First report of TEM-104-, SHV-99-, SHV-108-, and SHV-110-producing Klebsiella pneumoniae from Iran

Abstract

Introduction: Extended-spectrum beta-lactamases (ESBLs) are bacterial enzymes capable of hydrolyzing beta-lactams. The aim of this study was to describe the prevalence of TEM-and SHV-type ESBL-producing Klebsiella pneumoniae strains in Zahedan, Southeast Iran. Methods: A total of 170 non-repetitive K. pneumoniae strains were collected from patients referred to three teaching hospitals of Zahedan. Antibiotic susceptibility testing was determined for 17 antibiotics using the Kirby-Bauer disc diffusion method. The frequency of ESBL-producing strains was calculated, and minimum inhibitory concentrations of ESBLproducing strains were determined for cefotaxime, ceftazidime, ceftriaxone, and cefpodoxime. The presence of bla TEM and bla SHV genes was tested in all ESBL-producing strains using polymerase chain reaction and DNA sequencing. Results: Among the 170 K. pneumoniae clinical isolates, 55 (32.4%) were ESBL producers; 92.7% (n=51) and 72.7% (n=40) of the isolates carried the bla SHV and bla TEM genes, respectively, and 67.3% (n=37) carried both genes. The sequencing results showed that all bla TEM types were bla TEM-1 , except for two isolates that were bla . The bla SHV types were bla SHV-1 , bla SHV-11 , bla , bla SHV-99 , bla , and bla . Conclusions: The percentage of bla TEM and bla SHV among ESBL-producing K. pneumoniae isolates from Zahedan is relatively high, indicating the need for further surveillance and consideration in antibiotic use. To the best of our knowledge, this is the fi rst report of TEM-104-, SHV-99-, SHV-108-, and SHV-110-type ESBLs among clinical isolates of K. pneumoniae from Iran, and TEM-1, SHV-1, SHV-11, and SHV-12 appear to be the dominant ESBLs in this region.

High rate of carbapenem-resistant Klebsiella pneumoniae detected from hospital equipments in Iran

“…To the best of our knowledge, this is the second report of bla IMP -harbored K. pneumoniae from Iran after Khorvash et al [27]. This study showed a high prevalence of ESBLs-encoding genes (bla SHV , bla CTX-M , and bla TEM ) among CRKP isolates, which is consistent with other studies conducted in Isfahan [25,26] and Iran [28][29][30]. However, we have not detected bla KPC , bla GES , bla IMI , and bla VIM among CRKP isolates.…”

High Frequency of qnr Genes in Urinary Isolates of Extended-Spectrum β-Lactamase (ESBL)-producing Klebsiella pneumoniae in Tehran, Iran

“…ESBL-producing K. pneumoniae isolates are of great concern, both in treatment and in management of nosocomial infections. As multiple resistance genes mostly reside The frequency of ESBL-producing K. pneumoniae isolates was higher in this study than in some previous Iranian studies (16)(17)(18). However, higher frequencies were reported by Mahmoudi et (19,20).…”

Molecular detection of blaTEM, blaSHV,and blaCTX-M genes among Uropathogenic Escherichia coli isolated from cases with urinary tract infection in Erbil city-Iraq

“…In addition, the present study demonstrated that the blaTEM type β-lactamase gene was the most prevalent ESBL gene in UPEC, which is consistent with the findings of several previously published studies (27)(28)(29)(30). In Italy (45.4%) [31], Portugal (40.9%) [32], and Turkey (72.7%), the blaTEM type βlactamase gene was the most prevalent [33].…”