Larvicidal and adulticidal effects and ultrastructural changes of larvae midgut epithelium of Musca domestica (Diptera: Muscidae) fed with Bacillus thuringiensis var. kyushuensis

Abstract

Introduction: Musca domestica is resistant to many insecticides; hence, biological control is a suitable alternative. Methods: We evaluated the lethality of strain Btk176 towards the larval and adult M. domestica and the histopathological effects in the larvae midgut. Results: We observed 99% larval and 78.9% adult mortality within 48 hours of spore ingestion (dosage, 2.4×10 8 CFU/ml). The histopathological effects were consistent with cytotoxicity. PCR analysis showed the presence of the cry1Ba gene. Transmission electron microscopy revealed a bipyramidal parasporal body. Thurigiensin activity was not detected. Conclusions: The serovar, Btk176 might be a potential biocontrol agent for houseflies.

Entomopathogenic potential of Bacillus subtilis and Bacillus amyloliquefaciens strains against Musca domestica under controlled conditions

“…Bacillus amyloliquefaciens CBMDLO3 differentiated from the remaining strains, as its CS achieved a 5‐fold reduction of larvae and pupae survival (to approximately 30% survival in both stages), compared to the 90% survival of the control. These entomopathogenic activity results agree with those reported for other bacterial strains (Khurshed & Shakoori, 2005; Ruiu et al, 2011; Zimmer et al, 2013; Pereira et al, 2019). Zimmer et al (2013) evaluated various bacterial strains with entomopathogenic activity [ Brevibacillus laterosporus (Laubach) (Bl), B. thuringiensis var.…”

“…Bacillus thuringiensis Berliner is distinguished from other species of Bacillus by the presence of a parasporal inclusion (Lacey, 2017). During development and/or sporulation, B. thuringiensis produces toxins (β‐exotoxin and δ‐endotoxin) with activity against insects belonging to Diptera and Lepidoptera (Faten et al, 2011; Merdan, 2012; Pereira et al, 2019). On the other hand, Lysinibacillus sphaericus Ahmed et al (formerly called Bacillus sphaericus ) produces a toxin that has been used effectively for the biological control of various mosquito species, vectors of important pathogens that cause diseases in humans and animals (Monnerat et al, 2004; Park et al, 2005; Glare et al, 2017).…”

Ultrastructural and pathogenicity of Brevibacillus laterosporus against sinantropic muscoid dipterans

“…Bioassays were performed as reported by Carramaschi, de Pereira, de Queiroz, and Zahner (2015) for C. putoria and C. mecagephala, starting with 10 newly hatched larvae, transferred to plastic pots containing 5 g of diet, which consisted of rotting ground beef mixed with 3 ml of bacterial suspension. Bioassays with M. domestica were performed as reported by de Andrade Pereira et al (2019). As such spore suspension (1 ml) was mixtured with 2.5 g of diet [putrefied ground beef with wheat bran (1:0.5)] and put into individual plastic cups containing 10 neo larvae.…”

Untitled

“…Комнатная муха может переносить возбудителей респираторных инфекций крупного рогатого скота, что наносит большой ущерб многим животноводческим комплексам (Pereira et al, 2019), а также играет важную роль в распространении спор грибов родов Aspergillus, Fusarium, Показано, что комнатная муха может переносить возбудителей болезней, связанных с цирковирусом свиней, который с начала 1990-х годов стал серьезной проблемой в свиноводстве всего мира. Основные вспышки этой болезни в Северной Америке и Европе были связаны с появлением штамма цирковируса свиней 2b (PCV2b) (Blunt et al, 2011).…”