Identification of Listeria monocytogenes in cattle meat using biochemical methods and amplification of the hemolysin gene

Silva, Alessandra S; Duarte, Elizabeth Amélia Alves; Oliveira, Thiago Alves Santos de; Evangelista-Barreto, Norma Suely

doi:10.1590/0001-3765202020180557

Cited by 4 publications

(1 citation statement)

References 33 publications

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“…Municipal fairs are places with specific characteristics that have favorable conditions for the growth and proliferation of microorganisms in their environment (Silva et al, 2020). It is known that the main sources of contamination of food are: raw material (including water), environment (air, equipment, packaging and miscellaneous materials), and personnel (food handling) (Clark;Crandall;Reynolds, 2019).…”

Section: Resultsmentioning

confidence: 99%

Impact of different marketing conditions on the bacteriological quality of meat products

Silva

Cunha

Silva³

et al. 2022

RSD

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Animal foods, such as meat and meat products, are among the main sources of infection and spread of Foodborne Diseases, where marketing conditions are an important factor to be evaluated. Forty-eight samples of meat products sold under outdoor fairs, butcher shops and supermarkets were collected. The presence of microorganisms indicating hygienic and sanitary quality (fecal coliforms and Clostridium perfringens) was investigated, as well as the species Pseudomonas aeruginosa. The results showed a high presence of C. perfringens in 83.33% of the samples, fecal coliforms in 73% and P. aeruginosa in 100% of meat products, in all commercial points. As for marketing conditions, the difference in the food contamination index was significant (p ≤ 0.05) only for C. perfringens (X2 (2) = 8.5; p= 0.0143), where meat sold in fairs were more susceptible to contamination by C. perfringens than meats sold in supermarkets. The meat products analyzed did not show quality from a bacteriological point of view, given the high presence of fecal coliforms, P. aeruginosa and C. perfringens, leading to question mainly the form of trade that can generate unsafe products for consumption.

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Section: Resultsmentioning

confidence: 99%

Impact of different marketing conditions on the bacteriological quality of meat products

Silva

Cunha

Silva³

et al. 2022

RSD

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Listeria monocytogenes an Emerging Pathogen: a Comprehensive Overview on Listeriosis, Virulence Determinants, Detection, and Anti-Listerial Interventions

et al. 2023

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Nonhemolytic Listeria monocytogenes—Prevalence Rate, Reasons Underlying Atypical Phenotype, and Methods for Accurate Hemolysis Assessment

2022

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Listeria monocytogenes is a foodborne pathogen that typically presents β-hemolytic activity. However, there are literature reports indicating that L. monocytogenes strains are sometimes nonhemolytic or their zones of hemolysis are perceivable only after removal of the colonies from the agar plate. Nonhemolytic L. monocytogenes are most commonly encountered in food products, but some have also been detected in clinical samples. Usually, atypical bacteria of this species belong to serotype 1/2a. Mutations of the prfA gene sequence are the most common reason for changed phenotype, and mutations of the hly gene are the second most common cause. There are also reports that the methodology used for detecting hemolysis may influence the results. Sheep or horse blood, although most commonly used in modern studies, may not allow for the production of clear hemolytic zones on blood agar, whereas other types of blood (guinea pig, rabbit, piglet, and human) are more suitable according to some studies. Furthermore, the standard blood agar plate technique is less sensitive than its modifications such as bilayer or top-layer (overlay) techniques. The microplate technique (employing erythrocyte suspensions) is probably the most informative when assessing listerial hemolysis and is the least susceptible to subjective interpretation.

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Identification of Listeria monocytogenes in cattle meat using biochemical methods and amplification of the hemolysin gene

Cited by 4 publications

References 33 publications

Impact of different marketing conditions on the bacteriological quality of meat products

Impact of different marketing conditions on the bacteriological quality of meat products

Listeria monocytogenes an Emerging Pathogen: a Comprehensive Overview on Listeriosis, Virulence Determinants, Detection, and Anti-Listerial Interventions

Nonhemolytic Listeria monocytogenes—Prevalence Rate, Reasons Underlying Atypical Phenotype, and Methods for Accurate Hemolysis Assessment

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