Somatic embryogenesis from leaf tissues of macaw palm [Acrocomia aculeata (Jacq.) Lodd. ex Mart.]

Meira, Filipe Sathler; Luis, Zanderluce Gomes; Cardoso, InaÊ MariÊ A S; Scherwinski‐Pereira, Jonny Everson

doi:10.1590/0001-3765202020180709

Cited by 11 publications

(3 citation statements)

References 46 publications

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“…Triclopyr, a common herbicide, is quickly translocated in the plant, mainly through the symplastic route, which allows the selective absorption of solutes. It then accumulates in meristematic tissues (Cessna et al ., 2002), which are poorly differentiated and/or undifferentiated, and contain intensely dividing cells with large nuclei and dense cytoplasm (Gomes et al ., 2017; Meira et al ., 2020), as noted here in cells treated with triclopyr (100 µM).…”

Section: Discussionmentioning

confidence: 99%

Optimization of somatic embryogenesis inEuterpe edulisMartius using auxin analogs and atomic force microscopy

Mello

Taliuli

Silva

et al. 2023

Preprint

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Euterpe edulisMartius is an endangered species of the Atlantic Forest, whose fruits have high antioxidant potential, and propagated exclusively by seeds. The present study assessed the ability of different auxin inducers and picloram analogs to trigger somatic embryogenesis inE. edulis. Immature seeds were harvested, and their zygotic embryos were excised and grown in MS culture medium supplemented with 2,4-D dichlorophenoxyacetic acid (2,4-D) or picloram at 150, 300, 450, 600 µM. The activity of picloram analogs triclopyr and clopyralid was evaluated in semisolid MS medium. At maturation and germination, picloram-derived calli and somatic embryos isolated from triclopyr-grown cultures were first transferred to pre-maturation medium and, after 30 days, to basal MS or MS medium supplemented with either 5 µM abscisic acid or 0.53 µM 1-naphthaleneacetic acid plus 12.3 µM 2-isopentenyladenine. Finally, somatic embryos with root protrusions were transferred to MS medium devoid of sucrose for 30 days and then acclimatized ex vitro. Scanning, transmission, and atomic force microscopy revealed that picloram was superior to 2,4-D but less effective than triclopyr (100 µM) in inducing embryogenesis. Maturation and germination of somatic embryos in E. edulis can be maximized by 5 µM abscisic acid, and selecting calli via atomic force microscopy.HighlightThis work opens novel roads for embryogenic induction, using a new and more efficient inducer than the usual ones, and an innovative evaluation technique based on AFM.

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Section: Discussionmentioning

confidence: 99%

Optimization of somatic embryogenesis inEuterpe edulisMartius using auxin analogs and atomic force microscopy

Mello

Taliuli

Silva

et al. 2023

Preprint

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“…Setelah 8 minggu dikulturkan, bagian eksplan yang mengalami pelukaan dan menebal mulai membentuk kalus kompak berwarna hijau dan kuning (Gambar 2a c). Pada tanaman Acrocomia aculeata kalus berkembang lebih lama lagi , setelah 4 -6 bulan pada media induksi yang mengandung picloram (Meira et al, 2020) Dari penelitian ini tidak semua eksplan membentuk kalus tetapi kalus hanya diperoleh dari bagian eksplan yang mengalami pelukaan (Gambar 1 b) yang diawali dengan penebalan. Hapsoro et al, (2020) juga melaporkan bahwa penebalan dan induksi kalus pada kopi robusta terjadi akibat pelukaan pada bagian tanaman.…”

Section: A Pengaruh Jenis Auksinunclassified

“…Pada tanaman monokotil macam palm (Acrocomia aculeata (Jack.) penggunaan picloram dengan konsentrasi 450 µM (10,8 mg/L) dihasilkan kalus embriogenik yang berpotensi membentuk embrio somatik (Meira et al, 2020). Rata-rata embrio somatik globular yang dihasilkan dari perlakuan ini adalah sebanyak 10.4 embrio.…”

Section: A Pengaruh Jenis Auksinunclassified

Embentukan Embrio Somatik Dari Eksplan Daun Ramin, Spesies Tanaman Langka

Yelnititis

2021

JPTH

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Ramin (Gonystylus bancanus (Miq.) Kurz) is one of the most valuable timbers in Indonesia and the most over-exploited woody species, on the other hand,the success in propagation techniques are still limited. This species has been listed in Appendix II of CITES since 2004as the number of trees and populations continuouslydecline. Tissue culture has been explored for mass propagation, however, this technique still facesthechallenge, mainly in shoot elongation regenerated from single node explants. The purpose of these experiments is to selectthe best auxin (2,4-D, picloram,dan dicamba) and the best concentration of Benzyl Adenine(BA) treatment on somaticembryo formation. A series of experiments of somatic embryogenesis from leaf explant for ramin were conducted at Tissue Culture Laboratory, Center for Forest Biotechnology and Tree Improvement Yogyakarta. Modified Murashige and Skoog (MS) media supplemented with 0,1 mg/Lthiamine; 0,5 mg/l nicotinic acid; 0,5 mg/Lpyridoxine; 2.0 mg/Lglycineand 100 mg/Lmyo-inositolwere used as growth medium. In thisstudy, three different auxins were used as treatments: 2,4-D(2,4 dichlorophenoxy acetic acid), picloram,and dicamba appliedat6.0 mg/L. The observation was made on the texture of callus formed and the performance of the somatic embryos obtained. The results showed thatthe texture of callus obtained is compact formed callus and green in color. The best treatment to induce globular somatic embryos is using 6.0 mg/Lpicloram within eight months. Thebest treatment to induce globular and torpedo somatic embryos is BA 3.0 mg/L treatment

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Cellular responses of oil palm genotypes during somatic embryogenesis involve participation of procambial cells, DNA demethylation, and auxin accumulation

2022

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Somatic embryogenesis from leaf tissues of macaw palm [Acrocomia aculeata (Jacq.) Lodd. ex Mart.]

Cited by 11 publications

References 46 publications

Optimization of somatic embryogenesis inEuterpe edulisMartius using auxin analogs and atomic force microscopy

Optimization of somatic embryogenesis inEuterpe edulisMartius using auxin analogs and atomic force microscopy

Embentukan Embrio Somatik Dari Eksplan Daun Ramin, Spesies Tanaman Langka

Cellular responses of oil palm genotypes during somatic embryogenesis involve participation of procambial cells, DNA demethylation, and auxin accumulation

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