Identification of caffeic acid and rutin by UHPLC MS/MS and antioxidant activity of Commelina erecta Lineu. in cell culture

Abstract

The Commelina erecta L. (C. erecta) also known as erva-de-santa-luzia is reported by local population to have medical properties against some pathological conditions. In this study, two extracts of C. erecta leaves (aqueous and ethanolic) were phytochemically analysed and evaluated for their in-vitro antioxidant activities by DPPH, TBARS, NO assays and cell viability assays. The ultra-high performance liquid chromatography followed by tandem mass spectrometry analysis showed the presence of rutin and caffeic acid in aqueous and ethanolic extract. The total polyphenols in aqueous and ethanolic extracts found were 142.7 ± 3.0 and 123.1 ± 5.8 μg/mL of GAE, respectively. The ethanolic extract (5 mg/mL) inhibits TBARS by 33.8%, and the aqueous extract (5 mg/mL) exhibited scavenger property against nitric oxide derivatives to an extent of 77.8%. In cell culture, both extracts improved cell survivability under H 2 O 2 induced oxidative stress. Thus, C. erecta extract is a good candidate to become a phytotherapic medicine.

Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil

“…However, in most of the analyzed samples, only traces of these compounds were found, being possible to quantify them only in the hydroethanolic extract obtained from the stem at the flowering stage. A fourth compound, namely rutin, has also been identified in previous studies of C. erecta leaves [25]. In the works of both Bezerra et al [26] and Otsuka et al [25], these compounds were only identified with no quantification being performed; thus, no comparison of values can be carried out.…”

“…A fourth compound, namely rutin, has also been identified in previous studies of C. erecta leaves [25]. In the works of both Bezerra et al [26] and Otsuka et al [25], these compounds were only identified with no quantification being performed; thus, no comparison of values can be carried out. Among the identified phenolic compounds (Table 3), it is noteworthy that several apigenin derivatives are reported for the first time in this species.…”

“…Recently, Otsuka et al [25] reported the presence of rutin and caffeic acid in the ethanolic and aqueous extracts of C. erecta leaves, and Bezerra et al [26] identified the presence of luteolin, isoquercitrin, quercitrin, shikimic acid, saccharinic acid lactone and three sterols in the ethyl acetate and ethanolic extracts of the stems. Moreover, the antioxidant activity [25,26] and antimicrobial activity by the agar-diffusion method [27] were evaluated for this species.…”

“…Recently, Otsuka et al [25] reported the presence of rutin and caffeic acid in the ethanolic and aqueous extracts of C. erecta leaves, and Bezerra et al [26] identified the presence of luteolin, isoquercitrin, quercitrin, shikimic acid, saccharinic acid lactone and three sterols in the ethyl acetate and ethanolic extracts of the stems. Moreover, the antioxidant activity [25,26] and antimicrobial activity by the agar-diffusion method [27] were evaluated for this species. Although being used by different communities in South America, the utilization of this species is based only on empirical knowledge because there is a scarcity of data on its nutritional and chemical composition as well as on its bioactive properties.…”

The Temporal and Spatial Variation of Arthropod Associations Inhabiting Non-Crop Vegetation in a Sisal Crop, Agave sisalana in the Caatinga Biome

“…showed molluscicidal activity against the snail Biomphalaria glabrata (Say), the intermediated host of Schistosoma mansoni Sambon (Diplostomida: Schistosomatidae) [27]. Moreover, several of the identified plants, such as C. erecta, Lourteigia ballotaefolia (Kunth) R.M.King and H.Rob., A. absinthium, Centratherum punctatun Cass., Sida spp., and Solanum lycocarpum A.St.-Hil., contain compounds with medical properties or have been traditionally used as medicinal plants [28][29][30][31][32][33][34].…”