In vitro anthelmintic activity of Psidium guajava hydroalcoholic extract against gastro-intestinal sheep nematodes

Abstract

Tanniferous plants have been used for ruminants verminosis control and represent a possibility to minimize the pharmacological resistance against conventional antiparasitics. This study aimed to evaluate the antihelminthic activity of the hydroalcoholic extract of stem bark of guava tree (PgHA). It was performed the hatchability and larval migration inhibition assays to evaluate PgHA at the following concentrations 0.62, 1.25, 2.5 and 5.0 mg mL-1 and the control treatments. The total polyphenol, fl avonoid and tannin contents were determined by phytochemical analysis, high performance liquid chromatography coupled to mass spectrometry. The antioxidant activity was evaluated by 1,1-diphenyl-2-picrylhydrazyl, ferric reducing antioxidant power and thiobarbituric acid reactive substances tests. It was also determinated total protein, intracellular H 2 O 2 and antioxidant activity of enzimes: glutathione S-transferase and superoxide dismutase. PgHA was able to inhibit both hatchability and larval migration, but only hatchability inhibition presented dose-dependent pattern. The antioxidant activity was demonstrated by linear regression with IC 50 corresponding to 534.02 μg mL-1. The antiparasitic mechanism occurred through pro-oxidative activity by the increase of total proteins, intracellular H 2 O 2 and the lipid peroxidation products, as well as the increase of the enzymes above related. Thus, the PgHA showed antiparasitic activity in vitro.

Pharmacognostical, biochemical activities and zootechnical applications of Psidium guajava (Myrtaceae), plant with high medicinal value in tropical and subtropical parts of the World: A review

“…Very few authors have studied the anthelmintic activity of Psidium guajava [43][44][45][46][47] . The common methods used for in vitro evaluation of anthelmintic activity of plant extracts are: egg hatch test, adult worm mobility test, and larval migration inhibition test.…”

“…The common methods used for in vitro evaluation of anthelmintic activity of plant extracts are: egg hatch test, adult worm mobility test, and larval migration inhibition test. The helminthes used are Haemonchus contortus nematode of small ruminants [44,47] and Phaeritima posthuma study model of human helminthes [43] . According to the results of [47] , hydroalcoholic extracts of Psdium guajava stem barks act much more on the eggs by preventing their hatching.…”

“…The helminthes used are Haemonchus contortus nematode of small ruminants [44,47] and Phaeritima posthuma study model of human helminthes [43] . According to the results of [47] , hydroalcoholic extracts of Psdium guajava stem barks act much more on the eggs by preventing their hatching. This has the advantage of suspending the life cycle of the parasite and limiting reinfestations and contamination of the pasture.…”