A comprehensive analysis of the angiogenesis-related genes in glioblastoma multiforme vs. brain lower grade glioma

Abstract

Brain tumors are one of the most common causes of cancer-related deaths around the world. Angiogenesis is critical in high-grade malignant gliomas, such as glioblastoma multiforme. Objective: The aim of this study is to comparatively analyze the angiogenesis-related genes, namely VEGFA, VEGFB, KDR, CXCL8, CXCR1 and CXCR2 in LGG vs. GBM to identify molecular distinctions using datasets available on The Cancer Genome Atlas (TCGA). Methods: DNA sequencing and mRNA expression data for 514 brain lower grade glioma (LGG) and 592 glioblastoma multiforme (GBM) patients were acquired from The Cancer Genome Atlas (TCGA), and the genetic alterations and expression levels of the selected genes were analyzed. Results: We identified six distinct KDR mutations in the LGG patients and 18 distinct KDR mutations in the GBM patients, including missense and nonsense mutations, frame shift deletion and altered splice region. Furthermore, VEGFA and CXCL8 were significantly overexpressed within GBM patients. Conclusions: VEGFA and CXCL8 are important factors for angiogenesis, which are suggested to have significant roles during tumorigenesis. Our results provide further evidence that VEGFA and CXCL8 could induce angiogenesis and promote LGG to progress into GBM. These findings could be useful in developing novel targeted therapeutics approaches in the future.

Low-Dose Apatinib Improves the Prognosis of Patients with Recurrent High-Grade Gliomas

“…The neovascularization of HGGs is usually more distorted, forming vascular spheres that apparently lack tight junctions and complete pericyte coverage [ 6 ]. Since angiogenesis is a biologically important feature of HGGs, targeted treatment strategies have their advantages [ 7 ]. There are guidelines and several studies using the antiangiogenic drug bevacizumab in the treatment of both primary and recurrent HGGs, all of which have shown good efficacy, further affirming the value of antiangiogenic therapy [ 8 ].…”

“…e neovascularization of HGGs is usually more distorted, forming vascular spheres that apparently lack tight junctions and complete pericyte coverage [6]. Since angiogenesis is a biologically important feature of HGGs, targeted treatment strategies have their advantages [7].…”

Construction and validation of an angiogenesis-related gene expression signature associated with clinical outcome and tumor immune microenvironment in glioma

“…Numerous studies indicate that aberrant angiogenesis is involved in the processes of tumorigenesis, development, invasion, and poor prognosis in glioma ( Tan et al, 2018 ). To date, there are still few studies on ARG in glioma ( Biterge-Sut, 2020 ; Wang et al, 2022 ). Two major aspects of glioma biological processes that contribute to treatment resistance are abnormal formation of new blood vessels via angiogenesis and invasion of glioma cells along white-matter tracts ( Carmeliet, 2005 ; Onishi et al, 2011 ).…”

10×Genomics Single-cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber

“…CXCL8 is an important factor in angiogenesis, which is thought to play an important role in the process of tumorigenesis. CXCL8 could induce angiogenesis 46 . CXCL8 is a proin ammatory chemokine that mediates its signaling through extracellular binding to two G-protein-coupled receptors, C-X-C chemokine receptor type 1 (CXCR1) and C-X-C chemokine receptor type 2 (CXCR2) 47 .…”