Relationships between phagocytosis, mucoid phenotype, and genetic characteristics of Klebsiella pneumoniae clinical isolates

Cavalcanti, Francinete Carla Nunes; Rodrigues, Juliana Falcão; Cabral, Adriane Borges; Azarias, Larissa Caina Barbosa de; Júnior, Marcos Antônio de Morais; Castro, Célia Maria Machado Barbosa de; Lópes, Ana Catarina de Souza

doi:10.1590/0037-8682-0089-2019

Cited by 4 publications

(4 citation statements)

References 14 publications

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“…In vitro phagocytosis assays provided insight into the potential of the nasal isolates to survive this first barrier of cell immunity in the lung. Heterogeneity in this feature has been shown for other bacterial species, such as H. parasuis [14] or Klebsiella pneumoniae [22]. In our case, strains with high association to PAMs, such as LG6-2, were observed, while other strains did not associate with the phagocytic cells, as the case of the virulent SN9-4M.…”

Section: Discussionsupporting

confidence: 71%

Heterogeneity of Moraxella isolates found in the nasal cavities of piglets

López-Serrano¹,

Galofré-Milà²,

Costa-Hurtado³

et al. 2019

Preprint

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Previous studies have shown that the genus Moraxella is commonly present in the nasal microbiota of swine. In this study, 51 isolates of Moraxella were obtained from nasal swabs from 3-4 week old piglets, which represented 26 different fingerprintings by enterobacterial repetitive intergenic consensus (ERIC)-PCR. Whole 16S rRNA gene sequencing allowed the identification at species level of the Moraxella spp. isolates. The majority of the field strains were identified as Moraxella pluranimalium, but Moraxella porci was also detected. In addition, a cluster of 7 strains did not group with any described Moraxella species, probably representing a new species. Subsequent phenotypic characterization indicated that strains of Moraxella pluranimalium were mainly sensitive to the serum complement, while the cluster representing the putative new species was highly resistant. Biofilm formation capacity was very variable among the Moraxella spp. isolates, while adherence to epithelial cell lines was similar among selected strains. Additionally, selected strains were tested in phagocytosis assays and again variability was observed in the susceptibility to alveolar macrophages. Antimicrobial tests evidenced the existence of multidrug-resistance in the strains. In summary, phenotypic characterization revealed heterogeneity among Moraxella strains from the nasal cavity of piglets. Strains with pathogenic potential were detected as well as those that may be commensal members of the nasal microbiota. However, the role of Moraxella in porcine diseases and health should be further evaluated.

show abstract

Section: Discussionsupporting

confidence: 71%

Heterogeneity of Moraxella isolates found in the nasal cavities of piglets

López-Serrano¹,

Galofré-Milà²,

Costa-Hurtado³

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 71%

Heterogeneity of Moraxella isolates found in the nasal cavities of piglets

López-Serrano¹,

Galofré-Milà²,

Costa-Hurtado³

et al. 2019

Preprint

View full text Add to dashboard Cite

Background: Previous studies have shown that the genus Moraxella is commonly present in the nasal microbiota of swine. Results: In this study, 51 isolates of Moraxella were obtained from nasal swabs from 3-4 week old piglets, which represented 26 different fingerprintings by enterobacterial repetitive intergenic consensus (ERIC)-PCR. Whole 16S rRNA gene sequencing allowed the identification at species level of the Moraxella spp. isolates. The majority of the field strains were identified as Moraxella pluranimalium, but Moraxella porci was also detected. In addition, a cluster of 7 strains did not group with any described Moraxella species, probably representing a new species. Subsequent phenotypic characterization indicated that strains of Moraxella pluranimalium were mainly sensitive to serum complement, while the cluster representing the putative new species was highly resistant. Biofilm formation capacity was very variable among the Moraxella spp. isolates, while adherence to epithelial cell lines was similar among selected strains. Additionally, variability was also observed in the association of selected strains to porcine alveolar macrophages. Antimicrobial tests evidenced the existence of multidrug-resistance in the strains. Conclusions : In summary, phenotypic characterization revealed heterogeneity among Moraxella strains from the nasal cavity of piglets. Strains with pathogenic potential were detected as well as those that may be commensal members of the nasal microbiota. However, the role of Moraxella in porcine diseases and health should be further evaluated.

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“…In vitro assays with PAMs provided insight into the potential of the nasal isolates to survive this first barrier of cell immunity in the lung. Heterogeneity in this feature has been shown for other bacterial species, such as H. parasuis [17] or Klebsiella pneumoniae [18]. In our case, strains with high association to PAMs, such as LG6-2, were observed, while other strains did not associate with the phagocytic cells, as the case of the virulent SN9-4 M. According to the results, macrophage association is not directly associated with the phylogeny of the isolates, since heterogeneity in this trait was observed within the distinct species or clusters.…”

Section: Discussionmentioning

confidence: 85%

Heterogeneity of Moraxella isolates found in the nasal cavities of piglets

et al. 2020

View full text Add to dashboard Cite

Background: Previous studies have shown that the genus Moraxella is commonly present in the nasal microbiota of swine. Results: In this study, 51 isolates of Moraxella were obtained from nasal swabs from 3 to 4 week old piglets, which represented 26 different fingerprintings by enterobacterial repetitive intergenic consensus (ERIC)-PCR. Whole 16S rRNA gene sequencing allowed the identification at species level of the Moraxella spp. isolates. The majority of the field strains were identified as Moraxella pluranimalium, but Moraxella porci was also detected. In addition, a cluster of 7 strains did not group with any described Moraxella species, probably representing a new species. Subsequent phenotypic characterization indicated that strains of Moraxella pluranimalium were mainly sensitive to serum complement, while the cluster representing the putative new species was highly resistant. Biofilm formation capacity was very variable among the Moraxella spp. isolates, while adherence to epithelial cell lines was similar among selected strains. Additionally, variability was also observed in the association of selected strains to porcine alveolar macrophages. Antimicrobial tests evidenced the existence of multidrug-resistance in the strains. Conclusions: In summary, phenotypic characterization revealed heterogeneity among Moraxella strains from the nasal cavity of piglets. Strains with pathogenic potential were detected as well as those that may be commensal members of the nasal microbiota. However, the role of Moraxella in porcine diseases and health should be further evaluated.

show abstract

Relationships between phagocytosis, mucoid phenotype, and genetic characteristics of Klebsiella pneumoniae clinical isolates

Cited by 4 publications

References 14 publications

Heterogeneity of Moraxella isolates found in the nasal cavities of piglets

Heterogeneity of Moraxella isolates found in the nasal cavities of piglets

Heterogeneity of Moraxella isolates found in the nasal cavities of piglets

Heterogeneity of Moraxella isolates found in the nasal cavities of piglets

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