Antineoplastic potential and chemical evaluation of essential oils from leaves and flowers of Tagetes ostenii Hicken

Abstract

Breast and cervical cancer represent a major problem of women's global public health. Here, we investigated the chemical composition of essential oils from leaves and fl owers of T. ostenii and the antineoplastic potential in a cervical cancer and breast cancer cell line, SiHa and MCF-7; and non-tumoral cells, HaCat. The chemical analysis revealed a predominance of oxygenated monoterpenes in both essential oils. The IC50 after 24 h of treatment was 72 ng/mL for EO 1 and 83 ng/mL for EO 2 in SiHa cells. For MCF-7 the IC50 was 174.3 ng/mL for EO 1. For HaCat cells it was 54.45 ng/mL for EO 1 and 20.83 ng/mL for EO 2. A synergistic effect with both essential oils and cisplatin was also verifi ed. SiHa cells had their wound healing capacity reduced after 48 h of treatment with EO 2 and both essential oils were able to signifi cantly inhibit the adhesion process and clonogenic ability after 24 h of treatment. Our results suggest a long-lasting inhibitory activity in SiHa cells because of the low recovery capacity of cells after treatment withdrawal. Flow cytometer with annexin V/propidium iodide demonstrated a majority cell death through late apoptosis after 24, 48 and 72 hours of treatment.

Screening of Cu4O3 NPs efficacy and its anticancer potential against cervical cancer

“…58 Restricting cell adhesion and migration is a potential therapeutic approach to prevent tumors and metastases. 59,60 In this study, Cu 4 O 3 NPs showed a dosedependent delay in the complete closure of the scratched area, leading to decreased motility and migration of cancer cells (Figure 12). These findings indicate a potential role for Cu 4 O 3 NPs in cellular extracellular matrix connection disruption and tumor adhesion process blockage.…”

“…Cancer cells cannot grow in the absence of attachment because cell adhesion is a vital stage in various biological processes such as development, tissue structure maintenance, angiogenesis, and tumor propagation 58 . Restricting cell adhesion and migration is a potential therapeutic approach to prevent tumors and metastases 59,60 . In this study, Cu 4 O 3 NPs showed a dose‐dependent delay in the complete closure of the scratched area, leading to decreased motility and migration of cancer cells (Figure 12).…”

Antineoplastic Activity of Water-Soluble Form of Novel Kinase Inhibitor 1-(4-Chlorobenzyl)-3-chloro-4-(3-trifluoromethylphenylamino)-1H-pyrrole-2,5-dione immobilized on Polymeric Poly(PEGMA-co-DMM) Carrier

“…The clonogenic assay was conducted using the modified protocol described previously [39]. The HCT116 cells (750 cells/1 mL) were seeded in a 12-well plate.…”

“…The selectivity of action of chemopreventive agents is an important parameter of the pharmaceuticals that identifies their clinical potential. The higher selectivity index (SI) of compound indicates greater anticancer specificity and efficiency in cancer chemotherapy, as well as higher safety (smaller toxic effect towards normal cells) [39,47]. The compounds displaying the SI > 3.0 are considered to be highly selective [48].…”

“…The colony-forming capacity of cancer cells is an important parameter used for estimation of the anticancer activity of studied agents. Its measurement permits defining the fraction of tumor cells that retains a capacity to produce colonies that is an important phenotypic characteristics of tumor cells [39,49]. MI-1 compound in 0.1 µg/mL dose decreased (by 31.5%) the number of colonies formed by the carcinoma cells of HCT116 line from 162 (control) to 111, in 0.25 µg/mL dose-to 72 (by 55.6%), in 0.5 µg/mL dose-to 1 (by 99.4%), and in 1 µg/mL dose, there were no colonies of tumor cells detected (Figure 5).…”