Beta-1,3-glucanase inhibitors in Brazilian brown seaweed

Ferreira, Tainá Neves; Barufi, José Bonomi; Horta, Paulo Antunes; Castro, Daniele P.; Genta, Fernando Ariel

doi:10.1590/0001-3765202120191402

Cited by 3 publications

(1 citation statement)

References 20 publications

(14 reference statements)

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“…The brown algae species Lobophora sp., Colpomenia sinuosa (Mertens ex Roth) Derbès & Solier, Padina gymnospora (Kützing) Sonder, and Dictyota sp. inhibit the activity of digestive enzymes like catalase, superoxide dismutase, and glutathione peroxidase [ 27 ]. The literature has also revealed that tannins decrease the activity of the enzymes involved in detoxification, acetylcholine esterase, digestion, and protein synthesis in insects [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Insecticidal Activity of Tannins from Selected Brown Macroalgae against the Cotton Leafhopper Amrasca devastans

Petchidurai,

Sahayaraj,

Al-Shuraym

et al. 2023

Plants

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Seaweeds, also known as marine macroalgae, are renewable biological resources that are found worldwide and possess a wide variety of secondary metabolites, including tannins. Drifted brown seaweed (DBSW) is particularly rich in tannins and is regarded as biological trash. The cotton leaf hopper Amrasca devastans (Distant) has caused both quantitative and qualitative losses in cotton production. Drifted brown seaweeds (DBSWs) were used in this study to extract, qualitatively profile, and quantify the levels of total tannins, condensed tannins, hydrolyzable tannins, and phlorotannins in the seaweeds; test their insecticidal activity; and determine the mechanism of action. The largest amount of tannin extract was found in Sargassum wightii Greville (20.62%) using the Soxhlet method (SM). Significantly higher amounts of hydrolyzable tannins (p = 0.005), soluble phlorotannins (p = 0.005), total tannins in the SM (p = 0.003), and total tannins in the cold percolation method (p = 0.005) were recorded in S. wightii. However, high levels of condensed tannins (CTAs) were observed in Turbinaria ornata (Turner) J. Agardh (p = 0.004). A. devastans nymphs and adults were examined for oral toxicity (OT) and contact toxicity (CT) against DBSW tannin crude extract and column chromatographic fractions 1 (Rf = 0.86) and 2 (Rf = 0.88). Stoechospermum polypodioides (J.V. Lamouroux) J. Agardh crude tannin was highly effective against A. devastans using the OT method (LC50, 0.044%) when compared with the standard gallic acid (LC50, 0.044%) and tannic acid (LC50, 0.122%). Similarly, S. wightii fraction 2 (LC50, 0.007%) showed a greater insecticidal effect against A. devastans adults in OT than gallic acid (LC50, 0.034%) and tannic acid (LC50, 0.022%). The mechanism of action results show that A. devastans adults treated with crude tannin of T. ornata had significantly decreased amylase, protease (p = 0.005), and invertase (p = 0.003) levels when compared with the detoxification enzymes. The levels of glycosidase, lactate dehydrogenase, esterase, lipase, invertase, and acid phosphate activities (p = 0.005) of S. wightii were reduced when compared with those of the Vijayneem and chemical pesticide Monocrotophos. In adult insects treated with LC50 concentrations of S. wightii tannin fraction 1, the total body protein (9.00 µg/µL) was significantly reduced (OT, LC50—0.019%). The SDS-PAGE analysis results also show that S. wightii tannin fraction 1 (OT and CT), fraction 2 (OT), and S. polypodioides fraction 2 (CT) had a significant effect on the total body portion level, appearance, and disappearance of some proteins and polypeptides. This study shows that the selected brown macroalgae can be utilized for the safer management of cotton leaf hoppers.

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Section: Introductionmentioning

confidence: 99%

Insecticidal Activity of Tannins from Selected Brown Macroalgae against the Cotton Leafhopper Amrasca devastans

Petchidurai,

Sahayaraj,

Al-Shuraym

et al. 2023

Plants

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Evaluation of antifungal activity, mechanisms of action and toxicological profile of the synthetic amide 2-chloro- N -phenylacetamide

Ferreira

Cordeiro

Silva

et al. 2023

Drug and Chemical Toxicology

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Induction of wheat resistance by Streptomyces sp. HU2014 strain

Zhu

Рожкова

2023

qpp

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Goal. To determine changes in the activity of enzymes (POD, PAL and GLU) of Triticum aestivum when Streptomyces sp. HU2014, Rhizoctonia cerealis G11 are introduced into the soil and their simultaneous application. Methods. Laboratory: cultivation of microorganisms and wheat plants, introduction of actinomycetes and fungus into the soil. Physico-chemical: POD, PAL and GLU colorimetry. Analytical and mathematical: analysis of the obtained results and their statistical comparison. Results. Changes in the activity of enzymes (POD, PAL and GLU) were noted in the leaves of wheat plants at different time intervals, compared to the control variant. The change manifested mostly in the increase of their activity. The maximum amount of enzyme activity was noted on one variety ZM22: on the third day of POD when inoculated according to the CKP scheme (15762.69 U/g) and GLU when introducing the microorganism into the soil according to the PF3 scheme (28.45 U/g); on the fourth day of the PAL study according to the treatment scheme PF3 (29.37 U/g). The induction of resistance was also determined by the wheat variety. Conclusions. The activity of POD, PAL and GLU was determined by the plant treatment scheme, time period and genotype of the crop. In most cases, during soil treatment with microorganisms, the activity of all three studied enzymes from the leaves of three varieties of wheat increased in different periods of time, compared to the control. The activity of PAL enzyme increased to the greatest extent. It was established that the activity of the POD enzyme was mostly determined by R. cerealis and the dual use of microorganisms, the PAL enzyme by a strain of Streptomyces sp. HU2014, and the GLU enzyme — by inoculation with a phytopathogen and introduction of actinomycetes. That is, changes in the activity of all three enzymes were mostly determined by the strain of Streptomyces sp. HU2014. At the same time, it was shown that R. cerealis G11 mainly induced the defense system of AK58 and ZM22 varieties, and HU2014 induced the defense of BN307.

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Beta-1,3-glucanase inhibitors in Brazilian brown seaweed

Cited by 3 publications

References 20 publications

Insecticidal Activity of Tannins from Selected Brown Macroalgae against the Cotton Leafhopper Amrasca devastans

Insecticidal Activity of Tannins from Selected Brown Macroalgae against the Cotton Leafhopper Amrasca devastans

Evaluation of antifungal activity, mechanisms of action and toxicological profile of the synthetic amide 2-chloro- N -phenylacetamide

Induction of wheat resistance by Streptomyces sp. HU2014 strain

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