Inhibitory effect of Lonchocarpus araripensis lectin in rat acute models of inflammation

Abstract

Dalbergieae tribe lectins, possessing binding affinity for galactose and mannose, present inflammatory and nociceptive effects, while those for N-acetylglucosamine are anti-inflammatory. Since the anti-inflammatory effect of the seed lectin of L. araripensis (LAL) had been already demonstrated in mice, this effect was presently evaluated in rat models of acute inflammation. LAL (0.01-1 mg/kg) was administered by intravenous (i.v.) route in male Wistar rats 30 min before paw edema induction by dextran or carrageenan, and peritonitis by carrageenan. LAL (1 mg/kg) was incubated with N-acetylglucosamine for allowing lectin-sugar interactions before injection into animals. LAL toxicity was evaluated by the parameters: body mass, organs weight, stomach macroscopy, hematological and biochemical dosage. Statistical analysis was performed by ANOVA and Bonferroni's test (p<0.05). The paw edema induced by carrageenan (AUC: 0.96 ± 0.09) was inhibited by LAL about 39% (0-2 h) at all doses, and about 72% (3-5 h) at 0.1 and 1 mg/kg. The increase in the neutrophil migration stimulated by carrageenan was also inhibited by LAL (83%). In both models, LAL inhibitory effect was prevented by GlcNAc. The subchronic treatment with LAL was well tolerated by animals. LAL possesses anti-inflammatory effect via lectin domain, indicating potential modulator role in cellular inflammatory events.

The anti-inflamatory effect of Andira anthelmia lectin in rats involves inhibition of the prostanoid pathway, TNF-α and lectin domain

“…The differential leukocyte count showed that neutrophils are the principal target cells involved in the anti-in ammatory effect of AAL. Similar results have been observed in rats for the lectins isolated (Dalbergieae tribe) from Lonchocarpus sericeus [17,18] and L. araripensis [19], presenting binding a nity for N-acetylglucosamine. In addition, the interference of AAL in neutrophil migration was also con rmed by the reduced MPO activity in the paw, a heme-containing enzyme that is abundantly expressed in neutrophils.…”

“…Accordingly, the antiin ammatory effect of leguminous lectins is characterized by the preferential inhibition on cellular events of acute in ammation [21,29,30]. Other isolated lectins belonging to the Dalbergieae tribe also showed inhibitory effect on carrageenan-induced paw edema such as that of L. sericeus [17][18], L. araripensis [30] and L. campestris [19].…”

Antinociceptive and Anti‐Inflammatory Effects of Saline Extract and Lectin‐Rich Fraction from Microgramma vacciniifolia Rhizome in Mice

“…These proteins can modulate several processes like infection, cell recognition, cell communication, and various intracellular responses [14,15] . Plant lectins have shown anti‐inflammatory [16,17] and antinociceptive [18,19] effects. For example, some studies have proposed that lectins can modulate inflammation and pain through interactions of their carbohydrate‐binding domains with glycosylated selectin‐binding sites [20] or δ‐ and κ‐receptors, [21] respectively.…”

The Antinociceptive Effect of Lonchocarpus Araripensis Lectin is Mediated by Endocannabinoid Receptors

“…The lectin isolated from Lonchocarpus araripensis (LAL), belonging to Dalbergieae tribe, recognizes and specifically binds to N-acetyl-D-glucosamin [2]. Several effects have already been evidenced for LAL, such as vasorelaxant [3], anti-inflammatory [2,4], and antinociceptive [5,6], also a protective effect on sepsis model [7]. In respect to the antinociceptive activity, LAL has a pleiotropic effect in several nociceptive pathways, being associated to the modulation of inflammatory and/or hypernociceptive mediators [5,6].…”