy product samples

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