Expression of co-stimulatory molecules CD80 and CD86 is altered in CD14 + HLA-DR + monocytes from patients with Chagas disease following induction by Trypanosoma cruzi recombinant antigens

Abstract

Introduction:The relationships between monocytes and lymphocytes through MHC class II molecules and costimulatory, are of utmost importance for the production of an efficient immune response. In this work, we assessed the expression of surface molecules CD80 and CD86 on CD14 + HLA-DR + monocytes from patients with Chagas disease. Methods: The study population consisted of 31 patients with chronic clinical forms of Chagas disease. Patient blood samples were cultured in the presence of recombinant cytoplasmic repetitive antigen (CRA) and flagellar repetitive antigen (FRA). Results: We found considerable differences in the expression profile of surface molecules involved in antigen presentation. Conclusions: CRA and FRA may contribute to host immune response evasion by Trypanozoma cruzi.

CD86 Expression by Monocytes Influences an Immunomodulatory Profile in Asymptomatic Patients with Chronic Chagas Disease

“…The immune response mediated by CD4 + T lymphocytes is highly important for the development of Chagas disease involving the Th1, Th2, Th17, or Treg CD4 + lymphocytes that differ in their functions, secreted cytokine pattern, and specific expression of transcription factors ( 16 , 43 ). Therefore, we evaluated the frequency of Th1, Th2, Th17, and Treg lymphocytes in the peripheral blood following in vitro stimulation with T. cruzi antigens (Figure 6 B).…”

“…We propose that CD80 expression may be involved in the activation of T cells in Chagas patients, while CD86 might be associated with the inhibition of them, downmodulating the responses of effector T cells in asymptomatic patients. Previous studies have shown that exposure of monocytes to T. cruzi leads to an increase in the frequency of CD80 by monocytes of IND and CARD patients ( 43 ), whereas CD86 expression is decreased in CARD but not in IND patients ( 35 ). We suggest that these co-stimulatory molecules have opposing functions and probably are a key role in the T cells activation in Chagas disease.…”

“…CD80 and CD86 co-stimulatory molecules in monocytes from patients with Chagas disease following induction by T. cruzi recombinant antigens are important for the activation of T cells ( 43 ). However, no study to date has demonstrated the expression of these molecules in the different monocytes subsets in Chagas disease.…”

Type 2 diabetes mellitus metabolic control correlates with the phenotype of human monocytes and monocyte-derived macrophages

“…Aside from circulating monocytes, we carried out the cell-surface marker assessment in our MDM model using M-CSF and autologous human sera to mimic the diabetic's blood microenvironment, finding also a decrease in HLA-DR, CD86, and CD163, which negatively correlated with glycemic or metabolic control parameters. Interestingly, HLA-DR is expressed by antigen-presenting cells, such as monocytes and macrophages, where this molecule stimulates adaptive immune response by presenting peptides to T lymphocytes, and CD86 is a costimulatory molecule that promotes T lymphocyte activation and differentiation, suggesting that the process of antigen presentation may be affected in T2D patients (29). In agreement with our results, a decrease of mRNA for HLA-DR in whole blood was documented in obese adolescents with insulin resistance, compared to obese and to healthy subjects without signs of insulin resistance (30).…”

The Role of Co-Stimulatory Molecules in Chagas Disease

“…On the other hand, a recent study also demonstrated an increase in CD80 expression in the total monocytes of IND and CARD patients and a reduction in CD86 expression in CARD individuals after being stimulated with cytoplasmic repetitive and flagellar repetitive T. cruzi antigens [ 151 ]. Moreover, a higher CD80 expression in classical monocytes of IND and CARD patients was seen, but only IND patients showed a greater CD86 expression in total, classical, intermediate, and non-classical monocytes as well as higher CD86 rather than CD80 expression [ 49 ].…”