Production of Pleurotus sajor-caju crude enzyme broth and its applicability for the removal of bisphenol A

Abstract

Bisphenol A is an endocrine interfering compound, produced and used on a large scale worldwide. Chemical and biologic methods can be used to remove it from the environment. Biological methods are considered less costly, safer and, according to green chemistry defi nitions, an environmentally correct method. Considering the use of a crude enzyme broth, without any downstream process, the costs could be mostly reduced. Thus, the removal of bisphenol A by Pleurotus sajor-caju crude enzyme broth was investigated. Initially, the agro-industrial wastes were characterized and, the composition of the culture medium and the bioreactor culture conditions were defi ned.The enzyme produced in the highest concentration was characterized and the crude broth used in the bisphenol A removal assays. The OXI45 culture medium presented the highest laccase activity (1,850.7 U L -1 , 350 rpm). Greater laccase stability was observed at 20 -40 o C and pHs 5 -7. Vanillin and ferulic acid (considered mediator compounds) were identifi ed in the crude broth, probably helping on the obtention of the high value of removal effectiveness (0.052 mg U -1 h -1 ). The results indicate the potential use of the Pleurotus sajor-caju crude enzyme broth to obtain an enzymatic formulation for application in the environmental area.

Proteome Analysis and In Vitro Antiviral, Anticancer and Antioxidant Capacities of the Aqueous Extracts of Lentinula edodes and Pleurotus ostreatus Edible Mushrooms

“…In comparison to other edible mushrooms, oyster and shiitake mushrooms have a brief growing period and can be harvested throughout the year [ 14 ]. As a result of their ease of cultivation and testing, high nutritional values and promising medicinal benefits, these mushrooms have tremendous potential in the food and pharmaceutical industries [ 4 , 15 ]. Indeed, higher Basidiomycetes can be promising multifunctional food sources.…”