Germination and fungal infection of wild celery (Apium graveolens L.) seeds, from southern Brazil, under different temperature and disinfection conditions

Doncato, Kennia Brum; Costa, César Serra Bonifácio

doi:10.1590/0034-737x201966050010

Cited by 3 publications

(1 citation statement)

References 14 publications

(19 reference statements)

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“…After this process, disinfected seeds, asepsis with 1% sodium hypochlorite for 3 min (Mahajan et al, 2018;Doncato and Costa, 2019), were put in direct contact with the fungal colonies and kept in a growth chamber under the same conditions described above for 24 h. The disinfection of the seeds before inoculation of the fungus allowed to generate information without the possible influence of other microorganisms present in the seed or associated with it through the air or soil during its production in the field. A group of disinfected seeds was separated to form a control (healthy seeds).…”

Section: Fungal Isolation Inoculation and Incubationmentioning

confidence: 99%

Fungal identification in peanuts seeds through multispectral images: Technological advances to enhance sanitary quality

Sudki

Oliveira²,

Medeiros³

et al. 2023

Front. Plant Sci.

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The sanitary quality of seed is essential in agriculture. This is because pathogenic fungi compromise seed physiological quality and prevent the formation of plants in the field, which causes losses to farmers. Multispectral images technologies coupled with machine learning algorithms can optimize the identification of healthy peanut seeds, greatly improving the sanitary quality. The objective was to verify whether multispectral images technologies and artificial intelligence tools are effective for discriminating pathogenic fungi in tropical peanut seeds. For this purpose, dry peanut seeds infected by fungi (A. flavus, A. niger, Penicillium sp., and Rhizopus sp.) were used to acquire images at different wavelengths (365 to 970 nm). Multispectral markers of peanut seed health quality were found. The incubation period of 216 h was the one that most contributed to discriminating healthy seeds from those containing fungi through multispectral images. Texture (Percent Run), color (CIELab L*) and reflectance (490 nm) were highly effective in discriminating the sanitary quality of peanut seeds. Machine learning algorithms (LDA, MLP, RF, and SVM) demonstrated high accuracy in autonomous detection of seed health status (90 to 100%). Thus, multispectral images coupled with machine learning algorithms are effective for screening peanut seeds with superior sanitary quality.

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Section: Fungal Isolation Inoculation and Incubationmentioning

confidence: 99%