Somatic embryogenesis in the commercial papaya hybrid UENF/Caliman 01 relying on plantlet production from sexed adult hermaphrodite donor plants

Gouvea, Drielly S; Chagas, Kristhiano; Cipriano, Jamile L.D.; Lopes, José Carlos; Schmildt, Edílson Romais; Otoni, Wagner Campos; Schmildt, Omar; Araújo, Caroline Palacio de; Alexandre, Rodrigo Sobreira

doi:10.1590/0001-3765201920180504

Cited by 4 publications

(3 citation statements)

References 50 publications

(36 reference statements)

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“…Plant regeneration obtained from single flower and petal was 37.5 and 12.5 %, respectively (Table 2). GA3 has been reported to facilitate germination of somatic embryos in in vitro conditions of many plant species [31][32][33]. However, the response to its concentration was quite different depended on initial cultured explant and plant species as well.…”

Section: Effects Of Types Of Explants and Ga3 On Development Of Sementioning

confidence: 99%

Somatic Embryo (SE) Formation from Culturing Floral Explants of Rubber Tree (Hevea brasiliensis Muell. Arg.) and Assessment of Genetic Stability by RAPD and SSR Markers

Tongtape,

Te-chato,

Yenchon

2023

Trends Sci

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Rubber tree is economically important rubber producing plant of Thailand. At present, a rubber tree plantation is susceptible to white root disease. Therefore, the use of rootstock from early introduce clone that proved to be resistant to white root disease could help sustain growing of rubber tree. Thus, the objectives of this research were to study the effects of plant growth regulators and different explants on somatic embryo (SE) induction of this rubber clone and assessment genetic stability. The results revealed that mix flower explant cultured on MS medium supplemented with 2.0 mg/L 6-benzyladenine (BA) and 1.5 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D) provided callus subsequent somatic embryo (SE) formation at the highest frequency of 39.84 % and number of cotyledonary embryos (CEs) at 3.25 embryos /callus after 3 passages of subculture in the same culture medium (4 weeks/passage). SEs germinated into embryonic axis at 50 % and further development into shoot at 25 % after transfer to 0.25 mg/L GA3 containing MS medium with the best concentrations of BA and 2,4-D for 4 weeks. The assessment gene stability by RAPD and SSR markers showed no variation between mother plant and in vitro plantlets. In this work, a novel explant source - the floral section of the rubber tree - was used for the first time in order to design an effective technique for in vitro somatic embryogenesis. HIGHLIGHTS The first report on plantlets regeneration using floral explant of rubber tree This mother plant is proved to be resistance to Phytopthora leaf fall and white root diseases Assessment genetic stability of regenerated plantlets by SSR and RAPD markers GRAPHICAL ABSTRACT

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Section: Effects Of Types Of Explants and Ga3 On Development Of Sementioning

confidence: 99%

Somatic Embryo (SE) Formation from Culturing Floral Explants of Rubber Tree (Hevea brasiliensis Muell. Arg.) and Assessment of Genetic Stability by RAPD and SSR Markers

Tongtape,

Te-chato,

Yenchon

2023

Trends Sci

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“…Thus, somatic embryogenesis (SE) in papaya can be applied to produce clones on a large-scale, reducing production costs due to the sexual segregation presented by seedlings [2]. The SE in C. papaya already has a well-established and characterized protocol able to generate globular, heart, torpedo, and cotyledonary embryo stages, similar to those of zygotic embryogenesis [3]. Moreover, SE in papaya also serves as an efficient experimental model for genetic, physiological, and morphological studies of the formation and development of somatic embryos in plants [4,5].…”

Section: Introductionmentioning

confidence: 99%

Time-Dependent Proteomic Signatures Associated with Embryogenic Callus Induction in Carica papaya L.

Xavier,

Corrêa,

da Paschoa

et al. 2023

Plants

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Sex segregation increases the cost of Carica papaya production through seed-based propagation. Therefore, in vitro techniques are an attractive option for clonal propagation, especially of hermaphroditic plants. Here, we performed a temporal analysis of the proteome of C. papaya calli aiming to identify the key players involved in embryogenic callus formation. Mature zygotic embryos used as explants were treated with 20 μM 2,4-dichlorophenoxyacetic acid to induce embryogenic callus. Total proteins were extracted from explants at 0 (zygotic embryo) and after 7, 14, and 21 days of induction. A total of 1407 proteins were identified using a bottom-up proteomic approach. The clustering analysis revealed four distinct patterns of protein accumulation throughout callus induction. Proteins related to seed maturation and storage are abundant in the explant before induction, decreasing as callus formation progresses. Carbohydrate and amino acid metabolisms, aerobic respiration, and protein catabolic processes were enriched throughout days of callus induction. Protein kinases associated with auxin responses, such as SKP1-like proteins 1B, accumulated in response to callus induction. Additionally, regulatory proteins, including histone deacetylase (HD2C) and argonaute 1 (AGO1), were more abundant at 7 days, suggesting their role in the acquisition of embryogenic competence. Predicted protein–protein networks revealed the regulatory role of proteins 14-3-3 accumulated during callus induction and the association of proteins involved in oxidative phosphorylation and hormone response. Our findings emphasize the modulation of the proteome during embryogenic callus initiation and identify regulatory proteins that might be involved in the activation of this process.

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“…4-CPA is usually used to prevent falling flowers and fruits, promote early maturity, and improve the quality of plants. For these reasons, it is commonly used as a plant growth regulator for tomatoes, bean sprouts, and other vegetables. − Also, it serves as the pharmaceutical intermediate of meclofenoxate, which is used as a central nervous stimulant for consciousness disorder and traumatic coma . Also, 4-CPA has been proved to be used to prepare biofilms with strong ion-exchange capacity (IEC) and proton conductivity.…”

Section: Introductionmentioning

confidence: 99%

Solubility Measurement and Data Correlation of 4-Chlorophenoxyacetic Acid in 13 Monosolvents at Temperatures from 283.15 to 328.15 K

et al. 2021

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The solubility of 4-chlorophenoxyacetic acid (4-CPA) in 13 monosolvents including ethanol, 1-propanol, isopropanol, 1-butanol, 2-butanol, isobutanol, ethyl formate, methyl acetate, ethyl acetate, propyl acetate, butyl acetate, acetonitrile, and 1,4-dioxane was measured by a gravimetric method from 283.15 to 328.15 K at an atmospheric pressure of 0.1013 MPa. The solubility of 4-CPA increased with temperature and followed the order 1,4-dioxane > alcohols > esters > acetonitrile at all experimental temperatures. The solvent effects, especially solvent polarity, hydrogen bond donor propensity, and hydrogen bond acceptor propensity, were used to analyze the solubility of 4-CPA in different solvents. The solubility basically follows the rule "like dissolves like" and has a higher value in solvents with strong hydrogen bond donor propensity and hydrogen bond acceptor propensity. Besides, the modified Apelblat equation, λh equation, and van't Hoff equation were applied to correlate the measured solubility data. The average relative deviation (ARD) values showed that the correlation results of the three models agree well with the experimental data.

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Somatic embryogenesis in the commercial papaya hybrid UENF/Caliman 01 relying on plantlet production from sexed adult hermaphrodite donor plants

Abstract: Somatic embryogenesis in the commercial papaya hybrid UeNF/ Caliman 01 relying on plantlet production from sexed adult hermaphrodite donor plants. An Acad Bras Cienc 91: e20180504.

Cited by 4 publications

References 50 publications

Somatic Embryo (SE) Formation from Culturing Floral Explants of Rubber Tree (Hevea brasiliensis Muell. Arg.) and Assessment of Genetic Stability by RAPD and SSR Markers

Somatic Embryo (SE) Formation from Culturing Floral Explants of Rubber Tree (Hevea brasiliensis Muell. Arg.) and Assessment of Genetic Stability by RAPD and SSR Markers

Time-Dependent Proteomic Signatures Associated with Embryogenic Callus Induction in Carica papaya L.

Solubility Measurement and Data Correlation of 4-Chlorophenoxyacetic Acid in 13 Monosolvents at Temperatures from 283.15 to 328.15 K

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